Results 61 to 70 of about 6,321 (156)

Characterization of the Lipid Profile of Immature and In Vitro Matured Cat Oocytes

Molecular Reproduction and Development, Volume 92, Issue 8, August 2025.
ABSTRACT Forskolin (FSK), l‐carnitine (LC), and Conjugated Linoleic Acid (CLA) are lipid modulators that reduce cellular lipid content. This study characterizes the lipid profile and mRNA content of immature and in vitro matured cat oocytes with or without a MIX of these modulators. Ovaries were collected, COC retrieved, and allocated into three groups:
Erlandia M. Vasconcelos, Thais G. de Oliveira, Ribrio I. T. P. Batista, Paulo S. C. Rangel, Georgia C. Atella, Gabriela R. Leal, Joanna M. G. Souza‐Fabjan   +6 more
wiley   +1 more source

Gene Expression Studies in Formalin-Fixed Paraffin-Embedded Samples of Cutaneous Cancer: The Need for Reference Genes

Current Issues in Molecular Biology, 2021
Formalin-fixed paraffin-embedded (FFPE) tumour samples may provide crucial data regarding biomarkers for neoplasm progression. Analysis of gene expression is frequently used for this purpose.
Omar García-Pérez, Leticia Melgar-Vilaplana, Elizabeth Córdoba-Lanús, Ricardo Fernández-de-Misa   +3 more
doaj   +1 more source

Differences in the suitability of published honey bee (Apis mellifera) reference genes between the African subspecies Apis mellifera scutellata and European derived Apis mellifera

Molecular Ecology, Volume 34, Issue 15, August 2025.
Abstract Quantitative real‐time polymerase chain reaction (qPCR) is a method widely used to determine changes and differences in gene expression. As target gene expression is most often quantified relative to the expression of reference genes, the validation of suitable reference genes is of critical importance.
Anja Buttstedt, Christian W. W. Pirk, Abdullahi A. Yusuf   +2 more
wiley   +1 more source

Study of Some Reference Genes Expression Stability in Rice Using Real-Time PCR Method Under Biotic Stress [PDF]

مجله بیوتکنولوژی کشاورزی, 2019
Objective Gene expression studies by Real-Time PCR constitute a powerful tool to analyze the mechanisms underlying plant biotic-stress tolerance. One of the crucial steps of this technique is the selection and validation of reference genes to normalize ...
Hamidreza Ghorbani, Seyyed Hamidreza Hashemi-petroudi, Mehdi Rostami Rostami   +2 more
doaj   +1 more source

Housekeeping gene expression variability in differentiating and non-differentiating 3T3-L1 cells

Adipocyte, 2023
Normalization is a crucial step in gene expression analysis to avoid misinterpretation. Reverse transcription-quantitative polymerase chain reaction was used to measure the expression of 10 candidate housekeeping genes in non-differentiated (ND) and ...
Danang Dwi Cahyadi, Tomoko Warita, Nanami Irie, Kana Mizoguchi, Jiro Tashiro, Yoshinao Z. Hosaka, Katsuhiko Warita   +6 more
doaj   +1 more source

Identification of stable reference genes for quantitative PCR in koalas [PDF]

, 2018
To better understand host and immune response to diseases, gene expression studies require identification of reference genes with stable expression for accurate normalisation.
A Kappel, A Radonic, B Artegiani, CA Waugh, CL Andersen, D Kim, DG Ginzinger, F Almeida-Oliveira, F Bartz, G Bamias, GS Meers, GS Simmons, IE Maher, IE Maher, J Vandesompele, J Woinarski, J Yperman, JJ Hanger, K Ahn, KC Thomas, KM Morris, M Kubista, M Pertea, MA Valasek, MW Pfaffl, N Silver, OT Ong, P Brym, PD Lee, PJ Canfield, QL Zhang, R Tarlinton, R Tarlinton, RK Das, S Bages, SA Bustin, SA Bustin, T Burmeister, T Nolan, V Gonzalez-Astudillo, Y Zhang   +40 more
core   +4 more sources

Highly Targeted Metabolomics Coupled With Gene Expression Analysis by RT–qPCR Improves Beef Separation Based on Grass, Grain, or Grape Supplemented Diet

Food Frontiers, Volume 6, Issue 3, Page 1483-1497, May 2025.
ABSTRACT The objective of this study was to use a multi‐omics (i.e., gene expression quantification, metabolomics, and fatty acid [FA] profiling) approach to separate and authenticate beef from three different dietary groups. In this 2‐year study, Red Angus steers (n = 54) were randomly allocated to one of three treatments: (1) complex biodiverse ...
Lucas Krusinski, Chloe Castanon, Rosalee S. Hellberg, Isabella C. F. Maciel, Muhammad Ahsin, Stephan van Vliet, Jason E. Rowntree, Jenifer I. Fenton   +7 more
wiley   +1 more source

Stable reference genes for the measurement of transcript abundance during larval caste development in the honeybee [PDF]

, 2013
Many genes are differentially regulated by caste development in the honeybee. Identifying and understanding these differences is key to discovering the mechanisms underlying this process.
A Lourenco, A Patel, AR Barchuk, B Scharlaken, CL Andersen, DE Wheeler, DW Severson, Elizabeth J. Duncan, F Wolschin, FC Humann, G-H Wang, GV Glass, H Holzl, H Stabe, J Hellemans, J Vandesompele, JD Evans, JD Evans, LM Lix, M Boer de, M Corona, M Okoniewski, M Sardiello, MA Valasek, MW Pfaffl, NS Mutti, Peter K. Dearden, R Kucharski, Rosannah C. Cameron, S Rozen, SA Bustin, SV Azevedo, SV Azevedo de, X Chen   +33 more
core   +3 more sources

Pathogen and Gene Expression Profiles of Atlantic Salmon From an Endangered Population

Aquatic Conservation: Marine and Freshwater Ecosystems, Volume 35, Issue 4, April 2025.
ABSTRACT The role of pathogens in impacting the behaviour and fate of salmonids has been studied extensively for some selected pathogens such as sea lice. However, the whole pathobiome of the fish are seldom considered and may confound and influence the study species in situ.
Robert J. Lennox, Angela D. Schulze, Kristi M. Miller, Trond Einar Isaksen, Eirik Straume Normann, Cecilie Iden Nilsen, Lotte S. Dahlmo, Bjørn T. Barlaup, Elisabeth Stöger, Sindre H. Eldøy, Knut Wiik Vollset   +10 more
wiley   +1 more source

Validating reference genes using minimally transformed qpcr data: findings in human cortex and outcomes in schizophrenia [PDF]

, 2016
BACKGROUND: It is common practice, when using quantitative real time polymerase chain reaction (qPCR), to normalise levels of mRNA to reference gene mRNA which, by definition, should not vary between tissue, with any disease aetiology or after drug ...
Brian Dean, Elizabeth Scarr, Madhara Udawela   +2 more
core   +4 more sources