Results 121 to 130 of about 14,275 (206)

Analysis of mRNA deadenylation by multi-protein complexes

Methods, 2017
Poly(A) tails are found at the 3' end of almost every eukaryotic mRNA and are important for the stability of mRNAs and their translation into proteins. Thus, removal of the poly(A) tail, a process called deadenylation, is critical for regulation of gene expression. Most deadenylation enzymes are components of large multi-protein complexes.
Webster, Michael W., Stowell, James A.W., Tang, Terence T.L., Passmore, Lori A.   +3 more
openaire   +3 more sources

Pumilio directs deadenylation-associated translational repression of the cyclin-dependent kinase 1 activator RGC-32 [PDF]

, 2018
Response gene to complement-32 (RGC-32) activates cyclin-dependent kinase 1, regulates the cell cycle and is deregulated in many human tumours. We previously showed that RGC-32 expression is upregulated by the cancer-associated Epstein-Barr virus (EBV ...
Amoroso, Badea, Badea, Baichwal, Bark-Jones, Ben-Bassat, Bernasconi, Blackinton, Bornkamm, Borson, Bredel, Brown, C David Wood, Cameron, Campbell, Cao, Claire Shannon-Lowe, Cordier, Cui, Cui, Donninger, Edwards, Fosbrink, Fosbrink, Friend, Galgano, Gerber, Goldstrohm, Goldstrohm, Graber, Gregory, Gregory, Guo, Hann, Incarnato, Janicke, Kang, Kedde, Kotani, Krauer, Kronja, Lu, Mauser, McClellan, Michelle J West, Michèle Brocard, Miles, Murata, Nakahata, Nikitin, Olivas, O’Nions, Padmanabhan, Pique, Quenault, Rooney, Saha, Saigusa, Sarika Khasnis, Schlick, Sigl, Stumpf, Takada, Tang, Tegla, Van Etten, Venkataraman, Vessey, von Roretz, Weidmann, Weidmann, West, White, Wood   +73 more
core   +1 more source

External and internal triggers of cell death in yeast [PDF]

, 2016
In recent years, yeast was confirmed as a useful eukaryotic model system to decipher the complex mechanisms and networks occurring in higher eukaryotes, particularly in mammalian cells, in physiological as well in pathological conditions.
FALCONE, Claudio, MAZZONI, Cristina
core   +2 more sources

Stapled Peptides as Inhibitors of mRNA Deadenylation

Angewandte Chemie International Edition
AbstractTherapeutic intervention targeting mRNA typically aims at reducing the levels of disease‐causing sequences. Achieving the opposite effect of blocking the destruction of beneficial mRNA remains underexplored. The degradation of mRNA starts with the removal of poly(A) tails, reducing their stability and translational activity, which is mainly ...
Sunit Pal, Ilja Gordijenko, Stefan Schmeing, Somarghya Biswas, Yasemin Akbulut, Raphael Gasper, Peter 't Hart   +6 more
openaire   +3 more sources

The role of the E3 ligase Not4 in cotranslational quality control

Frontiers in Genetics, 2014
Cotranslational quality control (QC) is the mechanism by which the cell checks the integrity of newly synthesized proteins and mRNAs. In the event of mistakes these molecules are degraded.
Olesya O Panasenko
doaj   +1 more source

Translation efficiency is a determinant of the magnitude of miRNA-mediated repression [PDF]

, 2017
MicroRNAs are well known regulators of mRNA stability and translation. However, the magnitude of both translational repression and mRNA decay induced by miRNA binding varies greatly between miRNA targets.
Cottrell, Kyle A, Djuranovic, Sergej, Szczesny, Pawel   +2 more
core   +3 more sources

HuR protein attenuates miRNA-mediated repression by promoting miRISC dissociation from the target RNA [PDF]

, 2017
The microRNA (miRNA)-mediated repression of protein synthesis in mammalian cells is a reversible process. Target mRNAs with regulatory AU-rich elements (AREs) in their 3′-untranslated regions (3′-UTR) can be relieved of miRNA repression under cellular ...
Bhattacharyya, Suvendra N., Fabian, Marc R., Filipowicz, Witold, Kundu, Pradipta, Sonenberg, Nahum   +4 more
core  

Deadenylation and turnover of interferon-beta mRNA.

Journal of Biological Chemistry, 1991
The pathway of degradation of human interferon-beta (IFN-beta) mRNA was examined in murine C127 cells that carry an expression vector for this mRNA. The IFN-beta mRNA decayed with a half-life of approximately 45 min in actinomycin D-treated cells and became gradually shorter. This mRNA was superinduced in cycloheximide-treated cells, but it also became
K, Peppel, C, Baglioni
openaire   +2 more sources

Nucleolin‐enhanced deadenylation and bulk translation (566.1)

The FASEB Journal, 2014
Rate of translation is one of the critical factors, determining protein abundance in a cell. After a pioneer round of translation in which 80S ribosome scans the linear mRNA, a cap‐dependent mRNP closed‐loop is formed for bulk translation. Nucleolin interacts with the PABPC and works together for deadenylation and
Ho Hyung Woo, Sang Lee, Setsuko Chambers
openaire   +1 more source

The E3 ubiquitin ligase, RNF219, suppresses CNOT6L expression to exhibit antiproliferative activity

FEBS Open Bio
Despite the increasing evidence of the role of CCR4‐NOT complex in posttranscriptional gene regulation, relatively little is known about its mode of action. In a search for novel CCR4‐NOT interacting partners, we carried out mass spectrometry analysis of
Shou Soeda, Melissa Montrose, Akinori Takahashi, Risa Ishida, Sandrine Burriel, Nao Ohmine, Tohru Natsume, Shungo Adachi, Minsoo Kim, Tadashi Yamamoto   +9 more
doaj   +1 more source