Results 41 to 50 of about 5,771 (244)

Human μ-calpain: Simple isolation from erythrocytes and characterization of autolysis fragments [PDF]

, 2001
Heterodimeric μ-calpain, consisting of the large (80 kDa) and the small (30 kDa) subunit, was isolated and purified from human erythrocytes by a highly reproducible four-step purification procedure.
Crawford C., Croall D. E., Graham-Siegenthaler K., Guroff G., Kitahara A., McClelland P., Nishimura T., Sasaki T., Sorimachi H., Sorimachi H., Sorimachi H., Thompson V.F., Yoshimura N., Zimmermann U.J.P.   +13 more
core   +1 more source

Differential Effect of Novel Plant Cystatins on the Adhesive Behaviour of Normal and Cancer Breast Cells [PDF]

International Journal Bioautomation
In the present work, we have investigated a novel recombinant cystatin dgECP1 and its mutant form, dgECP1m1, focused on their impact on the adhesive behaviour of two breast cell lines: the cancerous, MDA-MB-231, and the normal, MCF-10A.
Kamelia Hristova-Panusheva, Milena Keremidarska-Markova, Natalia Krasteva   +2 more
doaj   +1 more source

Small Extracellular Vesicles from Neural Cells: Physiological and Pathological Roles, and Potential in Neurodegenerative Therapy

Advanced Healthcare Materials, EarlyView.
Neural cell–derived small extracellular vesicles (sEVs) are emerging as pivotal mediators in neurodegenerative diseases, exerting both pathogenic and therapeutic functions. This review synthesizes current evidence on how sEVs from distinct neural cell types regulate neurodegeneration, neuroprotection, biomarker discovery, and targeted drug delivery ...
Muhammad Waqas Salim, Wei Zhang, Lyndsey Collins‐Praino, Yuling Wang, Andrew Care   +4 more
wiley   +1 more source

Unraveling the Molecular Mechanisms Underlying Spontaneous Multipolar Mitosis Through CIN‐seq

Advanced Science, EarlyView.
Multipolar mitosis, a hallmark of chromosomal instability (CIN), drives tumor heterogeneity but is challenging to study in live cells. Using CIN‐seq, a single‐cell multiomics method, we profiled rare CIN events and identified mechanisms associated with viable multipolar mitosis, including PTEN attenuation, Rho GTPase‐driven cytokinesis failure, and ...
Pin‐Rui Su, Ting‐Chun Chou, Maria Teresa López‐Cascales, Junfeng Huang, Tsai‐Ying Chen, Sjoukje van Wieren, Chan Li, Cecile Beerens, Li You, Jelle Storteboom, Miao‐Ping Chien   +10 more
wiley   +1 more source

TcCYPR04, a Cacao Papain-Like Cysteine-Protease Detected in Senescent and Necrotic Tissues Interacts with a Cystatin TcCYS4. [PDF]

PLoS ONE, 2015
The interaction amongst papain-like cysteine-proteases (PLCP) and their substrates and inhibitors, such as cystatins, can be perceived as part of the molecular battlefield in plant-pathogen interaction.
Thyago Hermylly Santana Cardoso, Ana Camila Oliveira Freitas, Bruno Silva Andrade, Aurizangela Oliveira de Sousa, André da Silva Santiago, Daniela Martins Koop, Karina Peres Gramacho, Fátima Cerqueira Alvim, Fabienne Micheli, Carlos Priminho Pirovani   +9 more
doaj   +1 more source

A multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers [PDF]

, 2013
BACKGROUND: Chronic Fatigue Syndrome (CFS) is a severe, systemic illness characterized by persistent, debilitating and medically unexplained fatigue. The etiology and pathophysiology of CFS remains obscure, and diagnosis is formulated through the patient’
Antonio Lucacchini, Arianna Consensi, Camillo Giacomelli, Elena Donadio, Fabrizio Maggi, Federica Ciregia, Francesca Sernissi, Laura Bazzichi, Laura Giusti, Pietro Scarpellini, Ylenia Da Valle   +10 more
core   +1 more source

OXidative Stress PREDictor: A Supervised Learning Approach for Annotating Cellular Oxidative Stress States in Inflammatory Cells

Advanced Intelligent Systems, Volume 7, Issue 3, March 2025.
OxSpred, an eXtreme‐Gradient‐Boosting‐‐based supervised learning model, accurately annotates oxidative stress in innate immune cells at the single‐cell level, providing interpretable embeddings with significant biological relevance. This innovative tool revolutionizes the understanding of innate immune cell functions during inflammation and enhances ...
Po‐Yuan Chen, Tai‐Ming Ko
wiley   +1 more source

Tick salivary protein Cystatin: structure, anti-inflammation and molecular mechanism

Ticks and Tick-Borne Diseases
Ticks are blood-sucking ectoparasites that secrete immunomodulatory substances in saliva to hosts during engorging. Cystatins, a tick salivary protein and natural inhibitor of Cathepsins, are attracting growing interest globally because of the ...
Xin Gao, Yuan Tian, Zi-ling Liu, Dan Li, Jia-jun Liu, Guang-xu Yu, De-yong Duan, Tao Peng, Tian-yin Cheng, Lei Liu   +9 more
doaj   +1 more source

Surface Plasmon Resonance Imaging biosensor for cystatin determination based on the application of bromelain, ficin and chymopapain

Folia Histochemica et Cytobiologica, 2012
A Surface Plasmon Resonance Imaging (SPRI) sensor based on bromelain or chymopapain or ficin has been developed for specific cystatin determination.
Anna Sankiewicz, Joanna Breczko, Ewa Gorodkiewicz   +2 more
doaj   +1 more source

Discordance in cathepsin B and cystatin C expressions in bronchoalveolar fluids between murine bleomycin-induced fibrosis and human idiopathic fibrosis [PDF]

, 2015
International audienceAbstractThe activity of cysteine cathepsin B increased markedly in lung homogenates and in bronchoalveolar lavage fluids (BALF) of the mouse model of bleomycin-induced lung fibrosis after 14 days of challenge.
Couillin, Isabelle, Gombault, Aurélie, Kasabova, Mariana, Lalmanach, Gilles, Lecaille, Fabien, Marchand-Adam, Sylvain, Reinheckel, Thomas, Villeret, Bérengère   +7 more
core   +3 more sources