A functional deadenylation assay identifies human CUG-BP as a deadenylation factor. [PDF]
Biology of the Cell, 2003 International audienceCUG-BP is a human nuclear and cytoplasmic RNA-binding protein. A role in the control of alternative splicing has been reported, but to date no cytoplasmic function for this protein has been demonstrated.
Legagneux, Vincent +2 more
core +3 more sources
Phosphorylation-dependent tuning of mRNA deadenylation rates. [PDF]
Nat Struct Mol BiolShortening of messenger RNA poly(A) tails by the Ccr4-Not complex initiates mRNA decay and is a major determinant of gene regulation. RNA adaptors modulate the specificity of deadenylation by binding to Ccr4-Not through their intrinsically disordered ...
Stowell JAW +11 more
europepmc +19 more sources
RNA deadenylation complexes in development and diseases
Biochemistry and Cell Biology, 2022 RNA deadenylation, the process of shortening of the 3’ poly(A) tail of an RNA molecule, is one of the key steps of post-transcriptional regulation of gene expression in eukaryotic cells.
Vu, Ly Phuong +2 more
core +3 more sources
Embryo deadenylation element-dependent deadenylation is enhanced by a cis element containing AUU repeats. [PDF]
Molecular and Cellular Biology, 1998 International audienceThe deadenylation of maternal mRNAs in the Xenopus embryo is a sequence-specific process. One cis element that targets maternal mRNAs for deadenylation after fertilization is the embryo deadenylation element (EDEN).
Audic, Yann +2 more
core +3 more sources
microRNA-mediated messenger RNA deadenylation contributes to translational repression in mammalian cells. [PDF]
PLoS ONE, 2009 Animal microRNAs (miRNAs) typically regulate gene expression by binding to partially complementary target sites in the 3' untranslated region (UTR) of messenger RNA (mRNA) reducing its translation and stability.
Traude H Beilharz +6 more
doaj +2 more sources
Molecular Insights into mRNA Polyadenylation and Deadenylation
International Journal of Molecular Sciences, 2022 Poly(A) tails are present on almost all eukaryotic mRNAs, and play critical roles in mRNA stability, nuclear export, and translation efficiency. The biosynthesis and shortening of a poly(A) tail are regulated by large multiprotein complexes. However, the
Xubing Lu +9 more
core +3 more sources
Cytoplasmic deadenylation: regulation of mRNA fate [PDF]
Biochemical Society Transactions, 2010 The poly(A) tail of mRNA has an important influence on the dynamics of gene expression. On one hand, it promotes enhanced mRNA stability to allow production of the protein, even after inactivation of transcription.
Lori A. Passmore, Katrin Wiederhold
core +3 more sources
CUG-BP and 3'UTR sequences influence PARN-mediated deadenylation in mammalian cell extracts
Genetics and Molecular Biology, 2007 Several mRNAs have been shown to exhibit distinct patterns of poly(A) shortening prior to their decay in vivo. In this study, we show that individual transcripts also demonstrate distinct patterns of deadenylation in in vitro systems derived from HeLa ...
Karen C.M. Moraes +2 more
doaj +2 more sources
Deadenylation is a widespread effect of miRNA regulation. [PDF]
RNA, 2008 miRNAs silence gene expression by repressing translation and/or by promoting mRNA decay. In animal cells, degradation of partially complementary miRNA targets occurs via deadenylation by the CAF1-CCR4-NOT1 deadenylase complex, followed by decapping and ...
E. Huntzinger +23 more
core +4 more sources
c-Jun ARE targets mRNA deadenylation by an EDEN-BP (embryo deadenylation element-binding protein)-dependent pathway. [PDF]
Journal of Biological Chemistry, 2002 International audienceIn mammalian cells, certain mRNAs encoding cytokines or proto-oncogenes are especially unstable, because of the presence of a particular sequence element in their 3'-untranslated region named ARE (A/U-rich element).
Legagneux, Vincent +3 more
core +4 more sources