Results 41 to 50 of about 3,192 (173)

Deadenylase depletion protects inherited mRNAs in primordial germ cells [PDF]

Development, 2014
A crucial event in animal development is the specification of primordial germ cells (PGCs), which become the stem cells that create sperm and eggs. How PGCs are created provides a valuable paradigm for understanding stem cells in general. We find that the PGCs of the sea urchin Strongylocentrotus purpuratus exhibit broad transcriptional repression, yet
Swartz, S. Z., Reich, A. M., Oulhen, N., Raz, T., Milos, P. M., Campanale, J. P., Hamdoun, A., Wessel, G. M.   +7 more
openaire   +2 more sources

Discovery of Substituted 5-(2-Hydroxybenzoyl)-2-Pyridone Analogues as Inhibitors of the Human Caf1/CNOT7 Ribonuclease

Molecules
The Caf1/CNOT7 nuclease is a catalytic component of the Ccr4-Not deadenylase complex, which is a key regulator of post-transcriptional gene regulation. In addition to providing catalytic activity, Caf1/CNOT7 and its paralogue Caf1/CNOT8 also contribute a
Ishwinder Kaur, Gopal P. Jadhav, Peter M. Fischer, Gerlof Sebastiaan Winkler   +3 more
doaj   +1 more source

In Vitro Analysis of RNA Degradation Catalyzed by Deadenylase Enzymes [PDF]

, 2014
In this chapter, we describe a method for purification and analysis of the enzymatic activity of deadenylase enzymes. Nearly all eukaryotic messenger RNAs are modified at the 3' end by the addition of an adenosine polymer: the poly-adenosine tail. The poly(A) tail plays a central role in protein expression and mRNA fate.
Joel, Hrit, Nathan, Raynard, Jamie, Van Etten, Kamya, Sankar, Adam, Petterson, Aaron C, Goldstrohm   +5 more
openaire   +2 more sources

Breaking the A chain: regulating mRNAs in development through CCR4 deadenylase [PDF]

F1000 Biology Reports, 2009
Post-transcriptional mechanisms of gene regulation have long been implicated in specifying embryonic pattern in many organisms. Experiments in Caenorhabditis elegans, Drosophila, and Xenopus have recently converged, pointing to the CCR4 deadenylase complex as a key effector that modulates the expression of proteins from specific germline mRNAs.
Moore, Jocelyn, Lasko, Paul
openaire   +2 more sources

The eIF4E-Binding Protein 4E-T Is a Component of the mRNA Decay Machinery that Bridges the 5′ and 3′ Termini of Target mRNAs

Cell Reports, 2015
Eukaryotic mRNA degradation often initiates with the recruitment of the CCR4-NOT deadenylase complex and decay factors to the mRNA 3′ terminus. How the 3′-proximal decay machinery interacts with the 5′-terminal cap structure in order to engender mRNA ...
Tamiko Nishimura, Zoya Padamsi, Hana Fakim, Simon Milette, Wade H. Dunham, Anne-Claude Gingras, Marc R. Fabian   +6 more
doaj   +1 more source

Time-resolved single-cell sequencing identifies multiple waves of mRNA decay during the mitosis-to-G1 phase transition

eLife, 2022
Accurate control of the cell cycle is critical for development and tissue homeostasis, and requires precisely timed expression of many genes. Cell cycle gene expression is regulated through transcriptional and translational control, as well as through ...
Lenno Krenning, Stijn Sonneveld, Marvin E Tanenbaum   +2 more
doaj   +1 more source

A mutation in cnot8, component of the Ccr4-not complex regulating transcript stability, affects expression levels of developmental regulators and reveals a role of Fgf3 in development of caudal hypothalamic dopaminergic neurons. [PDF]

PLoS ONE, 2014
While regulation of the activity of developmental control genes at the transcriptional level as well as by specific miRNA-based degradation are intensively studied, little is known whether general cellular mechanisms controlling mRNA decay may contribute
Peter Koch, Heiko B Löhr, Wolfgang Driever   +2 more
doaj   +1 more source

Investigating the function of CAF1 deadenylases during plant stress responses [PDF]

Plant Signaling & Behavior, 2010
Alteration of gene expression plays a central role in the transmission of developmental and environmental signals. The steady-state transcript level within a cell is determined by the combination of the rate synthesis and the rate of degradation. While altering the rate of mRNA turnover is known to provide a rapid mechanism to reprogram transcript ...
Justin W, Walley, Dior R, Kelley, Tatyan, Savchenko, Katayoon, Dehesh   +3 more
openaire   +2 more sources

New roles for nuclear EGFR in regulating the stability and translation of mRNAs associated with VEGF signaling. [PDF]

PLoS ONE, 2017
Cell membrane-associated epidermal growth factor receptor (EGFR) translocates into a perinuclear/nuclear location upon stimulation, where it complexes with mRNAs.
Klaus Dittmann, Claus Mayer, Stefan Czemmel, Stephan M Huber, H Peter Rodemann   +4 more
doaj   +1 more source

The RNA‐Binding Protein PARN Remodeled 3′ UTR Structure Defines Poly(A)‐Loading Sites to Mediate Immunoglobulin Homeostasis

Advanced Science, EarlyView.
ABSTRACT Class Switch Recombination (CSR) is essential for generating high‐affinity antibody isotypes from IgM during adaptive humoral responses. Despite well‐established roles for various transcription factors, whether CSR is subject to dedicated post‐transcriptional control represents a significant gap in knowledge.
Siyuan Sun, Chen Yang, Xiaoyu Wang, Naijing Hu, Shengyao Zhang, Xinchun Li, Yu Chen, Jianan Zhai, Xiangfeng Tang, Wei Lu, Chenxu Lu, Changchang Cao, Weiru Yu, Wenhao Wu, Xiaonan Nie, Fengchao Wang, Bing Fang, Fazheng Ren, Yixuan Li, Juan Chen   +19 more
wiley   +1 more source