Results 1 to 10 of about 4,791 (192)

Insights into lignocellulose degradation: comparative genomics of anaerobic and cellulolytic Ruminiclostridium-type species [PDF]

open access: yesFrontiers in Microbiology, 2023
Mesophilic, anaerobic, and cellulolytic Ruminiclostridium-type bacterial species can secrete an extracellular, multi-enzyme machinery cellulosome, which efficiently degrades cellulose.
Mengcheng You   +9 more
doaj   +2 more sources

Designing chimeric enzymes inspired by fungal cellulosomes

open access: yesSynthetic and Systems Biotechnology, 2020
Cellulosomes are synthesized by anaerobic bacteria and fungi to degrade lignocellulose via synergistic action of multiple enzymes fused to a protein scaffold.
Sean P. Gilmore   +4 more
doaj   +3 more sources

Deciphering Cellodextrin and Glucose Uptake in Clostridium thermocellum

open access: yesmBio, 2022
Sugar uptake is of great significance in industrially relevant microorganisms. Clostridium thermocellum has extensive potential in lignocellulose biorefineries as an environmentally prominent, thermophilic, cellulolytic bacterium.
Fei Yan   +10 more
doaj   +1 more source

Cellulosomes from Mesophilic Bacteria [PDF]

open access: yesJournal of Bacteriology, 2003
Plant cell wall-degrading enzymes have become increasingly important, since the development of efficient biomass degradation methods and the conversion of sugars to valuable products such as butanol and amino acids and utilizable forms of energy such as ethanol and methane could lead to less dependence on imported petroleum as a fuel and chemical ...
Roy H, Doi   +4 more
openaire   +2 more sources

Enzymatic Characterization of Unused Biomass Degradation Using the Clostridium cellulovorans Cellulosome

open access: yesMicroorganisms, 2022
The cellulolytic system of Clostridium cellulovorans mainly consisting of a cellulosome that synergistically collaborates with non-complexed enzymes was investigated using cellulosic biomass.
Mohamed Yahia Eljonaid   +3 more
doaj   +1 more source

Combinatorial assembly and optimisation of designer cellulosomes: a galactomannan case study

open access: yesBiotechnology for Biofuels and Bioproducts, 2022
Background Designer cellulosomes are self-assembled chimeric enzyme complexes that can be used to improve lignocellulosic biomass degradation. They are composed of a synthetic multimodular backbone protein, termed the scaffoldin, and a range of different
Julie Vanderstraeten   +8 more
doaj   +1 more source

Clostridium clariflavum: Key Cellulosome Players Are Revealed by Proteomic Analysis

open access: yesmBio, 2015
Clostridium clariflavum is an anaerobic, cellulosome-forming thermophile, containing in its genome genes for a large number of cellulosomal enzyme and a complex scaffoldin system.
Lior Artzi   +4 more
doaj   +1 more source

Improving the Synthesis Efficiency of Amino Acids Such as L-Lysine by Assembling Artificial Cellulosome Elements Dockerin Protein In Vivo

open access: yesFermentation, 2022
Cellulosome is a highly efficient multi-enzyme self-assembly system and is found on the extracellular surface or in the free environment of microorganisms. However, with a lack of Ca2+ in vivo, cellulosome assembly is challenging.
Nan Li   +9 more
doaj   +1 more source

Current challenges in designer cellulosome engineering

open access: yesApplied Microbiology and Biotechnology, 2023
Designer cellulosomes (DCs) are engineered multi-enzyme complexes, comprising carbohydrate-active enzymes attached to a common backbone, the scaffoldin, via high-affinity cohesin-dockerin interactions. The use of DCs in the degradation of renewable biomass polymers is a promising approach for biorefineries. Indeed, DCs have shown significant hydrolytic
Babette Lamote   +5 more
openaire   +3 more sources

Nanoscale Engineering of Designer Cellulosomes

open access: yesAdvanced Materials, 2016
Biocatalysts showcase the upper limit obtainable for high‐speed molecular processing and transformation. Efforts to engineer functionality in synthetic nanostructured materials are guided by the increasing knowledge of evolving architectures, which enable controlled molecular motion and precise molecular recognition.
Gunnoo M.   +12 more
  +7 more sources

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