Results 71 to 80 of about 30,295 (249)

An enzymatic assay for high-throughput screening of cytidine-producing microbial strains.

PLoS ONE, 2015
Cytidine is an industrially useful precursor for the production of antiviral compounds and a variety of industrial compounds. Interest in the microbial production of cytidine has grown recently and high-throughput screening of cytidine over-producers is ...
Huina Dong, Yongfei Liu, Xin Zu, Ning Li, Feiran Li, Dawei Zhang   +5 more
doaj   +1 more source

Nature Inspired Delivery Vehicles for CRISPR‐Based Genome Editing

Small, EarlyView.
The review highlights nature‐inspired nanocarriers for CRISPR delivery, emphasizing viral vectors, extracellular vesicles, liposomes, and lipid nanoparticles. It discusses their roles in improving specificity, minimizing immunogenicity, and overcoming barriers in genome editing. Recent advancements, challenges, and therapeutic applications are explored,
Elizabeth Maria Clarissa, Mamata Karmacharya, Hyunmin Choi, Sumit Kumar, Yoon‐Kyoung Cho   +4 more
wiley   +1 more source

Biological function of activation-induced cytidine deaminase (AID)

Biomedical Journal, 2014
Activation-induced Cytidine Deaminase (AID) is an essential regulator of B cell diversification, but its full range of action has until recently been an enigma. Based on homology, it was originally proposed to be an RNA-editing enzyme, but so far, no RNA
Ritu Kumar, Lauren J DiMenna, Jayanta Chaudhuri, Todd Evans   +3 more
doaj   +1 more source

APOBEC-related mutagenesis and neo-peptide hydrophobicity: implications for response to immunotherapy. [PDF]

, 2019
Tumor-associated neo-antigens are mutated peptides that allow the immune system to recognize the affected cell as foreign. Cells carrying excessive mutation load often develop mechanisms of tolerance.
Boichard, Amélie, Frampton, Garrett M, Goodman, Aaron, Kurzrock, Razelle, Lippman, Scott, Pham, Timothy V, Tamayo, Pablo, Tsigelny, Igor F, Yeerna, Huwate   +8 more
core  

Interactions in vivo between the Vif protein of HIV-1 and the precursor (Pr55GAG) of the virion nucleocapsid proteins [PDF]

, 2009
The abnormality of viral core structure seen in vif-defective HIV-1 grown in PBMCs has suggested a role for Vif in viral morphogenesis. Using an in vivo mammalian two-hybrid assay, the interaction between Vif and the precursor (Pr55GAG) of the virion ...
A Harmache, A Mehle, AM Borman, AM Sheehy, AM Sheehy, B Liu, B Liu, B Mangeat, C Tian, DH Gabuzda, DH Gabuzda, Farhatullah Syed, H Ochman, H Zhang, HA Vasavada, I Sadowski, JH Simon, K Luo, K Stopak, L Baraz, L Fan, M Bouyac, M Bouyac, M Boyce, M Marin, MA Adekale, Malcolm A. McCrae, MS Oberste, N Madani, RA Fouchier, RC Desrosiers, RS Harris, S Cen, S Erickson-Viitanen, S Hoglund, SG Conticello, SK Arya, TH Lee, X Yu, Y Gluzman, Y Imai, Y Yu, YH Zheng   +42 more
core   +1 more source

Epigenetic mechanisms and therapeutic innovations in chronic pain‐associated neuropsychiatric co‐morbidities

British Journal of Pharmacology, EarlyView.
Abstract Chronic pain, marked by nociceptive sensitization and maladaptive neuroplasticity, affects 30% of the global population with escalating socioeconomic burdens. Epidemiological data show a 2‐3‐fold increase in neuropsychiatric co‐morbidities among individuals with chronic pain, where epigenetic dysregulation serves as a key mechanism linking ...
Kai Zhang, Sijia Zhu, Na Xing, Zhen Zhou, Jiayi Chen, Tianen Si, Mingrui Li, Lin Jia, Yousef Rastegar‐Kashkooli, Tom J. Wang, Wei Zhu, Jing Li, Chao Jiang, Junmin Wang, Moxin Wu, Xiaoping Yin, Weidong Zang, Jian Wang, Songxue Su   +18 more
wiley   +1 more source

Exploring ABOBEC3A and APOBEC3B substrate specificity and their role in HPV positive head and neck cancer

iScience, 2022
Summary: APOBEC3 family members are cytidine deaminases catalyzing conversion of cytidine to uracil. Many studies have established a link between APOBEC3 expression and cancer development and progression, especially APOBEC3A (A3A) and APOBEC3B (A3B ...
Christina Papini, Zechen Wang, Shalley N. Kudalkar, Travis Parke Schrank, Su Tang, Tomoaki Sasaki, Cory Wu, Brandon Tejada, Samantha J. Ziegler, Yong Xiong, Natalia Issaeva, Wendell G. Yarbrough, Karen S. Anderson   +12 more
doaj   +1 more source

Roles of TIF1β in Leukemic Stem Cell Through SETDB1‐Dependent and Independent Mechanisms

Cancer Science, EarlyView.
In leukemic stem cell, BCR::ABL cooperates with TIF1β to open chromatin at oncogenes and close chromatin at differentiation regulators, driving leukemic reprogramming. In TIF1β‐deficient stem cell, the loss of TIF1β inverts this balance, showing closed chromatin at oncogenes and open chromatin at differentiation regulators.
Mariko Morii, Sho Kubota, Goro Sashida
wiley   +1 more source

Lineage tracing using a Cas9-deaminase barcoding system targeting endogenous L1 elements

Nature Communications, 2019
Lineage tracing has provided new insights into cell fate but defining cellular diversity remains a challenge. Here the authors target endogenous repeat regions in mammalian cells with cytidine deaminase fused to nCas9 to create genetic barcodes for fine ...
Byungjin Hwang, Wookjae Lee, Soo-Young Yum, Yujin Jeon, Namjin Cho, Goo Jang, Duhee Bang   +6 more
doaj   +1 more source

Plant organelle C‐to‐U RNA editing factors can operate successfully in yeast (Saccharomyces cerevisiae) as an easily amenable eukaryotic system for their functional analysis

The FEBS Journal, EarlyView.
Plant‐type pentatricopeptide repeat proteins capable of C‐to‐U RNA editing perform faithfully when expressed in a new heterologous system, the yeast Saccharomyces cerevisiae. They were tested with constitutive and inducible expression and with a set of different solubility tags. PPR56, PPR65, and PPR78 from P.
Shyam Ramanathan, Annika Hanraths, Jingchan Xie, Mareike Schallenberg‐Rüdinger, Volker Knoop   +4 more
wiley   +1 more source