Results 41 to 50 of about 400,071 (226)

Europium-Labeled Synthetic C3a Protein as a Novel Fluorescent Probe for Human Complement C3a Receptor [PDF]

Bioconjugate Chemistry, 2017
Measuring ligand affinity for a G protein-coupled receptor is often a crucial step in drug discovery. It has been traditionally determined by binding putative new ligands in competition with native ligand labeled with a radioisotope of finite lifetime.
Aline Dantas de Araujo, Chongyang Wu, Kai-Chen Wu, Robert C. Reid, Thomas Durek, Junxian Lim, David P. Fairlie   +6 more
openaire   +5 more sources

Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assay

, 2021
Enzyme-linked immunosorbent assay (ELISA) enables fast and simple quantification of analytes in the pico- to nanogram range in complex samples. Here, we describe an ELISA for the detection of porcine C3a as a marker for complement activation.
Skjeflo, Espen W.,, Christiansen, Dorte,, Mollnes, Tom Eirik,, Fure, Hilde, Kristin Pettersen, Tom Eirik Mollnes, Roumenina, Lubka T., Nilsson, Per H., Fure, Hilde,, Nilsson, Per, Pettersen, Kristin, Espen W. Skjeflo, Dorte Christiansen, Skjeflo, Espen Waage, Martin Oppermann, Nilsson, Per H.,, Hilde Fure, Christiansen, Dorte, Mollnes, Tom Eirik, Skjeflo, Espen W., Oppermann, Martin, Oppermann, Martin,, Per H. Nilsson, Pettersen, Kristin,   +23 more
core   +1 more source

Efficient chemical synthesis of human complement protein C3a [PDF]

Chemical Communications, 2013
We report the total chemical synthesis of human C3a by one-pot native chemical ligation of three unprotected peptide segments, followed by efficient in vitro folding that yielded the anaphylatoxin C3a in high yield and excellent purity. Synthetic C3a was fully active and its crystal structure at 2.1 Å resolution showed 3 helices and a C-terminal turn ...
Ghassemian, Artin, Wang, Ching-I Anderson, Yau, Mei-Kwan, Reid, Robert C., Lewis, Richard J., Fairlie, David P., Alewood, Paul F., Durek, Thomas   +7 more
openaire   +5 more sources

Staphylococcus aureus proteins Sbi and Efb recruit human plasmin to degrade complement C3 and C3b [PDF]

, 2012
Upon host infection, the human pathogenic microbe Staphylococcus aureus (S. aureus) immediately faces innate immune reactions such as the activated complement system. Here, a novel innate immune evasion strategy of S. aureus is described.
Peter F. Zipfel, Christine Skerka, Kraiczy, Peter, Michael Reuter, Reuter, Michael, Skerka, Christine, Barthel, Diana, Diana Barthel, Koch, Tina K., Elsen, Jean van den, Böhm, Sascha, Zipfel, Peter F., Jean van den Elsen, Peter Kraiczy, Tina K Koch, Sascha Böhm, Peter F Zipfel, Tina K. Koch   +17 more
core   +1 more source

Antimicrobial peptides and complement in neonatal hypoxia-ischemia induced brain damage [PDF]

, 2015
Hypoxic-ischemic encephalopathy (HIE) is a clinical condition in the neonate, resulting from oxygen deprivation around the time of birth. HIE affects 1-5/1000 live births worldwide and is associated with the development of neurological deficits ...
Rocha-Ferreira, E, Eridan Rocha-Ferreira, Eridan eRocha-Ferreira, Mariya eHristova, Hristova, M, Mariya Hristova   +5 more
core   +1 more source

Reduced order modeling and analysis of the human complement system. [PDF]

PLoS ONE, 2017
Complement is an important pathway in innate immunity, inflammation, and many disease processes. However, despite its importance, there are few validated mathematical models of complement activation.
Adithya Sagar, Wei Dai, Mason Minot, Rachel LeCover, Jeffrey D Varner   +4 more
doaj   +1 more source

Complement components C3a promoted tumor recurrence after liver transplantation

, 2021
Introduction: Complement components are the keys regulators orchestrating many cellular responses including immunological responses. Increasing evidences showed complement components involved in many liver pathology.
Lo, CM, Liu, H, Man, K, Yeung, WHO, Ng, TPK   +4 more
core   +1 more source

Complement activation contributes to perioperative neurocognitive disorders in mice

Journal of Neuroinflammation, 2018
Background The complement system plays an important role in many neurological disorders. Complement modulation, including C3/C3a receptor signaling, shows promising therapeutic effects on cognition and neurodegeneration.
Chao Xiong, Jinhu Liu, Dandan Lin, Juxia Zhang, Niccolò Terrando, Anshi Wu   +5 more
doaj   +1 more source

Table_1_The association between serum complement C3a and severity in patients with community-acquired pneumonia.docx

, 2023
BackgroundA few studies found that the complement system may be involved in the onset and progression of community-acquired pneumonia (CAP). However, the role of the complement system in CAP was obscure.
Hui Zhao (7395), Ling Zheng (276125), Xue-Feng Hou (14512367), De-Xiang Xu (293524), Lin Fu (320968), Zheng Xu (162992), Chun-Mei Feng (8203425)   +6 more
core   +1 more source

Characterization of Synthetic C3a Analog Peptides on Human Eosinophils in Comparison to the Native Complement Component C3a [PDF]

The Journal of Immunology, 2000
Abstract The C3a anaphylatoxin is a potent proinflammatory mediator derived from the complement system inducing biologic effects of human eosinophils like Ca2+ transients and the activation of the respiratory burst. These findings support an important role for C3a in diseases typically associated with a peripheral blood or tissue ...
H, Petering, J, Köhl, A, Weyergraf, Y, Dulkys, D, Kimmig, R, Smolarski, A, Kapp, J, Elsner   +7 more
openaire   +2 more sources