Results 21 to 30 of about 284,909 (310)
Molecular Plant-Microbe Interactions, 2018 Yeast-two-hybrid (Y2H) cDNA library screening is a valuable tool to uncover protein-protein interactions and represents a widely used method to investigate protein function. However, low transcript representation in cDNA libraries limits the depth of the
Cleverson Carlos Matiolli, Maeli Melotto
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BioTechniques, 2001 Here, we describe the application of a RecA-based cloning technology to generate full-length cDNA libraries enriched for genes that are differentially expressed between tumor and normal tissue samples.
B. Zhumabayeva +4 more
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High-throughput plasmid cDNA library screening [PDF]
Nature Protocols, 2006 Libraries of cDNA clones are valuable resources for analyzing the expression, structure and regulation of genes, and for studying protein functions and interactions. Full-length cDNA clones provide information about intron and exon structures, splice junctions, and 5' and 3' untranslated regions (UTRs).
Wan, Kenneth H. +7 more
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Development of a Yeast Trihybrid Screen Using Stable Yeast Strains and Regulated Protein Expression
BioTechniques, 1998 We describe a yeast trihybrid system that facilitates rapid screening of cDNA libraries. Novel yeast vectors were developed that direct integration of cDNA encoding the bait and third protein component into the yeast chromosome.
K.J. Fuller +4 more
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Scientific Reports, 2022 The full-length double-strand cDNA sequencing, one of the RNA-Seq methods, is a powerful method used to investigate the transcriptome status of a gene of interest, such as its transcription level and alternative splicing variants.
Hiroki Ura, Sumihito Togi, Yo Niida
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Display of a Maize cDNA library on baculovirus infected insect cells
BMC Biotechnology, 2008 Background Maize is a good model system for cereal crop genetics and development because of its rich genetic heritage and well-characterized morphology. The sequencing of its genome is well advanced, and new technologies for efficient proteomic analysis ...
Jones Ian M +4 more
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Tobacco Genes Induced by the Bacterial Effector Protein AvrPto
Molecular Plant-Microbe Interactions, 2004 The type III effector protein AvrPto acts as a virulence factor in susceptible plants lacking a cognate resistance gene but triggers hypersensitive response and disease resistance in tomato plants carrying the Pto gene or in tobacco plants carrying an ...
Venkatappa K. Thara +6 more
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Neurobiology of Disease, 2001 Due to brain tissue heterogeneity, the molecular genetic profile of any neurotransmitter-specific neuronal subtype is unknown. The purpose of this study was to purify a population of dopamine neurons, construct a cDNA library, and generate an initial ...
Tanya Barrett +19 more
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Assessment of Ion S5 NGS protocol for SARS-CoV-2 genome sequencing
Genetics & Applications, 2021 Monitoring of the lineages SARS-CoV-2 is equally important in a fight against COVID-19 epidemics, as is regular RT - PCR testing. Ion AmpliSeq Library kit plus is a robust and validated protocol for library preparation, but certain optimizations for
Dino Pećar +3 more
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Biologia Plantarum, 2013 Chili pepper (Capsicum annuum L.) cv. Árbol and Uvilla fruits differing in anthocyanin contents were analyzed to characterize the accumulation patterns.
C. Aza-González +4 more
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