Results 21 to 30 of about 131,177 (265)
Enzymatic approaches for profiling cytosine methylation and hydroxymethylation
Molecular Metabolism, 2022 Background: In mammals, modifications to cytosine bases, particularly in cytosine-guanine (CpG) dinucleotide contexts, play a major role in shaping the epigenome.
Tong Wang +2 more
doaj +1 more source
A mutant of uracil DNA glycosylase that distinguishes between cytosine and 5-methylcytosine. [PDF]
PLoS ONE, 2014 We demonstrate that a mutant of uracil DNA glycosylase (N123D:L191A) distinguishes between cytosine and methylcytosine. Uracil DNA glycosylase (UDG) efficiently removes uracil from DNA in a reaction in which the base is flipped into the enzyme's active ...
Scott T Kimber, Tom Brown, Keith R Fox
doaj +1 more source
Factors Influencing the Nitrogen-Source Dependent Flucytosine Resistance in Cryptococcus Species
mBio, 2023 Flucytosine (5-FC) is an antifungal agent commonly used for treatment of cryptococcosis and several other systemic mycoses. In fungi, cytosine permease and cytosine deaminase are known major players in flucytosine resistance by regulating uptake and ...
Dong-Hoon Yang +3 more
doaj +1 more source
Effectiveness of the enzyme cytosine deaminase in the free living ciliary organism Tetrahymena pyriformis (Ehrenberg, 1830) [PDF]
مجلة التربية والعلم, 2008 Cytosine deaminase (EC.3.5.4.1) activity and properties was determined in the extract of the free living ciliata Tetrahymena pyriformis grown in proteose peptone medium. The optimum conditions for the enzyme activity was also determined and it was found
Zahraa Bashi, Muna Jankeer
doaj +1 more source
FEBS Letters, EarlyView.Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil +4 more
wiley +1 more source
South Asian Journal of Cancer, 2016 Aim: Lung cancer is considered to be the most common cancer in the world. In humans, about 50% or more cancers have a mutated tumor suppressor p53 gene thereby resulting in accumulation of p53 protein and losing its function to activate the target genes ...
Rashid Mir +9 more
doaj +1 more source
eLife, 2021 Shotgun metagenomic sequencing is a powerful approach to study microbiomes in an unbiased manner and of increasing relevance for identifying novel enzymatic functions.
Weiwei Yang +9 more
doaj +1 more source
Molecular Oncology, EarlyView.A urine‐based digital PCR assay targeting two hotspot TERT promoter variants detected bladder cancer with high sensitivity and no false positives in this case–control cohort. The streamlined AbsoluteQ workflow outperformed Sanger sequencing and supports non‐invasive molecular testing for bladder cancer detection.
Anna Nykel +12 more
wiley +1 more source
Frontiers in Genetics, 2015 DNA cytosine methylation is a widespread epigenetic mechanism in eukaryotes, and plant genomes commonly are densely methylated. Genomic methylation can be associated with functional consequences such as mutational events, genomic instability or altered ...
Conchita eAlonso +3 more
doaj +1 more source
Results in Chemistry, 2022 Quartz crystal resonators coated with gas-sorptive films were characterized by analyzing their structures and their organic gas-sorption behaviors, which were then studied using statistics and computational molecular simulations.
Iwao Sugimoto +2 more
doaj +1 more source