Results 71 to 80 of about 6,459 (197)
Fluorogenic “click” reaction for labeling and detection of DNA in proliferating cells
BioTechniques, 2010 A thymidine analog, 5-ethynyl-2′-deoxyuridine (EdU), has been reported as a rapid labeling tool for direct measurement of cells in S-phase. The alkynyl group of EdU is a biologically inert group that undergoes an extremely selective reaction with azido ...
Kai Li +3 more
doaj +1 more source
Click Chemistry (CuAAC) and Detection of Tagged de novo Synthesized Proteins in Drosophila
Bio-Protocol, 2019 Copper-catalyzed azide-alkyne-cycloaddition (CuAAC), also known as ‘click chemistry’ serves as a technique for bio-orthogonal, that is, bio-compatible labeling of macromolecules including proteins or lipids.
Kathrin Marter +8 more
doaj +1 more source
Circular Photocaged mRNA for Light‐induced Late‐stage Activation of Translation
Angewandte Chemie International Edition, EarlyView.A clickable photo‐caged 5′ cap (CyCliCap) allows to make Cyclic Click Cap‐containing mRNA (CyCliCap‐mRNA) which is remarkably stable and translationally muted until irradiated, allowing for late‐stage activation of translation in cells by light. ABSTRACT mRNA is a new medical modality as vaccine and raises hopes to become a protein replacement agent ...
Bayram Terzi +6 more
wiley +1 more source
Clicked H-Shaped Arylopeptoids
MoleculesThis study presents a tentative synthesis of supported H-shaped and ladder-type compounds. If the ladders were not accessible, probably due to distance misfits between the reactive centers, a facile method for the synthesis of H-shaped N-alkylated ...
Zein El Abidine Chamas +4 more
doaj +1 more source
BMEMat, EarlyView.Mitochondria‐endoplasmic reticulum contact sites (MERCS) are areas where the mitochondria and endoplasmic reticulum closely interact. In this study, we utilize synthetic organelle glues to artificially engineer MERCS for regulating cardiomyocyte development, through which the immature and chemo‐plasticity issues of undifferentiated cells are addressed.
Wei Tang +9 more
wiley +1 more source
Chemistry – A European Journal, EarlyView.A series of structurally diverse exoglycals was synthesized using a modified Julia‐olefination approach from sugar‐derived precursors. These glycosidase transition‐state mimics were further diversified via CuAAC chemistry to yield triazole‐conjugated analogues.
Elisa Ospanow +2 more
wiley +1 more source
Synthesis of Porphyrin and Bacteriochlorin Glycoconjugates through CuAAC Reaction Tuning
European Journal of Organic Chemistry, 2019 Rapid and reproducible access to a series of unique porphyrin and bacteriochlorin glycoconjugates, including meso‐glycosylated porphyrins and bacteriochlorins, and beta‐glycosylated porphyrins, using copper‐catalyzed azide–alkyne 1,3‐dipolar cycloaddition (CuAAC) is reported for the first time.
Matthew C. Bennion +13 more
openaire +3 more sources
Synthetic Strategies for Activity‐Based Probes to Decode Ubiquitin‐Like Modifiers
Chemistry – A European Journal, EarlyView.ABSTRACT Ubiquitin‐like proteins (Ubls) such as SUMO, NEDD8, ISG15, URM1, UFM1, FAT10, ATG8/ATG12, and FUBI are essential regulators of cellular homeostasis, controlling processes from protein stability and trafficking to immune signaling and autophagy.
Saibal Chanda +5 more
wiley +1 more source
Safirinium Fluorescent “Click” Molecular Probes: Synthesis, CuAAC Reactions, and Microscopic Imaging
MoleculesFluorescent labeling utilizing Cu(I)-catalyzed azide–alkyne cycloaddition reactions (CuAAC) is among the leading applications of the “click” chemistry strategy.
Patryk Kasza +10 more
doaj +1 more source
Synthesis and Reactivity of 3‐Aminosydnones in Cycloaddition Reactions With Alkynes
Chemistry – A European Journal, EarlyView.The synthesis, functionalization, and reactivity as dipoles of 3‐aminosydnones have been investigated. These neglected compounds undergo smooth cycloaddition reactions with strained alkynes, leading to 1‐aminopyrazoles. ABSTRACT We present in this article the synthesis, functionalization, and properties of 3‐aminosydnones, a forgotten class of ...
Apolline Dominic +7 more
wiley +1 more source