A deadenylase assay by size-exclusion chromatography. [PDF]
PLoS ONE, 2012 The shortening of the 3'-end poly(A) tail, also called deadenylation, is crucial to the regulation of mRNA processing, transportation, translation and degradation.
Guang-Jun He, Yong-Bin Yan
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BTG/TOB factors impact deadenylases. [PDF]
Trends in Biochemical Sciences, 2009 BTG/TOB factors are a family of antiproliferative proteins whose expression is altered in numerous cancers. They have been implicated in cell differentiation, development and apoptosis.
Chen, Chyi-Ying A +3 more
core +4 more sources
Biochemical and biophysical characterization of the deadenylase CrCaf1 from Chlamydomonas reinhardtii. [PDF]
PLoS ONE, 2013 The modulation of mRNA turnover has been increasingly recognized as a hotpoint for gene expression regulation at the post-transcriptional level. In eukaryotic cells, most mRNAs are degraded via the deadenylation-dependent pathway, in which the removal of
Jia-Quan Zhang +2 more
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Integrated Deadenylase Genetic Association Network and Transcriptome Analysis in Thoracic Carcinomas
Molecules, 2022 The poly(A) tail at the 3′ end of mRNAs determines their stability, translational efficiency, and fate. The shortening of the poly(A) tail, and its efficient removal, triggers the degradation of mRNAs, thus, regulating gene expression.
Athanasios Kyritsis +11 more
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Post-transcriptional Control of Tumor Cell Autonomous Metastatic Potential by CCR4-NOT Deadenylase CNOT7. [PDF]
PLoS Genetics, 2016 Accumulating evidence supports the role of an aberrant transcriptome as a driver of metastatic potential. Deadenylation is a general regulatory node for post-transcriptional control by microRNAs and other determinants of RNA stability.
Farhoud Faraji +6 more
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Stoichiometry and Change of the mRNA Closed-Loop Factors as Translating Ribosomes Transit from Initiation to Elongation [PDF]
, 2016 Protein synthesis is a highly efficient process and is under exacting control. Yet, the actual abundance of translation factors present in translating complexes and how these abundances change during the transit of a ribosome across an mRNA remains ...
Chiang, Yueh-Chin +6 more
core +19 more sources
Pan2-Pan3 Complex-Mediated Deadenylation Enforces mRNA Quality Control for Infection of the Rice Blast Fungus. [PDF]
Adv Sci (Weinh)Discovery that deadenylation, rather than transcription, acts as the rate‐limiting step for developmental timing in a plant pathogen. Evidence that P‐body integrity and mRNA decay are mechanistically coupled to rapid cellular differentiation under environmental stress. Identification of Pan2‐Pan3 as a pathogen‐specific “meta‐virulence factor” absent in
Lv Z +6 more
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The E3 ubiquitin ligase, RNF219, suppresses CNOT6L expression to exhibit antiproliferative activity. [PDF]
FEBS Open BioWe identified RNF219 as a CCR4‐NOT complex‐interacting E3 ubiquitin ligase that targets the CCR4‐NOT subunit CNOT6L for ubiquitination. RNF219 directly binds to the DUF3819 domain of CNOT1 through its putative α‐helix spanning amino acids 521–542. Our findings also suggest that antiproliferative activity of RNF219 is at least partially mediated by ...
Soeda S +9 more
europepmc +2 more sources
RNF219 regulates CCR4-NOT function in mRNA translation and deadenylation
Scientific Reports, 2022 Post-transcriptional regulatory mechanisms play a role in many biological contexts through the control of mRNA degradation, translation and localization.
Aude Guénolé +7 more
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Translational Repression by Deadenylases [PDF]
Journal of Biological Chemistry, 2010 The CCR4-CAF1-NOT complex is a major cytoplasmic deadenylation complex in yeast and mammals. This complex associates with RNA-binding proteins and microRNAs to repress translation of target mRNAs. We sought to determine how CCR4 and CAF1 participate in repression and control of maternal mRNAs using Xenopus laevis oocytes.
Cooke, Amy +2 more
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