Results 11 to 20 of about 3,096 (155)

A deadenylase assay by size-exclusion chromatography. [PDF]

PLoS ONE, 2012
The shortening of the 3'-end poly(A) tail, also called deadenylation, is crucial to the regulation of mRNA processing, transportation, translation and degradation.
Guang-Jun He, Yong-Bin Yan
doaj   +4 more sources

Biochemical and biophysical characterization of the deadenylase CrCaf1 from Chlamydomonas reinhardtii. [PDF]

PLoS ONE, 2013
The modulation of mRNA turnover has been increasingly recognized as a hotpoint for gene expression regulation at the post-transcriptional level. In eukaryotic cells, most mRNAs are degraded via the deadenylation-dependent pathway, in which the removal of
Jia-Quan Zhang, Guang-Jun He, Yong-Bin Yan   +2 more
doaj   +4 more sources

Integrated Deadenylase Genetic Association Network and Transcriptome Analysis in Thoracic Carcinomas

Molecules, 2022
The poly(A) tail at the 3′ end of mRNAs determines their stability, translational efficiency, and fate. The shortening of the poly(A) tail, and its efficient removal, triggers the degradation of mRNAs, thus, regulating gene expression.
Athanasios Kyritsis, Eirini Papanastasi, Ioanna Kokkori, Panagiotis Maragozidis, Demetra S. M. Chatzileontiadou, Paschalina Pallaki, Maria Labrou, Sotirios G. Zarogiannis, George P. Chrousos, Dimitrios Vlachakis, Konstantinos I. Gourgoulianis, Nikolaos A. A. Balatsos   +11 more
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The E3 ubiquitin ligase, RNF219, suppresses CNOT6L expression to exhibit antiproliferative activity. [PDF]

FEBS Open Bio
We identified RNF219 as a CCR4‐NOT complex‐interacting E3 ubiquitin ligase that targets the CCR4‐NOT subunit CNOT6L for ubiquitination. RNF219 directly binds to the DUF3819 domain of CNOT1 through its putative α‐helix spanning amino acids 521–542. Our findings also suggest that antiproliferative activity of RNF219 is at least partially mediated by ...
Soeda S, Montrose M, Takahashi A, Ishida R, Burriel S, Ohmine N, Natsume T, Adachi S, Kim M, Yamamoto T.   +9 more
europepmc   +2 more sources

Translational Repression by Deadenylases [PDF]

Journal of Biological Chemistry, 2010
The CCR4-CAF1-NOT complex is a major cytoplasmic deadenylation complex in yeast and mammals. This complex associates with RNA-binding proteins and microRNAs to repress translation of target mRNAs. We sought to determine how CCR4 and CAF1 participate in repression and control of maternal mRNAs using Xenopus laevis oocytes.
Cooke, Amy, Priggie, Andrew, Wickens, Marvin   +2 more
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BTG/TOB factors impact deadenylases [PDF]

Trends in Biochemical Sciences, 2009
BTG/TOB factors are a family of antiproliferative proteins whose expression is altered in numerous cancers. They have been implicated in cell differentiation, development and apoptosis. Although proposed to affect transcriptional regulation, these factors interact with CAF1, a subunit of the main eukaryotic deadenylase, and with poly(A)-binding ...
Mauxion, Fabienne, Chen, Chyi-Ying A, Séraphin, Bertrand, Shyu, Ann-Bin   +3 more
openaire   +2 more sources

Expanding the repertoire of deadenylases [PDF]

RNA Biology, 2017
Deadenylases belong to an expanding family of exoribonucleases involved mainly in mRNA stability and turnover, with the exception of PARN which has additional roles in the biogenesis of several important non-coding RNAs, including miRNAs and piRNAs. Recently, PARN in C. elegans and its homolog PNLDC1 in B.
Ilias, Skeparnias, Dimitrios, Αnastasakis, Athanasios-Nasir, Shaukat, Katerina, Grafanaki, Constantinos, Stathopoulos   +4 more
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A Comprehensive Phylogenetic Analysis of Deadenylases [PDF]

Evolutionary Bioinformatics, 2013
Deadenylases catalyze the shortening of the poly(A) tail at the messenger ribonucleic acid (mRNA) 3′-end in eukaryotes. Therefore, these enzymes influence mRNA decay, and constitute a major emerging group of promising anti-cancer pharmacological targets.
Vlachakis, Dimitrios, Pavlopoulou, ATHANASIA, Kossida, Sophia, Balatsos, Nikolaos A. A.   +3 more
openaire   +5 more sources

Opposing Polymerase-Deadenylase Activities Regulate Cytoplasmic Polyadenylation [PDF]

Molecular Cell, 2006
Cytoplasmic polyadenylation is one mechanism that regulates translation in early animal development. In Xenopus oocytes, polyadenylation of dormant mRNAs, including cyclin B1, is controlled by the cis-acting cytoplasmic polyadenylation element (CPE) and hexanucleotide AAUAAA through associations with CPEB and CPSF, respectively.
Kim, Jong Heon, Richter, Joel D.
openaire   +2 more sources

Human Ccr4–Not complexes contain variable deadenylase subunits [PDF]

Biochemical Journal, 2009
The Ccr4–Not complex is evolutionarily conserved and important for regulation of mRNA synthesis and decay. The composition of the yeast complex has been well described. Orthologues of the yeast Ccr4–Not components have been identified in human cells including multiple subunits with mRNA deadenylase activity.
Lau, N.C., Kolkman, A., van Schaik, F.M.A., Mulder, K.W., Pijnappel, W.W.M., Heck, A.J.R., Timmers, H.T.M.   +6 more
openaire   +3 more sources