Results 21 to 30 of about 14,150 (193)

The Dynamic Poly(A) Tail Acts as a Signal Hub in mRNA Metabolism

Cells, 2023
In eukaryotes, mRNA metabolism requires a sophisticated signaling system. Recent studies have suggested that polyadenylate tail may play a vital role in such a system.
Guiying Zhang, Haolin Luo, Xinyi Li, Zhangli Hu, Quan Wang   +4 more
doaj   +1 more source

A functional deadenylation assay identifies human CUG‐BP as a deadenylation factor [PDF]

Biology of the Cell, 2003
Abstract CUG‐BP is a human nuclear and cytoplasmic RNA‐binding protein. A role in the control of alternative splicing has been reported, but to date no cytoplasmic function for this protein has been demonstrated. A close sequence homolog of CUG‐BP is EDEN‐BP that is required for the specific cytoplasmic poly(A) tail shortening of certain mRNAs after ...
Paillard, Luc, Legagneux, Vincent, Osborne, Howard Beverley   +2 more
openaire   +2 more sources

Deadenylation and P-Bodies [PDF]

, 2012
Deadenylation is the major step in triggering mRNA decay and results in mRNA translation inhibition in eukaryotic cells. Therefore, it is plausible that deadenylation also induces the mRNP remodeling required for formation of GW bodies or RNA processing bodies (P-bodies), which harbor translationally silenced mRNPs.
Chyi-Ying A, Chen, Ann-Bin, Shyu
openaire   +2 more sources

Cap-dependent deadenylation of mRNA [PDF]

The EMBO Journal, 2000
Poly(A) tail removal is often the initial and rate-limiting step in mRNA decay and is also responsible for translational silencing of maternal mRNAs during oocyte maturation and early development. Here we report that deadenylation in HeLa cell extracts and by a purified mammalian poly(A)-specific exoribonuclease, PARN (previously designated ...
E, Dehlin, M, Wormington, C G, Körner, E, Wahle   +3 more
openaire   +2 more sources

A novel class of microRNA-recognition elements that function only within open reading frames. [PDF]

, 2018
MicroRNAs (miRNAs) are well known to target 3' untranslated regions (3' UTRs) in mRNAs, thereby silencing gene expression at the post-transcriptional level.
A Aizer, A Helwak, A Jakymiw, A Lal, AE Pasquinelli, AJ Giraldez, Alexander Dobin, AM Duursma, AR Buskirk, AR Quinlan, B Langmead, Bing Zhou, CJ Decker, CJ Shoemaker, CJ Woolstenhulme, CP Petersen, Dehe Wang, DP Bartel, DT Humphreys, E Huntzinger, EL Nostrand Van, GB Loeb, Guangqiao Liu, H Guo, HA Meijer, HO Iwakawa, I Behm-Ansmant, I Elcheva, J Hausser, J Liu, J Liu, JH Kim, Jie Zhou, JJ Forman, JP Broughton, K Seggerson, KA O’Donnell, Kai Zhang, L Wu, Ligang Wu, M Hafner, M Kouwenhove van, M Rehmsmeier, MD Robinson, MK Doma, ML Donnelly, N Rajewsky, NT Schirle, PA Maroney, Qian Zhao, R Ishimura, R Ishimura, R Parker, Ran Cao, RS Pillai, S Bagga, S Djuranovic, S Grosswendt, S Gu, S Jonas, S Nottrott, S Takagi, SN Bhattacharyya, SW Chi, SW Eichhorn, Timothy Nilsen, TT Tat, V Presnyak, W Filipowicz, Wen Ruan, X Adiconis, Xiang-Dong Fu, Xiaorong Zhang, Y Ishigaki, Y Liao, Y Tay, Y Wang, Y Xue, Y Zhao, Ya Zhao, Yan Qin, Yu Zhou, Yuanchao Xue, Z Cai, Zhiqiang Cai, Zonggui Chen   +85 more
core   +2 more sources

The 5' → 3' exoribonuclease XRN1/Pacman and its functions in cellular processes and development [PDF]

, 2012
XRN1 is a 5' → 3' processive exoribonuclease that degrades mRNAs after they have been decapped. It is highly conserved in all eukaryotes, including homologs in Drosophila melanogaster (Pacman), Caenorhabditis elegans (XRN1), and Saccharomyces cerevisiae (
Jones, Christopher Iain, Newbury, Sarah Faith, Zabolotskaya, Maria Vasilyevna   +2 more
core   +1 more source

MicroRNAs direct rapid deadenylation of mRNA [PDF]

Proceedings of the National Academy of Sciences, 2006
MicroRNAs (miRNAs) are ubiquitous regulators of eukaryotic gene expression. In addition to repressing translation, miRNAs can down-regulate the concentration of mRNAs that contain elements to which they are imperfectly complementary. Using miR-125b and let-7 as representative miRNAs, we show that in mammalian cells this reduction in message abundance ...
Ligang, Wu, Jihua, Fan, Joel G, Belasco
openaire   +2 more sources

Viruses and the cellular RNA decay machinery. [PDF]

, 2010
The ability to control cellular and viral gene expression, either globally or selectively, is central to a successful viral infection, and it is also crucial for the host to respond and eradicate pathogens.
Gaglia, Marta, Glaunsinger, Britt
core   +1 more source

CNOT1 regulates circadian behaviour through Per2 mRNA decay in a deadenylation-dependent manner

RNA Biology, 2022
Circadian clocks are an endogenous internal timekeeping mechanism that drives the rhythmic expression of genes, controlling the 24 h oscillatory pattern in behaviour and physiology.
Haytham Mohamed Aly Mohamed, Akinori Takahashi, Saori Nishijima, Shungo Adachi, Iori Murai, Hitoshi Okamura, Tadashi Yamamoto   +6 more
doaj   +1 more source

The 5' Cap Epitranscriptome and Beyond: Natural and Engineered 5' Cap Modifications for Optimizing mRNA Therapeutics and Functional Studies. [PDF]

ChemMedChem
5′ Cap modifications can be introduced via enzymes or chemical synthesis. Natural and non‐natural 5′ cap structures affect mRNA properties, including translation (T), stability (S), and/or immunogenicity (I). Eukaryotic mRNAs made by in vitro transcription have emerged as medical modalities for vaccination and protein replacement therapy. The 5′ cap is
Dahm GC, Pieper M, Schepers H, Rentmeister A.   +3 more
europepmc   +2 more sources