Results 21 to 30 of about 6,008 (204)

Expanding the repertoire of deadenylases [PDF]

RNA Biology, 2017
Deadenylases belong to an expanding family of exoribonucleases involved mainly in mRNA stability and turnover, with the exception of PARN which has additional roles in the biogenesis of several important non-coding RNAs, including miRNAs and piRNAs. Recently, PARN in C. elegans and its homolog PNLDC1 in B.
Ilias, Skeparnias, Dimitrios, Αnastasakis, Athanasios-Nasir, Shaukat, Katerina, Grafanaki, Constantinos, Stathopoulos   +4 more
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A Comprehensive Phylogenetic Analysis of Deadenylases [PDF]

Evolutionary Bioinformatics, 2013
Deadenylases catalyze the shortening of the poly(A) tail at the messenger ribonucleic acid (mRNA) 3′-end in eukaryotes. Therefore, these enzymes influence mRNA decay, and constitute a major emerging group of promising anti-cancer pharmacological targets.
Vlachakis, Dimitrios, Pavlopoulou, ATHANASIA, Kossida, Sophia, Balatsos, Nikolaos A. A.   +3 more
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Opposing Polymerase-Deadenylase Activities Regulate Cytoplasmic Polyadenylation [PDF]

Molecular Cell, 2006
Cytoplasmic polyadenylation is one mechanism that regulates translation in early animal development. In Xenopus oocytes, polyadenylation of dormant mRNAs, including cyclin B1, is controlled by the cis-acting cytoplasmic polyadenylation element (CPE) and hexanucleotide AAUAAA through associations with CPEB and CPSF, respectively.
Kim, Jong Heon, Richter, Joel D.
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Human Ccr4–Not complexes contain variable deadenylase subunits [PDF]

Biochemical Journal, 2009
The Ccr4–Not complex is evolutionarily conserved and important for regulation of mRNA synthesis and decay. The composition of the yeast complex has been well described. Orthologues of the yeast Ccr4–Not components have been identified in human cells including multiple subunits with mRNA deadenylase activity.
Lau, N.C., Kolkman, A., van Schaik, F.M.A., Mulder, K.W., Pijnappel, W.W.M., Heck, A.J.R., Timmers, H.T.M.   +6 more
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Antagonistic control of Caenorhabditis elegans germline stem cell proliferation and differentiation by PUF proteins FBF-1 and FBF-2

eLife, 2020
Stem cells support tissue maintenance, but the mechanisms that coordinate the rate of stem cell self-renewal with differentiation at a population level remain uncharacterized. We find that two PUF family RNA-binding proteins FBF-1 and FBF-2 have opposite
Xiaobo Wang, Mary Ellenbecker, Benjamin Hickey, Nicholas J Day, Emily Osterli, Mikaya Terzo, Ekaterina Voronina   +6 more
doaj   +1 more source

The CCR4–NOT Deadenylase Complex Maintains Adipocyte Identity [PDF]

International Journal of Molecular Sciences, 2019
Shortening of poly(A) tails triggers mRNA degradation; hence, mRNA deadenylation regulates many biological events. In the present study, we generated mice lacking the Cnot1 gene, which encodes an essential scaffold subunit of the CCR4–NOT deadenylase complex in adipose tissues (Cnot1-AKO mice) and we examined the role of CCR4–NOT in adipocyte function.
Akinori Takahashi, Shohei Takaoka, Shungo Kobori, Tomokazu Yamaguchi, Sara Ferwati, Keiji Kuba, Tadashi Yamamoto, Toru Suzuki   +7 more
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Deadenylase depletion protects inherited mRNAs in primordial germ cells [PDF]

Development, 2014
A crucial event in animal development is the specification of primordial germ cells (PGCs), which become the stem cells that create sperm and eggs. How PGCs are created provides a valuable paradigm for understanding stem cells in general. We find that the PGCs of the sea urchin Strongylocentrotus purpuratus exhibit broad transcriptional repression, yet
Swartz, S. Z., Reich, A. M., Oulhen, N., Raz, T., Milos, P. M., Campanale, J. P., Hamdoun, A., Wessel, G. M.   +7 more
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Biochemical and in silico identification of the active site and the catalytic mechanism of the circadian deadenylase HESPERIN

FEBS Open Bio, 2022
The 24‐h molecular clock is based on the stability of rhythmically expressed transcripts. The shortening of the poly(A) tail of mRNAs is often the first and rate‐limiting step that determines the lifespan of a mRNA and is catalyzed by deadenylases ...
Rafailia A. A. Beta, Athanasios Kyritsis, Veroniki Douka, Eirini Papanastasi, Marianna Rizouli, Demetres D. Leonidas, Dimitrios Vlachakis, Nikolaos A. A. Balatsos   +7 more
doaj   +1 more source

The yeast PUF protein Puf5 has Pop2-independent roles in response to DNA replication stress. [PDF]

PLoS ONE, 2010
PUFs are RNA binding proteins that promote mRNA deadenylation and decay and inhibit translation. Yeast Puf5 is the prototype for studying PUF-dependent gene repression.
Ana Traven, Tricia L Lo, Trevor Lithgow, Jörg Heierhorst   +3 more
doaj   +1 more source

Heterogeneity and complexity within the nuclease module of the Ccr4-Not complex

Frontiers in Genetics, 2013
The shortening of the poly(A) tail of cytoplasmic mRNA (deadenylation) is a pivotal step in the regulation of gene expression in eukaryotic cells. Deadenylation impacts on both regulated mRNA decay as well as the rate of mRNA translation.
Gerlof Sebastiaan Winkler, Dario L Balacco   +1 more
doaj   +1 more source