Results 61 to 70 of about 400,889 (317)

High-resolution mapping of single cells in spatial context

Nature Communications
Spatially resolved transcriptomic technologies have emerged as pivotal tools for elucidating molecular regulation and cellular interplay within the intricate tissue microenvironment, but hampered by insufficient gene recovery or challenges in achieving ...
Jincan Ke, Jian Xu, Jia Liu, Yumeng Yang, Chenkai Guo, Bingbing Xie, Guizhong Cui, Guangdun Peng, Shengbao Suo   +8 more
doaj   +1 more source

Single-cell analysis reveals T cell dysfunction driven by macrophages and differential expression of transposable elements in severe COVID-19 patients

Heliyon
The vastly spreading COVID-19 pneumonia is caused by SARS-CoV-2. Lymphopenia and cytokine levels are tightly associated with disease severity. However, virus-induced immune dysregulation at cellular and molecular levels remains largely undefined.
Airu Zhu, Liang Zhou, Zhao Chen, Dongdong Liu, Huijian Feng, Baomei Cai, Xinwen Chen, Jincun Zhao, Jingxian Zhao, Jiekai Chen, Manshu Li, Jiangping He   +11 more
doaj   +1 more source

Aggressive prostate cancer is associated with pericyte dysfunction

Molecular Oncology, EarlyView.
Tumor‐produced TGF‐β drives pericyte dysfunction in prostate cancer. This dysfunction is characterized by downregulation of some canonical pericyte markers (i.e., DES, CSPG4, and ACTA2) while maintaining the expression of others (i.e., PDGFRB, NOTCH3, and RGS5).
Anabel Martinez‐Romero, Ane Martinez‐Larrinaga, Joaquim Grego‐Bessa, Saioa Garcia‐Longarte, Hielke van Splunder, Ianire Astobiza, Amaia Ercilla, Laura Bozal‐Basterra, Isabel Mendizabal, Pilar Villacampa, Arkaitz Carracedo, Mariona Graupera   +11 more
wiley   +1 more source

Generation of a PPM1A-deficient human induced pluripotent stem cell line using CRISPR-Cas9 technology

Stem Cell Research
PPM1A is a member of the serine/threonine protein phosphatase family. It can bind to a variety of proteins to dephosphorylate them, and extensively regulates many life activities such as cell growth, cell stress, immune response, and tumor formation ...
Xinrui Guo, Kui Zhao, Yanqi Zhang, Tiancheng Zhou, Guangjin Pan   +4 more
doaj   +1 more source

Plectus - a stepping stone in embryonic cell lineage evolution of nematodes

EvoDevo, 2012
Background Recent studies have challenged the widespread view that the pattern of embryogenesis found in Caenorhabditis elegans (clade 9) is characteristic of nematodes in general.
Schulze Jens, Houthoofd Wouter, Uenk Jana, Vangestel Sandra, Schierenberg Einhard   +4 more
doaj   +1 more source

Synergy of single-cell sequencing analyses and in vivo lineage-tracing approaches: A new opportunity for stem cell biology [PDF]

, 2022
Yuki Matsushita, Wanida Ono, Noriaki Ono
openalex   +1 more source

In vitro models of cancer‐associated fibroblast heterogeneity uncover subtype‐specific effects of CRISPR perturbations

Molecular Oncology, EarlyView.
Development of therapies targeting cancer‐associated fibroblasts (CAFs) necessitates preclinical model systems that faithfully represent CAF–tumor biology. We established an in vitro coculture system of patient‐derived pancreatic CAFs and tumor cell lines and demonstrated its recapitulation of primary CAF–tumor biology with single‐cell transcriptomics ...
Elysia Saputra, Shamsudheen Karuthedath Vellarikkal, Lixia Li, Hong Sun, Khoa Nguyen, Amber Montano, Suchitra Natarajan, Federica Piccioni, Alex Michael Tamburino, Xin Yu, Aleksandra Katarzyna Olow   +10 more
wiley   +1 more source

Cis‐regulatory and long noncoding RNA alterations in breast cancer – current insights, biomarker utility, and the critical need for functional validation

Molecular Oncology, EarlyView.
The noncoding region of the genome plays a key role in regulating gene expression, and mutations within these regions are capable of altering it. Researchers have identified multiple functional noncoding mutations associated with increased cancer risk in the genome of breast cancer patients.
Arnau Cuy Saqués, Aracele Martinez‐Mendez, John Crown, Alex Eustace   +3 more
wiley   +1 more source

Dual-nuclease single-cell lineage tracing by Cas9 and Cas12a

Cell Reports
Summary: Single-cell lineage tracing based on CRISPR-Cas9 gene editing enables the simultaneous linkage of cell states and lineage history at a high resolution.
Cheng Chen, Yuanxin Liao, Miao Zhu, Li Wang, Xinran Yu, Meishi Li, Guangdun Peng   +6 more
doaj   +1 more source

Cell lineage: Compartments and Capricious [PDF]

Current Biology, 2001
Until recently, little was known about the mechanisms that prevent cell migration across compartment boundaries in Drosophila. A new report suggests that the lineage restriction between the dorsal and ventral compartments of the developing wing relies in part on the transmembrane proteins, Capricious and Tartan.
openaire   +2 more sources