Results 61 to 70 of about 7,909 (199)

A transcriptomic atlas facilitating systems biology approaches in pea

Crop Science, Volume 65, Issue 6, November/December 2025.
Abstract Pea is a model organism in biology and an agronomic crop, entering the genomics era. Although RNA‐sequencing (RNA‐seq) has become the main transcriptomic approach in biology, there is currently no genome‐wide expression atlas covering a wide range of biological conditions for this species.
Gatepe Cedoine Kodjovi, Mathias Coulon, Lisa Couturier, Amélie Morin, Ingrid Goma‐Louamba, Caroline Artault, Guillaume Tcherkez, Cécile Vriet, Sylvain La Camera, Nathalie Pourtau, Bouziane Moumen, Joan Doidy   +11 more
wiley   +1 more source

Identification of stable reference genes for quantitative PCR in koalas [PDF]

, 2018
To better understand host and immune response to diseases, gene expression studies require identification of reference genes with stable expression for accurate normalisation.
A Kappel, A Radonic, B Artegiani, CA Waugh, CL Andersen, D Kim, DG Ginzinger, F Almeida-Oliveira, F Bartz, G Bamias, GS Meers, GS Simmons, IE Maher, IE Maher, J Vandesompele, J Woinarski, J Yperman, JJ Hanger, K Ahn, KC Thomas, KM Morris, M Kubista, M Pertea, MA Valasek, MW Pfaffl, N Silver, OT Ong, P Brym, PD Lee, PJ Canfield, QL Zhang, R Tarlinton, R Tarlinton, RK Das, S Bages, SA Bustin, SA Bustin, T Burmeister, T Nolan, V Gonzalez-Astudillo, Y Zhang   +40 more
core   +4 more sources

Evaluation of reference genes for quantitative expression analysis in Mylabris sibirica (Coleoptera, Meloidae)

Frontiers in Physiology
Mylabris sibirica is a hypermetamorphic insect whose adults feed on oilseed rape. However, due to a shortage of effective and appropriate endogenous references, studies on molecular functional genes in Mylabris sibirica, have been tremendously limited ...
Chenhui Shen, Min Tang, Xiaofei Li, Li Zhu, Wei Li, Pan Deng, Qing Zhai, Gang Wu, Xiaohong Yan   +8 more
semanticscholar   +1 more source

Fibre‐Enriched High‐Carbohydrate (FEHC) Diet Modulates Inflammation Without Affecting Bone Health in Older Women With Obesity: A Randomised Clinical Trial

Diabetes/Metabolism Research and Reviews, Volume 41, Issue 7, October 2025.
ABSTRACT Background In older adults, obesity is associated with frailty, conditions worsened by age related decline in bone mineral density (BMD) and muscle mass. Objective To evaluate whether a 3‐month Fibre‐Enriched High Carbohydrate (FEHC) diet preserves bone health, reduces inflammation and modulates Wnt signalling in older adults with obesity ...
Francesca Cannata, Viola Viola, Giulia Leanza, Alice Laudisio, Malak Faraj, Flavia Tramontana, Alessandra Piccoli, Rocky Strollo, Mauro Maccarrone, Fabrizio Russo, Gianluca Vadalà, Veronica Sansoni, Giovanni Lombardi, Giuseppe Banfi, Chiara Verdelli, Rocco Papalia, Nicola Napoli   +16 more
wiley   +1 more source

Influence of heat stress, sex and genetic groups on reference genes stability in muscle tissue of chicken. [PDF]

PLoS ONE, 2017
Quantitative RT-PCR is an important technique for assessing gene expression. However, a proper normalization of reference genes prior to expression analyses of target genes is necessary.
Haniel Cedraz de Oliveira, Antonio Amandio Pinto Garcia, Juliana Gracielle Gonzaga Gromboni, Ronaldo Vasconcelos Farias Filho, Carlos Souza do Nascimento, Amauri Arias Wenceslau   +5 more
doaj   +1 more source

Stable reference genes for the measurement of transcript abundance during larval caste development in the honeybee [PDF]

, 2013
Many genes are differentially regulated by caste development in the honeybee. Identifying and understanding these differences is key to discovering the mechanisms underlying this process.
A Lourenco, A Patel, AR Barchuk, B Scharlaken, CL Andersen, DE Wheeler, DW Severson, Elizabeth J. Duncan, F Wolschin, FC Humann, G-H Wang, GV Glass, H Holzl, H Stabe, J Hellemans, J Vandesompele, JD Evans, JD Evans, LM Lix, M Boer de, M Corona, M Okoniewski, M Sardiello, MA Valasek, MW Pfaffl, NS Mutti, Peter K. Dearden, R Kucharski, Rosannah C. Cameron, S Rozen, SA Bustin, SV Azevedo, SV Azevedo de, X Chen   +33 more
core   +3 more sources

Validation of reference gene stability for normalization of RT-qPCR in Phytophthora capsici Leonian during its interaction with Piper nigrum L.

Scientific Reports
The selection of stable reference genes for the normalization of reverse transcription quantitative real-time PCR (RT-qPCR) is generally overlooked despite being the crucial element in determining the accuracy of the relative expression of genes.
Saranya Vijayakumar, Manjula Sakuntala
semanticscholar   +1 more source

Beet Chlorosis Virus Infection Mitigates Aphid‐Induced Plant Defences and Improves Plant Acceptability to Aphid Vectors

Molecular Ecology, Volume 34, Issue 20, October 2025.
ABSTRACT Plant viruses often alter host traits in ways that affect interactions with herbivores, potentially facilitating their own acquisition and transmission by insect vectors. However, little is known about the molecular mechanisms underlying this phenomenon.
Thomas Armand, Sylvaine Boissinot, Alessandra Maia‐Grondard, Philippe Hugueney, Véronique Brault, Quentin Chesnais   +5 more
wiley   +1 more source

Validating reference genes using minimally transformed qpcr data: findings in human cortex and outcomes in schizophrenia [PDF]

, 2016
BACKGROUND: It is common practice, when using quantitative real time polymerase chain reaction (qPCR), to normalise levels of mRNA to reference gene mRNA which, by definition, should not vary between tissue, with any disease aetiology or after drug ...
Brian Dean, Elizabeth Scarr, Madhara Udawela   +2 more
core   +4 more sources

Selection and Validation of qRT-PCR Internal Reference Genes to Study Flower Color Formation in Camellia impressinervis

International Journal of Molecular Sciences
Real-time quantitative PCR (qRT-PCR) is a pivotal technique for gene expression analysis. To ensure reliable and accurate results, the internal reference genes must exhibit stable expression across varied experimental conditions.
Peilan Zhang, Shuying Chen, Siyu Chen, Yuanming Zhu, Yuqing Lin, Xinyu Xu, Zhong-jian Liu, Shuangquan Zou   +7 more
semanticscholar   +1 more source