Results 61 to 70 of about 1,652 (193)

Protein Structure Determination by Racemic and Quasi‐Racemic X‐Ray Crystallography

ChemBioChem, Volume 27, Issue 4, 25 February 2026.
Racemic mixtures of protein enantiomers facilitate formation of diffraction‐quality crystals in centrosymmetric space groups that have quantized phases which simplify structure solution. Quasi‐racemic mixtures of a protein and its mirror image isomorph similarly facilitate formation of highly ordered crystals.
Stephen B. H. Kent
wiley   +1 more source

Cyclotide Interactions with the Nematode External Surface [PDF]

Antimicrobial Agents and Chemotherapy, 2010
ABSTRACTCyclotides are a large family of cyclic cystine knot-containing plant peptides that have anthelminthic activities againstHaemonchus contortusandTrichostrongylus colubriformis, two important gastrointestinal nematodes of sheep. In this study, we investigated the interaction of the prototypic cyclotide kalata B1 with the external surface ofH ...
Colgrave, Michelle L., Huang, Yen-Hua, Craik, David J., Kotze, Andrew C.   +3 more
openaire   +5 more sources

Engineered biosynthesis of cyclotides [PDF]

New Zealand Journal of Botany, 2020
A system based on cyanobacterial split inteins, SICLOPPs (Split Intein Circular Ligation of Proteins and Peptides), has been used to synthesise a small natively cyclic plant protein, kalata B1, and cyclised versions of the natively linear therapeutic peptides ziconotide and leconotide.
Thomas N. G. Handley, Hyon-Xhi Tan, Malcolm T. Rutledge, Hans Henning Brewitz, Joel D. A. Tyndall, Torsten Kleffmann, Margi I. Butler, Russell T. M. Poulter, Sigurd M. Wilbanks   +8 more
openaire   +4 more sources

Protein engineering strategies to optimise recombinant product synthesis and accumulation in Nicotiana benthamiana

Plant Biotechnology Journal, Volume 24, Issue 1, Page 31-43, January 2026.
Summary In plant molecular farming, recombinant products include traditional protein targets, such as antibodies, antigens, and enzymes, but also increasingly refers to high‐value peptides, and small molecules produced by heterologous enzymatic pathways.
Maxim D. Harding, Frank Sainsbury
wiley   +1 more source

Repositioning Antimicrobial Peptides Against WHO‐Priority Fungi

Advanced Science, Volume 12, Issue 37, October 6, 2025.
The growing burden of drug‐resistant fungal infections, driven by pathogens such as Candida auris, Cryptococcus neoformans, and Aspergillus fumigatus, underscores the urgent need for novel antifungal therapies. This review explores antimicrobial peptides as promising agents with membrane‐disruptive activity, immunomodulatory properties, and delivery ...
Cesar Augusto Roque‐Borda, Kaila Petronila Medina‐Alarcón, João Paulo Soler Gonçalves Pereira, Thais Cristina dos Anjos Sevilhano, Brigitte Aguilar‐Morón, Fernando Díaz‐Cárdenas, Lucas Silva da Cruz, Francisco Humberto Xavier‐Júnior, Eduardo Festozo Vicente, João Perdigão, Beatriz G. de la Torre, Fernando Albericio, Fernando Rogério Pavan   +12 more
wiley   +1 more source

Identification of candidates for cyclotide biosynthesis and cyclisation by expressed sequence tag analysis of Oldenlandia affinis

BMC Genomics, 2010
Background Cyclotides are a family of circular peptides that exhibit a range of biological activities, including anti-bacterial, cytotoxic, anti-HIV activities, and are proposed to function in plant defence.
Suda Jan, Kaas Quentin, McCallum Emily J, Qin Qiaoping, Saska Ivana, Craik David J, Mylne Joshua S   +6 more
doaj   +1 more source

Proposed EU NGT legislation in light of plant genetic variation

Plant Biotechnology Journal, Volume 23, Issue 10, Page 4261-4270, October 2025.
Summary The European Commission (EC) proposal for New Genomic Techniques (NGTs) of July 2023 specifies that Category 1 NGT (NGT1) plants, which are considered equivalent to conventional plants, that is those obtainable by conventional plant breeding or mutagenesis, may differ from the recipient or parental plant by no more than 20 insertions, which ...
Alan H. Schulman, Frank Hartung, Marinus J.M. Smulders, Jens F. Sundström, Ralf Wilhelm, Odd Arne Rognli, Karin Metzlaff   +6 more
wiley   +1 more source

Dynamic scenario of membrane binding process of kalata b1. [PDF]

PLoS ONE, 2014
Kalata B1 (kB1), a cyclotide that has been used in medical applications, displays cytotoxicity related to membrane binding and oligomerization. Our molecular dynamics simulation results demonstrate that Trp19 in loop 5 of both monomeric and tetrameric ...
Wanapinun Nawae, Supa Hannongbua, Marasri Ruengjitchatchawalya   +2 more
doaj   +1 more source

Exploring Plant α‐Amylase Inhibitors: Mechanisms and Potential Application for Insect Pest Control

Biotechnology Journal, Volume 20, Issue 8, August 2025.
Graphical Abstract and Lay Summary In this study, we address the importance of α‐amylases in insect pests, the possible biochemical role of α‐amylase inhibitors in plant resistance to insect pests, the molecular relationships between plant α‐amylase inhibitors and insect α‐amylases, and the biotechnological potential of proteinaceous α‐amylase ...
Marcos Fernando Basso, Arnubio Valencia‐Jiménez, Fabrizio Lo Celso, Isabel Rodrigues Gerhardt, Thomas Joseph V. Higgins, Maria Fatima Grossi‐de‐Sa   +5 more
wiley   +1 more source

Cyclotides prediction in Leptopetalum biflorum based on de novo transcriptome assembly and annotation

Journal of Holistic Integrative Pharmacy
Objective: There is a scarcity of transcriptome sequencing data available for the Leptopetalum biflorum, and numerous cyclotides remain undiscovered.
Xi Liu, Linlin Cai, Zhiming Zhou, Peiming Huang, Zhonglu Ren   +4 more
doaj   +1 more source