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Assessing Antibody Specificity in Human Serum Using Deep Sequence-Coupled Biopanning
2019Affinity selection using phage-display technologies is a powerful tool for identifying the peptide epitopes of monoclonal antibodies. Coupling affinity selection with deep sequencing technologies allows for the broad assessment of selectant populations.
Kathryn M, Frietze +4 more
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Langmuir, 2018
Biopanning refers to the processes of screening peptides with a high affinity to a target material. Microfluidic biopanning has advantages compared to conventional biopanning which requires large amounts of the target material and involves inefficient multiple pipetting steps to remove nonspecific or low-affinity peptides.
Dong Jae Lee +6 more
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Biopanning refers to the processes of screening peptides with a high affinity to a target material. Microfluidic biopanning has advantages compared to conventional biopanning which requires large amounts of the target material and involves inefficient multiple pipetting steps to remove nonspecific or low-affinity peptides.
Dong Jae Lee +6 more
openaire +2 more sources
Chapter Seven Biopanning by activation tagging
2002Publisher Summary This chapter focuses on biopanning by activation tagging. Extensive gene duplication in plants and the potential of functional redundancy among homologous sequences represent a hurdle in understanding physiological and biochemical functions of a vast number of plant genes by using conventional loss-of-function mutant screen ...
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Journal of Theoretical Biology, 1995
A mathematical model is presented for the process of selection of peptides for binding to a target. The affinity enrichment process commonly known as biopanning relies on subjecting a library of peptides on filamentous phage to a selection for binding to the target immobilized on solid support or in solution.
W, Mandecki, Y C, Chen, N, Grihalde
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A mathematical model is presented for the process of selection of peptides for binding to a target. The affinity enrichment process commonly known as biopanning relies on subjecting a library of peptides on filamentous phage to a selection for binding to the target immobilized on solid support or in solution.
W, Mandecki, Y C, Chen, N, Grihalde
openaire +2 more sources
Peptide‐Based Recognition Agents of Histamine: A Biopanning Approach with Enhanced Specificity
ChemBioChemAbstractHistamine is a biogenic amine that poses a potential threat to public health due to its toxicological effects. In this study, we identified histamine‐binding peptides by screening a random 12‐mer peptide library, employing a novel biopanning approach that excluded histidine‐binding sequences in the final round. This additional step enhanced the
Hafize Öz, Fahriye Ceyda Dudak
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A High-Throughput Magnetic Nanoparticle-Based Semi-Automated Antibody Phage Display Biopanning
2018Panning is a common process used for antibody selection from phage antibody libraries. There are several methods developed for a similar purpose, namely streptavidin mass spectrometry immunoassay (MSIA™) Disposable Automation Research Tips, magnetic beads, polystyrene immunotubes, and microtiter plate.
Ch'ng, A. +3 more
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Phage Display Technology: Affinity Selection by Biopanning
2000G K, Ehrlich, W, Berthold, P, Bailon
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