Results 121 to 130 of about 14,150 (193)

The role of the E3 ligase Not4 in cotranslational quality control

Frontiers in Genetics, 2014
Cotranslational quality control (QC) is the mechanism by which the cell checks the integrity of newly synthesized proteins and mRNAs. In the event of mistakes these molecules are degraded.
Olesya O Panasenko
doaj   +1 more source

Structural and biochemical characterization of eukaryotic mRNA decapping activators [PDF]

, 2014
In eukaryotes, mRNA turnover starts with the truncation of 3′ poly(A) tail and proceeds with either 3′-to-5′ degradation by the exosome complex or with decapping followed by 5′-to-3′ degradation by Xrn1.
Sharif, Humayun
core  

An mRNA decapping mutant deficient in P body assembly limits mRNA stabilization in response to osmotic stress [PDF]

, 2017
Yeast is exposed to changing environmental conditions and must adapt its genetic program to provide a homeostatic intracellular environment. An important stress for yeast in the wild is high osmolarity.
A Totaro, A van Hoof, AD Aragon, AP Gasch, AR Albig, C Janke, C Miller, C Molin, CJ Decker, CJ Decker, CW Bourque, D Cao, D Melamed, D Teixeira, D Teixeira, D Zheng, E Garre, F Posas, G Gowrishankar, G Haas, G Haimovich, GR Pilkington, H Donis-Keller, J Coller, J Schindelin, J Warringer, J Yale, JA Arribere, JF Garcia, JF Garcia, JR Buchan, JR Buchan, JS Anderson, JS Bett, L Hatfield, L Romero-Santacreu, M Brengues, M Kshirsagar, M Rep, M Tucker, MAM Reijns, N Alhusaini, N Cougot, N Otsu, R Parker, S Hohmann, S Huch, S Jain, S Tharun, T Grousl, T Nissan, U Sheth, U Sheth, V Balagopal, V Hilgers, V Pelechano, V Pelechano, W Hu, Y Uesono   +58 more
core   +1 more source

Stapled Peptides as Inhibitors of mRNA Deadenylation

Angewandte Chemie International Edition
AbstractTherapeutic intervention targeting mRNA typically aims at reducing the levels of disease‐causing sequences. Achieving the opposite effect of blocking the destruction of beneficial mRNA remains underexplored. The degradation of mRNA starts with the removal of poly(A) tails, reducing their stability and translational activity, which is mainly ...
Sunit Pal, Ilja Gordijenko, Stefan Schmeing, Somarghya Biswas, Yasemin Akbulut, Raphael Gasper, Peter 't Hart   +6 more
openaire   +3 more sources

The role of RNA Polymerase II-dependent transcription elongation in the cross-talk between mRNA synthesis and decay. [PDF]

, 2018
The main molecule in gene expression is messenger RNA (mRNA) which transfers the information contained in genes in the nucleus to the cytoplasm where it is translated into proteins that carry out cellular functions. mRNA levels are determined through its
Begley, Victoria Sarah
core  

Deadenylation and turnover of interferon-beta mRNA.

Journal of Biological Chemistry, 1991
The pathway of degradation of human interferon-beta (IFN-beta) mRNA was examined in murine C127 cells that carry an expression vector for this mRNA. The IFN-beta mRNA decayed with a half-life of approximately 45 min in actinomycin D-treated cells and became gradually shorter. This mRNA was superinduced in cycloheximide-treated cells, but it also became
K, Peppel, C, Baglioni
openaire   +2 more sources

Nucleolin‐enhanced deadenylation and bulk translation (566.1)

The FASEB Journal, 2014
Rate of translation is one of the critical factors, determining protein abundance in a cell. After a pioneer round of translation in which 80S ribosome scans the linear mRNA, a cap‐dependent mRNP closed‐loop is formed for bulk translation. Nucleolin interacts with the PABPC and works together for deadenylation and
Ho Hyung Woo, Sang Lee, Setsuko Chambers
openaire   +1 more source

The E3 ubiquitin ligase, RNF219, suppresses CNOT6L expression to exhibit antiproliferative activity

FEBS Open Bio
Despite the increasing evidence of the role of CCR4‐NOT complex in posttranscriptional gene regulation, relatively little is known about its mode of action. In a search for novel CCR4‐NOT interacting partners, we carried out mass spectrometry analysis of
Shou Soeda, Melissa Montrose, Akinori Takahashi, Risa Ishida, Sandrine Burriel, Nao Ohmine, Tohru Natsume, Shungo Adachi, Minsoo Kim, Tadashi Yamamoto   +9 more
doaj   +1 more source

Reconstitution of recombinant human CCR4-NOT reveals molecular insights into regulated deadenylation

Nature Communications, 2019
The CCR4-NOT complex shortens poly(A) tails of messenger RNAs. By biochemical reconstitution of the entire human CCR4-NOT complex, the authors show the stimulatory roles of non-enzymatic subunits and the importance of the interaction between CAF40 and ...
Tobias Raisch, Chung-Te Chang, Yevgen Levdansky, Sowndarya Muthukumar, Stefan Raunser, Eugene Valkov   +5 more
doaj   +1 more source

Most \u3cem\u3eCaenorhabditis elegans\u3c/em\u3e MicroRNAs are Individually Not Essential for Development or Viability [PDF]

, 2007
MicroRNAs (miRNAs), a large class of short noncoding RNAs found in many plants and animals, often act to post-transcriptionally inhibit gene expression. We report the generation of deletion mutations in 87 miRNA genes in Caenorhabditis elegans, expanding
Abbott, Allison L., Alvarez-Saavedra, Ezequiel, Ambros, Victor R., Bartel, David P., Hellman, Andrew B., Horvitz, H. Robert, Lau, Nelson C., McGonagle, Shannon M., Miska, Eric A.   +8 more
core   +1 more source