Results 11 to 20 of about 3,926 (154)

Biochemical and in silico identification of the active site and the catalytic mechanism of the circadian deadenylase HESPERIN

FEBS Open Bio, 2022
The 24‐h molecular clock is based on the stability of rhythmically expressed transcripts. The shortening of the poly(A) tail of mRNAs is often the first and rate‐limiting step that determines the lifespan of a mRNA and is catalyzed by deadenylases ...
Rafailia A. A. Beta, Athanasios Kyritsis, Veroniki Douka, Eirini Papanastasi, Marianna Rizouli, Demetres D. Leonidas, Dimitrios Vlachakis, Nikolaos A. A. Balatsos   +7 more
doaj   +3 more sources

Mechanisms governing poly(A)-tail-length specificity of the human PAN2-PAN3 deadenylase complex

Cell Reports
Summary: The lifespan of most eukaryotic mRNAs is modulated by the gradual shortening of the poly(A) tail and removal of the associated poly(A)-binding protein.
Jana C. Albrecht, Timo Reitinger, Jérôme Basquin, Steffen Schüssler, Margot Riggi, Ingmar B. Schäfer, Elena Conti   +6 more
doaj   +2 more sources

The anti-proliferative activity of BTG/TOB proteins is mediated via the Caf1a (CNOT7) and Caf1b (CNOT8) deadenylase subunits of the Ccr4-not complex.

PLoS ONE, 2012
The human BTG/TOB protein family comprises six members (BTG1, BTG2/PC3/Tis21, BTG3/Ana, BTG4/PC3B, TOB1/Tob, and TOB2) that are characterised by a conserved BTG domain.
Rachel Doidge, Saloni Mittal, Akhmed Aslam, G Sebastiaan Winkler   +3 more
doaj   +2 more sources

Integrated Deadenylase Genetic Association Network and Transcriptome Analysis in Thoracic Carcinomas

Molecules, 2022
The poly(A) tail at the 3′ end of mRNAs determines their stability, translational efficiency, and fate. The shortening of the poly(A) tail, and its efficient removal, triggers the degradation of mRNAs, thus, regulating gene expression.
Athanasios Kyritsis, Eirini Papanastasi, Ioanna Kokkori, Panagiotis Maragozidis, Demetra S. M. Chatzileontiadou, Paschalina Pallaki, Maria Labrou, Sotirios G. Zarogiannis, George P. Chrousos, Dimitrios Vlachakis, Konstantinos I. Gourgoulianis, Nikolaos A. A. Balatsos   +11 more
doaj   +3 more sources

Biochemical and biophysical characterization of the deadenylase CrCaf1 from Chlamydomonas reinhardtii. [PDF]

PLoS ONE, 2013
The modulation of mRNA turnover has been increasingly recognized as a hotpoint for gene expression regulation at the post-transcriptional level. In eukaryotic cells, most mRNAs are degraded via the deadenylation-dependent pathway, in which the removal of
Jia-Quan Zhang, Guang-Jun He, Yong-Bin Yan   +2 more
doaj   +2 more sources

Genome-Wide Mapping of Decay Factor–mRNA Interactions in Yeast Identifies Nutrient-Responsive Transcripts as Targets of the Deadenylase Ccr4

G3: Genes, Genomes, Genetics, 2018
The Ccr4 (carbon catabolite repression 4)-Not complex is a major regulator of stress responses that controls gene expression at multiple levels, from transcription to mRNA decay.
Jason E. Miller, Liye Zhang, Haoyang Jiang, Yunfei Li, B. Franklin Pugh, Joseph C. Reese   +5 more
doaj   +3 more sources

The PARN deadenylase targets a discrete set of mRNAs for decay and regulates cell motility in mouse myoblasts.

PLoS Genetics, 2012
PARN is one of several deadenylase enzymes present in mammalian cells, and as such the contribution it makes to the regulation of gene expression is unclear.
Jerome E Lee, Ju Youn Lee, Jarrett Trembly, Jeffrey Wilusz, Bin Tian, Carol J Wilusz   +5 more
doaj   +2 more sources

ER-Localized Deadenylase <i>PNLDC1</i> Suppresses Colorectal Cancer Progression by Targeting the mRNA Decay of <i>TUBB4B</i>. [PDF]

Exploration (Beijing)
The deficiency of PNLDC1 exacerbates the advancement of CRC. PNLDC1 interacts with TUBB4B mRNA to regulate the p53–p21–CDK2–cyclin E2 pathway, thereby influencing the G2/M phase of the cell cycle. ABSTRACT Colorectal cancer (CRC) remains a leading cause of cancer‐related mortality, highlighting the urgent need for novel therapeutic strategies.
Liu L, Guan Y, Qin Y, Chen G, Wang G, Yan X, Li X, Sun L, Yu W, Ouyang S, Ma Z, Xu Z, Cao Z, Yu F, Zhou T, Luo Z, Chen X, Yan Y, Hu L.   +18 more
europepmc   +2 more sources

Molecular Interplay of PARN and Telomerase: Tail Modifiers and Disease Implications. [PDF]

Wiley Interdiscip Rev RNA
Schematic representation of the molecular interplay between PARN, telomerase‐associated, and the involvement of p53 in regulating telomere maintenance and genome stability. The top panel shows how normal PARN levels are involved in regulating p53 levels and balanced telomerase activity through their regulatory interaction with TERC and TERRA, thus ...
Felicitus S, Pinto SS, Madagundapalli Ramakrishna SS, Nanjappa DP, Chakraborty G, Chakraborty A.   +5 more
europepmc   +2 more sources

Antagonistic control of Caenorhabditis elegans germline stem cell proliferation and differentiation by PUF proteins FBF-1 and FBF-2

eLife, 2020
Stem cells support tissue maintenance, but the mechanisms that coordinate the rate of stem cell self-renewal with differentiation at a population level remain uncharacterized. We find that two PUF family RNA-binding proteins FBF-1 and FBF-2 have opposite
Xiaobo Wang, Mary Ellenbecker, Benjamin Hickey, Nicholas J Day, Emily Osterli, Mikaya Terzo, Ekaterina Voronina   +6 more
doaj   +1 more source