PTRE-seq reveals mechanism and interactions of RNA binding proteins and miRNAs [PDF]
, 2018 A large number of RNA binding proteins (RBPs) and miRNAs bind to the 3′ untranslated regions of mRNA, but methods to dissect their function and interactions are lacking.
Chaudhari, Hemangi G +3 more
core +3 more sources
eLife, 2022 Accurate control of the cell cycle is critical for development and tissue homeostasis, and requires precisely timed expression of many genes. Cell cycle gene expression is regulated through transcriptional and translational control, as well as through ...
Lenno Krenning +2 more
doaj +1 more source
curledEncodes the Drosophila Homolog of the Vertebrate Circadian Deadenylase Nocturnin [PDF]
Genetics, 2009 AbstractDrosophila melanogaster curled, one of the first fly mutants described by T. H. Morgan >90 years ago, is the founding member of a series of curled wing phenotype mutants widely used as markers in fruit fly genetics. The expressivity of the wing phenotype is environmentally modulated, suggesting that the mutation affects the metabolic ...
Grönke, S. +4 more
openaire +3 more sources
A mutation in cnot8, component of the Ccr4-not complex regulating transcript stability, affects expression levels of developmental regulators and reveals a role of Fgf3 in development of caudal hypothalamic dopaminergic neurons. [PDF]
PLoS ONE, 2014 While regulation of the activity of developmental control genes at the transcriptional level as well as by specific miRNA-based degradation are intensively studied, little is known whether general cellular mechanisms controlling mRNA decay may contribute
Peter Koch +2 more
doaj +1 more source
The decapping activator Edc3 and the Q/N-rich domain of Lsm4 function together to enhance mRNA stability and alter mRNA decay pathway dependence in Saccharomyces cerevisiae [PDF]
, 2016 The rate and regulation of mRNA decay are major elements in the proper control of gene expression. Edc3 and Lsm4 are two decapping activator proteins that have previously been shown to function in the assembly of RNA granules termed P bodies.
Balagopal, Vidya +5 more
core +2 more sources
New roles for nuclear EGFR in regulating the stability and translation of mRNAs associated with VEGF signaling. [PDF]
PLoS ONE, 2017 Cell membrane-associated epidermal growth factor receptor (EGFR) translocates into a perinuclear/nuclear location upon stimulation, where it complexes with mRNAs.
Klaus Dittmann +4 more
doaj +1 more source
Animal Models and Experimental Medicine, EarlyView.This graphical abstract illustrates the essential role of USB1 in neutrophil maturation. In normal myeloid cells, USB1 acts as an RNA exonuclease, trimming RNA tails to maintain proper RNA network function, which supports the differentiation of myeloid progenitor cells into mature neutrophils.
Hang Li +6 more
wiley +1 more source
An Analysis of Putative Roles for the CCR4-NOT Deadenylase-Complex Subunit Regena (NOT2) in microRNA-Mediated Gene Silencing in \u3cem\u3eDrosophila Melanogaster\u3c/em\u3e [PDF]
, 2013 microRNAs (miRNAs) are one class of small non-coding ribonucleic acid (RNA) molecules essential to development and homeostasis in plants and animals. miRNAs silence gene expression through complementary base pairing with target gene messenger RNAs and ...
Erickson, Jasper +3 more
core +1 more source
Molecular medicine of microRNAs: structure, function and implications for diabetes [PDF]
, 2008 MicroRNAs (miRNAs) are a family of endogenous small noncoding RNA molecules, of 19–28 nucleotides in length. In humans, up to 3% of all genes are estimated to encode these evolutionarily conserved sequences.
Duncan +5 more
core +1 more source
In Vitro Analysis of RNA Degradation Catalyzed by Deadenylase Enzymes [PDF]
, 2014 In this chapter, we describe a method for purification and analysis of the enzymatic activity of deadenylase enzymes. Nearly all eukaryotic messenger RNAs are modified at the 3' end by the addition of an adenosine polymer: the poly-adenosine tail. The poly(A) tail plays a central role in protein expression and mRNA fate.
Joel, Hrit +5 more
openaire +2 more sources