Results 71 to 80 of about 7,909 (199)

Selection of suitable reference genes for quantitative real-time polymerase chain reaction in human meningiomas and arachnoidea

BMC Research Notes, 2011
Findings At first 32 housekeeping genes were analyzed in six randomly chosen meningiomas, brain and dura mater using geNorm, NormFinder, Bestkeeper-1 software and the comparative ΔCt method.
Pfister Christina, Tatabiga Marcos S, Roser Florian   +2 more
doaj   +1 more source

Selection of reference genes for transcriptional analysis of edible tubers of potato (Solanum tuberosum L.). [PDF]

PLoS ONE, 2015
Potato (Solanum tuberosum) yield has increased dramatically over the last 50 years and this has been achieved by a combination of improved agronomy and biotechnology efforts.
Roberta Fogliatto Mariot, Luisa Abruzzi de Oliveira, Marleen M Voorhuijzen, Martijn Staats, Ronald C B Hutten, Jeroen P Van Dijk, Esther Kok, Jeverson Frazzon   +7 more
doaj   +1 more source

Selection of suitable reference genes for gene expression studies in HMC3 cell line by quantitative real-time RT-PCR

Scientific Reports
Microglia represent the primary immune defense system within the central nervous system and play a role in the inflammatory processes occurring in numerous disorders, such as Parkinson’s disease (PD).
Martina Fazzina, Matteo Bergonzoni, F. Massenzio, Barbara Monti, F. Frabetti, R. Casadei   +5 more
semanticscholar   +1 more source

Characterization of the Lipid Profile of Immature and In Vitro Matured Cat Oocytes

Molecular Reproduction and Development, Volume 92, Issue 8, August 2025.
ABSTRACT Forskolin (FSK), l‐carnitine (LC), and Conjugated Linoleic Acid (CLA) are lipid modulators that reduce cellular lipid content. This study characterizes the lipid profile and mRNA content of immature and in vitro matured cat oocytes with or without a MIX of these modulators. Ovaries were collected, COC retrieved, and allocated into three groups:
Erlandia M. Vasconcelos, Thais G. de Oliveira, Ribrio I. T. P. Batista, Paulo S. C. Rangel, Georgia C. Atella, Gabriela R. Leal, Joanna M. G. Souza‐Fabjan   +6 more
wiley   +1 more source

Influence of heat stress on reference genes stability in heart and liver of two chickens genotypes.

PLoS ONE, 2020
INTRODUCTION:Real-time polymerase chain reaction (RT-qPCR) is an important tool for analyzing gene expression. However, before analyzing the expression of target genes, it is crucial to normalize the reference genes, in order to find the most stable gene
Juliana Gracielle Gonzaga Gromboni, Haniel Cedraz de Oliveira, Daniele Botelho Diniz Marques, Antônio Amândio Pinto Garcia Junior, Ronaldo Vasconcelos Farias Filho, Caio Fernando Gromboni, Teillor Machado Souza, Amauri Arias Wenceslau   +7 more
doaj   +1 more source

Validation of reference genes aiming accurate normalization of qRT-PCR data in Dendrocalamus latiflorus Munro. [PDF]

PLoS ONE, 2014
BACKGROUND: Dendrocalamus latiflorus Munro distributes widely in subtropical areas and plays vital roles as valuable natural resources. The transcriptome sequencing for D.
Mingying Liu, Jing Jiang, Xiaojiao Han, Guirong Qiao, Renying Zhuo   +4 more
doaj   +1 more source

Screening of Reference miRNA of Different Early- and Late-Flowering Tree Peony Varieties

Plants, 2023
miRNA plays an important role in plant growth and development and in response to various stresses. Quantitative real-time PCR (qRT-PCR) technology is often used to detect the expression level of miRNAs and genes by comparing with reference genes.
Jiajia Shen, Xiaohui Wang, Yuying Li, Lili Guo, Xiaogai Hou   +4 more
doaj   +1 more source

Selection and Validation of Reference Genes in Sudan Grass (Sorghum sudanense (Piper) Stapf) under Various Abiotic Stresses by qRT-PCR

Genes
Quantitative reverse transcription PCR (qRT-PCR) can screen applicable reference genes of species, and reference genes can be used to reduce experimental errors.
Fangyan Wang, Peng Li, Qiuxu Liu, G. Nie, Yongqun Zhu, Xinquan Zhang   +5 more
semanticscholar   +1 more source

Selection of Reference Genes for Quantitative Real-Time PCR in Bamboo (Phyllostachys edulis)

PLoS ONE, 2013
Background The Moso bamboo (Phyllostachys edulis) is one of the most important forestry resources and plays essential ecological roles in southern China.
Chunjie Fan, Jinmin Ma, Qirong Guo, Xiaotie Li, Hui Wang, Mengzhu Lu   +5 more
semanticscholar   +1 more source

Selection and Validation of Reference Genes for RT-qPCR Analysis of Gene Expression in Nicotiana benthamiana upon Single Infections by 11 Positive-Sense Single-Stranded RNA Viruses from Four Genera

Plants, 2023
Quantitative real-time PCR (RT-qPCR) is a widely used method for studying alterations in gene expression upon infections caused by diverse pathogens such as viruses.
Ge Zhang, Zhuo Zhang, Q. Wan, Huijie Zhou, Mengting Jiao, Hongying Zheng, Yuwen Lu, Shaofei Rao, Guanwei Wu, Jianping Chen, Fei Yan, Jiejun Peng, Jian Wu   +12 more
semanticscholar   +1 more source