Results 51 to 60 of about 4,791 (192)

A synthetic biology approach for evaluating the functional contribution of designer cellulosome components to deconstruction of cellulosic substrates [PDF]

, 2013
BACKGROUND: Select cellulolytic bacteria produce multi-enzymatic cellulosome complexes that bind to the plant cell wall and catalyze its efficient degradation.
Edward A Bayer, Ehud Shapiro, Melina Shamshoum, Raphael Lamed, Tamar Unger, Tuval Ben-Yehezkel, Yael Vazana, Yair Mazor, Yoav Barak, Yoav Peleg   +9 more
core   +1 more source

Assembly of Xylanases into Designer Cellulosomes Promotes Efficient Hydrolysis of the Xylan Component of a Natural Recalcitrant Cellulosic Substrate

mBio, 2011
In nature, the complex composition and structure of the plant cell wall pose a barrier to enzymatic degradation. Nevertheless, some anaerobic bacteria have evolved for this purpose an intriguing, highly efficient multienzyme complex, the cellulosome ...
Sarah Moraïs, Yoav Barak, Yitzhak Hadar, David B. Wilson, Yuval Shoham, Raphael Lamed, Edward A. Bayer   +6 more
doaj   +1 more source

Eight Up-Coming Biotech Tools to Combat Climate Crisis

Microorganisms, 2023
Biotechnology has a high potential to substantially contribute to a low-carbon society. Several green processes are already well established, utilizing the unique capacity of living cells or their instruments.
Werner Fuchs, Lydia Rachbauer, Simon K.-M. R. Rittmann, Günther Bochmann, Doris Ribitsch, Franziska Steger   +5 more
doaj   +1 more source

Cellulosomal Scaffoldin-Like Proteins from Ruminococcus flavefaciens [PDF]

Journal of Bacteriology, 2001
ABSTRACT Two tandem cellulosome-associated genes were identified in the cellulolytic rumen bacterium, Ruminococcus flavefaciens . The deduced gene products represent multimodular scaffoldin-related proteins (termed ScaA and ScaB), both of which include several copies of explicit cellulosome signature ...
SHIYOU DING, MARCO T RINCON, RAPHAEL LAMED, JENNIFER C MARTIN, SHEILA I MCCRAE, VINCENZO AURILIA, YUVAL SHOHAM, EDWARD A BAYER, HARRY J FLINT   +8 more
openaire   +4 more sources

Early-branching gut fungi possess a large, comprehensive array of biomass-degrading enzymes [PDF]

, 2016
available in PMC 2016 November 07The fungal kingdom is the source of almost all industrial enzymes in use for lignocellulose bioprocessing. We developed a systems-level approach that integrates transcriptomic sequencing, proteomics, phenotype, and ...
Borges-Rivera, Diego Ramon, Brewer, Heather M., Gilmore, Sean P., Grigoriev, Igor V., Haitjema, Charles H., Henske, John K., Lipzen, Anna, O'Malley, Michelle A., Purvine, Samuel O., Regev, Aviv, Solomon, Kevin V., Theodorou, Michael K., Thompson, Dawn A., Wright, Aaron T.   +13 more
core   +2 more sources

Synergies between cellulosomal and non-cellulosomal bacterial cellulases complexed in designer cellulosomes

International Journal of Biological Macromolecules
The development of cellulases cocktails to efficiently solubilize cellulose for biotechnological applications is a constant demand. Cellulases exhibit diverse mode of action, operating in the free state or in multi-enzyme complexes (cellulosomes), and potentially acting synergistically for a higher efficiency.
David, Hélène, Vita, Nicolas, Pagès, Sandrine, Štravs, Petra, Berlec, Aleš, Fierobe, Henri-Pierre, Perret, Stéphanie   +6 more
openaire   +4 more sources

Simultaneous cell growth and ethanol production from cellulose by an engineered yeast consortium displaying a functional mini-cellulosome

Microbial Cell Factories, 2011
Background The recalcitrant nature of cellulosic materials and the high cost of enzymes required for efficient hydrolysis are the major impeding steps to their practical usage for ethanol production.
Madan Bhawna, Tsai Shen-Long, Goyal Garima, DaSilva Nancy A, Chen Wilfred   +4 more
doaj   +1 more source

DEVELOPMENT OF MICROORGANISMS FOR CELLULOSE-BIOFUEL CONSOLIDATED BIOPROCESSINGS: METABOLIC ENGINEERS' TRICKS

Computational and Structural Biotechnology Journal, 2012
Cellulose waste biomass is the most abundant and attractive substrate for “biorefinery strategies” that are aimed to produce high-value products (e.g.
Roberto Mazzoli
doaj   +3 more sources

Cellulosomal hemicellulases: Indispensable players for ensuring effective lignocellulose bioconversion

Green Carbon
The bioconversion of lignocellulose has attracted global attention, due to the significant potential of agricultural and forestry wastes as renewable zero-carbon resources and the urgent need for substituting fossil carbon.
Min Xiao, Ya-Jun Liu, Edward A. Bayer, Akihiko Kosugi, Qiu Cui, Yingang Feng   +5 more
doaj   +1 more source

Extending the cellulosome paradigm: the modular clostridium thermocellum cellulosomal serpin pinA is a broad-spectrum inhibitor of subtilisin-like proteases [PDF]

, 2013
Clostridium thermocellum encodes a cellulosomal, modular, and thermostable serine protease inhibitor (serpin), PinA. PinA stability but not inhibitory activity is affected by the Fn(III) and Doc(I) domains, and PinA is a broad inhibitor of subtilisin ...
Goswami, Hareshwar P., Gupta, Rajesh, Morrison, Mark, Ó Cuív, Páraic   +3 more
core   +1 more source