Results 21 to 30 of about 3,096 (155)
eLife, 2020 Stem cells support tissue maintenance, but the mechanisms that coordinate the rate of stem cell self-renewal with differentiation at a population level remain uncharacterized. We find that two PUF family RNA-binding proteins FBF-1 and FBF-2 have opposite
Xiaobo Wang +6 more
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The CCR4–NOT Deadenylase Complex Maintains Adipocyte Identity [PDF]
International Journal of Molecular Sciences, 2019 Shortening of poly(A) tails triggers mRNA degradation; hence, mRNA deadenylation regulates many biological events. In the present study, we generated mice lacking the Cnot1 gene, which encodes an essential scaffold subunit of the CCR4–NOT deadenylase complex in adipose tissues (Cnot1-AKO mice) and we examined the role of CCR4–NOT in adipocyte function.
Akinori Takahashi +7 more
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USB1 is a miRNA deadenylase that regulates hematopoietic development
Science, 2023 Mutations in the 3′ to 5′ RNA exonuclease USB1 cause hematopoietic failure in poikiloderma with neutropenia (PN). Although USB1 is known to regulate U6 small nuclear RNA maturation, the molecular mechanism underlying PN remains undetermined, as pre-mRNA splicing is unaffected in patients.
Ho-Chang Jeong +5 more
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FEBS Open Bio, 2022 The 24‐h molecular clock is based on the stability of rhythmically expressed transcripts. The shortening of the poly(A) tail of mRNAs is often the first and rate‐limiting step that determines the lifespan of a mRNA and is catalyzed by deadenylases ...
Rafailia A. A. Beta +7 more
doaj +1 more source
The yeast PUF protein Puf5 has Pop2-independent roles in response to DNA replication stress. [PDF]
PLoS ONE, 2010 PUFs are RNA binding proteins that promote mRNA deadenylation and decay and inhibit translation. Yeast Puf5 is the prototype for studying PUF-dependent gene repression.
Ana Traven +3 more
doaj +1 more source
Deadenylase depletion protects inherited mRNAs in primordial germ cells [PDF]
Development, 2014 A crucial event in animal development is the specification of primordial germ cells (PGCs), which become the stem cells that create sperm and eggs. How PGCs are created provides a valuable paradigm for understanding stem cells in general. We find that the PGCs of the sea urchin Strongylocentrotus purpuratus exhibit broad transcriptional repression, yet
Swartz, S. Z. +7 more
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Heterogeneity and complexity within the nuclease module of the Ccr4-Not complex
Frontiers in Genetics, 2013 The shortening of the poly(A) tail of cytoplasmic mRNA (deadenylation) is a pivotal step in the regulation of gene expression in eukaryotic cells. Deadenylation impacts on both regulated mRNA decay as well as the rate of mRNA translation.
Gerlof Sebastiaan Winkler +1 more
doaj +1 more source
Journal of Inflammation, 2021 Background Tristetraprolin (TTP) family proteins contain conserved tandem CCCH zinc-finger binding to AU-rich elements and C-terminal NOT1-binding domain.
Hsin-Hui Hsieh +8 more
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Human CCR4 deadenylase homolog Angel1 is a non-stop mRNA decay factor. [PDF]
RNA, 2022 AbstractTranslation elongation stalls trigger mRNA decay and degradation of the nascent polypeptide via translation-dependent quality control pathways. One such pathway, non-stop mRNA decay (NSD), targets aberrant mRNAs that lack stop codons for example due to premature polyadenylation.
Nicholson-Shaw T +7 more
europepmc +2 more sources
Investigating the function of CAF1 deadenylases during plant stress responses [PDF]
Plant Signaling & Behavior, 2010 Alteration of gene expression plays a central role in the transmission of developmental and environmental signals. The steady-state transcript level within a cell is determined by the combination of the rate synthesis and the rate of degradation. While altering the rate of mRNA turnover is known to provide a rapid mechanism to reprogram transcript ...
Justin W, Walley +3 more
openaire +2 more sources