Enhancement of cellulosome-mediated deconstruction of cellulose by improving enzyme thermostability [PDF]
, 2016 Background: The concerted action of three complementary cellulases from Clostridium thermocellum, engineered to be stable at elevated temperatures, was examined on a cellulosic substrate and compared to that of the wild-type enzymes. Exoglucanase Cel48S
Arnold, Frances H. +9 more
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Comparison of Family 9 Cellulases from Mesophilic and Thermophilic Bacteria [PDF]
, 2011 Cellulases containing a family 9 catalytic domain and a family 3c cellulose binding module (CBM3c) are important components of bacterial cellulolytic systems.
Arnold, Frances H. +4 more
core +2 more sources
Predicting Selective RNA Processing and Stabilization Operons in Clostridium spp.
Frontiers in Microbiology, 2021 In selective RNA processing and stabilization (SRPS) operons, stem–loops (SLs) located at the 3′-UTR region of selected genes can control the stability of the corresponding transcripts and determine the stoichiometry of the operon. Here, for such operons,
Yogendra Bhaskar +5 more
doaj +1 more source
Comparison of the mesophilic cellulosome‐producing Clostridium cellulovorans genome with other cellulosome‐related clostridial genomes [PDF]
Microbial Biotechnology, 2010 Summary Clostridium cellulovorans , an anaerobic and mesophilic bacterium, degrades native substrates in soft biomass such as corn fibre and rice straw efficiently by producing an extracellular enzyme complex called the cellulosome. Recently, we have reported the whole‐genome sequence of
Tamaru, Yutaka +6 more
openaire +4 more sources
Pan-Cellulosomics of Mesophilic Clostridia: Variations on a Theme
Microorganisms, 2017 The bacterial cellulosome is an extracellular, multi-enzyme machinery, which efficiently depolymerizes plant biomass by degrading plant cell wall polysaccharides.
Bareket Dassa +6 more
doaj +1 more source
Frontiers in Microbiology, 2022 As part of free cellulases or scaffolding proteins in cellulosomes, the hydrophilic non-catalytic X2 module is widely distributed in cellulolytic Clostridia or other Firmicutes bacteria. Previous biochemical studies suggest that X2 modules might increase
Xuanyu Tao +8 more
doaj +1 more source
Driving biomass breakdown through engineered cellulosomes [PDF]
Bioengineered, 2015 Extraction of sugar is the rate-limiting step in converting unpretreated biomass into value-added products through microbial fermentation. Both anaerobic fungi and anaerobic bacteria have evolved to produce large multi-cellulase complexes referred to as cellulosomes, which are powerful machines for biomass deconstruction.
Gilmore, Sean P +2 more
openaire +2 more sources
Nanoscale resolution of microbial fiber degradation in action
eLife, 2022 The lives of microbes unfold at the micron scale, and their molecular machineries operate at the nanoscale. Their study at these resolutions is key toward achieving a better understanding of their ecology.
Meltem Tatli +6 more
doaj +1 more source
Synergistic interaction of the cellulosome integrating protein (CipA) from Clostridium thermocellum with a cellulosomal endoglucanase [PDF]
FEBS Letters, 1998 Activity of a cellulosomal endoglucanase (endoglucanase E; EGE) from Clostridium thermocellum against two crystalline forms of cellulose was enhanced by combination with the cellulosome integrating protein (CipA), but CipA did not enhance EGE activity against amorphous cellulose, even though it was able to bind to it.
Ciruela, Antonio +3 more
openaire +2 more sources
Glycosylation of hyperthermostable designer cellulosome components yields enhanced stability and cellulose hydrolysis [PDF]
, 2020 Biomass deconstruction remains integral for enabling second‐generation biofuel production at scale. However, several steps necessary to achieve significant solubilization of biomass, notably harsh pretreatment conditions, impose economic barriers to ...
Bayer, Edward A. +8 more
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