RNF219 regulates CCR4-NOT function in mRNA translation and deadenylation [PDF]
Scientific Reports, 2022 Post-transcriptional regulatory mechanisms play a role in many biological contexts through the control of mRNA degradation, translation and localization.
Aude Guénolé +7 more
doaj +7 more sources
The CCR4–NOT complex maintains liver homeostasis through mRNA deadenylation [PDF]
Life Science Alliance, 2020 The CCR4–NOT complex maintains liver homeostasis by fine-tuning levels of mRNAs, including those for transcription factors, cell cycle regulators, DNA damage response proteins, and liver function–related molecules.
Akinori Takahashi +12 more
doaj +3 more sources
The architecture of the Schizosaccharomyces pombe CCR4-NOT complex [PDF]
Nature Communications, 2016 CCR4-NOT is a protein complex involved in a variety of important genetic processes. Here, the authors report the mid-resolution structure of this complex, and model the positions and contacts between the subunits, providing structural support for the ...
Marta Ukleja +7 more
doaj +4 more sources
Heterogeneity and complexity within the nuclease module of the Ccr4-Not complex [PDF]
Frontiers in Genetics, 2013 The shortening of the poly(A) tail of cytoplasmic mRNA (deadenylation) is a pivotal step in the regulation of gene expression in eukaryotic cells. Deadenylation impacts on both regulated mRNA decay as well as the rate of mRNA translation.
Gerlof Sebastiaan Winkler +1 more
doaj +3 more sources
Ccr4–Not complex reduces transcription efficiency in heterochromatin [PDF]
Nucleic Acids Research, 2021 Abstract Heterochromatic silencing is thought to occur through a combination of transcriptional silencing and RNA degradation, but the relative contribution of each pathway is not known. In this study, we analyzed RNA Polymerase II (RNA Pol II) occupancy and levels of nascent and steady-state RNA in different mutants of ...
Pablo Monteagudo-Mesas +5 more
openaire +2 more sources
Human Ccr4–Not complexes contain variable deadenylase subunits [PDF]
Biochemical Journal, 2009 The Ccr4–Not complex is evolutionarily conserved and important for regulation of mRNA synthesis and decay. The composition of the yeast complex has been well described. Orthologues of the yeast Ccr4–Not components have been identified in human cells including multiple subunits with mRNA deadenylase activity.
Lau, N.C. +6 more
openaire +3 more sources
The CCR4–NOT Deadenylase Complex Maintains Adipocyte Identity [PDF]
International Journal of Molecular Sciences, 2019 Shortening of poly(A) tails triggers mRNA degradation; hence, mRNA deadenylation regulates many biological events. In the present study, we generated mice lacking the Cnot1 gene, which encodes an essential scaffold subunit of the CCR4–NOT deadenylase complex in adipose tissues (Cnot1-AKO mice) and we examined the role of CCR4–NOT in adipocyte function.
Akinori Takahashi +7 more
openaire +2 more sources
Regulation of eukaryotic mRNA deadenylation and degradation by the Ccr4-Not complex
Frontiers in Cell and Developmental Biology, 2023 Accurate and precise regulation of gene expression programmes in eukaryotes involves the coordinated control of transcription, mRNA stability and translation.
Lorenzo Pavanello +2 more
doaj +1 more source
Cytoplasmic deadenylase Ccr4 is required for translational repression of LRG1 mRNA in the stationary phase [PDF]
, 2017 Ccr4 is a major cytoplasmic deadenylase involved in mRNA poly(A) tail shortening in Saccharomyces cerevisiae. We have previously shown that Ccr4 negatively regulates expression of LRG1 mRNA encoding a GTPase-activating protein for the small GTPase Rho1 ...
Duy Duong Long +4 more
core +10 more sources
Hyperphosphorylation amplifies UPF1 activity to resolve stalls in nonsense-mediated mRNA decay. [PDF]
, 2016 Many gene expression factors contain repetitive phosphorylation sites for single kinases, but the functional significance is poorly understood. Here we present evidence for hyperphosphorylation as a mechanism allowing UPF1, the central factor in nonsense-
Durand, Sébastien +2 more
core +10 more sources