Results 101 to 110 of about 9,376 (189)

Reconstitution of recombinant human CCR4-NOT reveals molecular insights into regulated deadenylation

, 2019
CCR4-NOT is a conserved multiprotein complex which regulates eukaryotic gene expression principally via shortening of poly(A) tails of messenger RNA or deadenylation. Here, we reconstitute a complete, recombinant human CCR4-NOT complex.
Chang, C., Levdansky, Y., Raisch, T., Muthukumar, S., Raunser, S., Valkov, E.   +5 more
core   +1 more source

The role of the E3 ligase Not4 in cotranslational quality control

Frontiers in Genetics, 2014
Cotranslational quality control (QC) is the mechanism by which the cell checks the integrity of newly synthesized proteins and mRNAs. In the event of mistakes these molecules are degraded.
Olesya O Panasenko
doaj   +1 more source

Deadenylation and turnover of interferon-beta mRNA.

Journal of Biological Chemistry, 1991
The pathway of degradation of human interferon-beta (IFN-beta) mRNA was examined in murine C127 cells that carry an expression vector for this mRNA. The IFN-beta mRNA decayed with a half-life of approximately 45 min in actinomycin D-treated cells and became gradually shorter. This mRNA was superinduced in cycloheximide-treated cells, but it also became
K, Peppel, C, Baglioni
openaire   +2 more sources

The RRM domain of poly(A)-specific ribonuclease has a noncanonical binding site for mRNA cap analog recognition [PDF]

, 2008
The degradation of the poly(A) tail is crucial for posttranscriptional gene regulation and for quality control of mRNA. Poly(A)-specific ribonuclease (PARN) is one of the major mammalian 3’ specific exo-ribonucleases involved in the degradation of the ...
Naohiro Kobayashi, Shirouzu, Mikako, Sakura Suzuki, Nagata, Takashi, Yoshihide Hayashizaki, Takashi Nagata, Yutaka Muto, Takanori Kigawa, Kobayashi, Naohiro, Peter Güntert, Mikako Shirouzu, Muto, Yutaka, Akiko Tanaka, Endo, Ryuta, Makoto Inoue, Hayashizaki, Yoshihide, Terada, Takaho, Inoue, Makoto, Kigawa, Takanori, Suzuki, Sakura, Yokoyama, Shigeyuki, Takaho Terada, Ryuta Endo, Shigeyuki Yokoyama, Güntert, Peter, Tanaka, Akiko   +25 more
core   +2 more sources

PUF3 Acceleration of Deadenylation in Vivo Can Operate Independently of CCR4 Activity, Possibly Involving Effects on the PAB1-mRNP Structure

, 2010
The evolutionarily conserved PUF proteins stimulate CCR4 mRNA deadenylation through binding to 3\u27 untranslated region sequences of specific mRNA. We have investigated the mechanisms by which PUF3 in Saccharomyces cerevisiae accelerates deadenylation ...
Chiang, Yueh-Chin, Yao, Gang, Quigley, Gloria, Lee, Darren, Ohn, Takbum, Denis, Clyde L., Liu, Yuting   +6 more
core   +1 more source

The E3 ubiquitin ligase, RNF219, suppresses CNOT6L expression to exhibit antiproliferative activity

FEBS Open Bio
Despite the increasing evidence of the role of CCR4‐NOT complex in posttranscriptional gene regulation, relatively little is known about its mode of action. In a search for novel CCR4‐NOT interacting partners, we carried out mass spectrometry analysis of
Shou Soeda, Melissa Montrose, Akinori Takahashi, Risa Ishida, Sandrine Burriel, Nao Ohmine, Tohru Natsume, Shungo Adachi, Minsoo Kim, Tadashi Yamamoto   +9 more
doaj   +1 more source

GW182 proteins cause PABP dissociation from silenced miRNA targets in the absence of deadenylation

, 2013
GW182 family proteins interact with Argonaute proteins and are required for the translational repression, deadenylation and decay of miRNA targets. To elicit these effects, GW182 proteins interact with poly(A)-binding protein (PABP) and the CCR4-NOT ...
Izaurralde, E., Kuzuoğlu-Öztürk, D., Zekri, L.   +2 more
core   +1 more source

Substrate-specific regulation of RNA deadenylation in Xenopus embryo and activated egg extracts.

, 1995
International audienceThe poly(A) tail of mRNAs plays an important role in translational control. In Xenopus laevis matured oocytes, maternal mRNAs that contain a cytoplasmic polyadenylation element (CPE) are polyadenylated, whereas CPE deficient mRNAs ...
Legagneux, Vincent, Osborne, Howard Beverley, Omilli, Francis   +2 more
core  

Regulation of EDEN-dependent deadenylation of Aurora A/Eg2-derived mRNA via phosphorylation and dephosphorylation in Xenopus laevis egg extracts.

, 2003
International audienceDeadenylation is an intimate part of the post-transcriptional regulation of maternal mRNAs in embryos. EDEN-BP is so far the only known member of a complex regulating the deadenylation of maternal mRNA in Xenopus laevis embryos in a
Detivaud, Lenaick, Pascreau, Gaetan, Gaëtan Pascreau, Howard B. Osborne, Anthi Karaïskou, Osborne, Howard Beverley, Jacek Z. Kubiak, Kubiak, Jacek, Karaiskou, Anthi, Lénaïck Detivaud   +9 more
core   +1 more source

ATP is dispensable for both miRNA- and Smaug-mediated deadenylation reactions

, 2017
MicroRNAs (miRNAs), as well as the RNA-binding protein Smaug, recruit the CCR4-NOT deadenylase complex for shortening of the poly(A) tail. It has been believed that ATP is required for deadenylation induced by miRNAs or Smaug, based on the fact that the ...
Yukihide Tomari, Sho Niinuma
core   +1 more source