Results 31 to 40 of about 2,071 (166)

Identification of substrates of palmitoyl protein thioesterase 1 highlights roles of depalmitoylation in disulfide bond formation and synaptic function.

PLoS Biology, 2022
Loss-of-function mutations in the depalmitoylating enzyme palmitoyl protein thioesterase 1 (PPT1) cause neuronal ceroid lipofuscinosis (NCL), a devastating neurodegenerative disease.
Erica L Gorenberg, Sofia Massaro Tieze, Betül Yücel, Helen R Zhao, Vicky Chou, Gregory S Wirak, Susumu Tomita, TuKiet T Lam, Sreeganga S Chandra   +8 more
doaj   +1 more source

Identification of PSD-95 Depalmitoylating Enzymes [PDF]

The Journal of Neuroscience, 2016
Postsynaptic density (PSD)-95, the most abundant postsynaptic scaffolding protein, plays a pivotal role in synapse development and function. Continuous palmitoylation cycles on PSD-95 are essential for its synaptic clustering and regulation of AMPA receptor function.
Norihiko, Yokoi, Yuko, Fukata, Atsushi, Sekiya, Tatsuro, Murakami, Kenta, Kobayashi, Masaki, Fukata   +5 more
openaire   +2 more sources

Direct depalmitoylation with PPT1 validates putative synaptic substrates.

, 2022
(A) (i) Schematic of PPT1-mediated depalmitoylation assay, in which solubilized synaptosomes are treated with recombinant mPPT1 or GFP generated from HEK293T cells during Acyl RAC (Fig 1Bii). (ii) Venn diagram of PPT1-mediated depalmitoylation assay (n =
Sreeganga S. Chandra (12329746), Vicky Chou (12329740), Helen R. Zhao (12329737), Sofia Massaro Tieze (12329731), TuKiet T. Lam (610361), Betül Yücel (12329734), Gregory S. Wirak (12329743), Susumu Tomita (6956876), Erica L. Gorenberg (12329728)   +8 more
core   +1 more source

MC1R depalmitoylation inhibition reveals a physiological role for pheomelanin. [PDF]

PNAS Nexus
Abstract Pheomelanin is a sulfur-containing pigment produced by melanocytes notably in individuals with red hair/fair skin and animals with orange integumentary structures. Pheomelanin is not photoprotective as the dark eumelanin, but is cytotoxic, being related to a high risk of melanoma independently of UV radiation.
Galván I, García-Guerra M, Araujo-Roque M.   +2 more
europepmc   +3 more sources

APT1-Mediated Depalmitoylation Regulates Hippocampal Synaptic Plasticity

The Journal of Neuroscience, 2022
Palmitoylation may be relevant to the processes of learning and memory, and even disorders, such as post-traumatic stress disorder and aging-related cognitive decline. However, underlying mechanisms of palmitoylation in these processes remain unclear. Herein, we used acyl-biotin exchange, coimmunoprecipitation and biotinylation assays, and behavioral ...
Zu-Cheng Shen, Zhi-Xuan Xia, Jian-Min Liu, Jie-Yan Zheng, Yu-Fei Luo, Han Yang, Meng-Die Li, Ting Cao, Hai-Ping Liu, Gui-Lin Jin, Hui-Hui Huang, Chang-Xi Yu, Jun Zhou   +12 more
openaire   +2 more sources

ABHD16A Negatively Regulates the Palmitoylation and Antiviral Function of IFITM Proteins

mBio, 2022
Interferon-inducible transmembrane (IFITM) proteins are small homologous proteins that are encoded by the interferon-stimulated genes (ISGs), which can be strongly induced by interferon (IFN) and provide resistance to invasion by a variety of viral ...
Xuemeng Shi, Xiaoling Li, Zhao Xu, Lingyi Shen, Yunyun Ding, Shuaiwu Chen, Lin Mao, Wei Liu, Jun Xu   +8 more
doaj   +1 more source

Pharmacological Inhibition of APT2 by Solasonine Promotes Ferroptosis Through Palmitoylation Modulation in Gallbladder Cancer. [PDF]

Phytother Res
Schematic illustration of SS‐induced ferroptosis through targeting APT2‐mediated STAT3 palmitoylation in GBC cells. ABSTRACT Gallbladder cancer (GBC), the most prevalent and lethal biliary tract malignancy, lacks effective therapeutic strategies. Solasonine (SS), a bioactive steroidal alkaloid derived from Solanum nigrum L, exhibits antitumor activity ...
Shi LQ, Zhao JW, Zhu JY, Zhao C, Yang ZY, Pan LJ, Ma B, Chen ZY, Wang ZY, Li CJ, Liu SL, Mei JW, Dong P.   +12 more
europepmc   +2 more sources

Palmitoylation and depalmitoylation defects [PDF]

, 2021
Palmitoylation describes the enzymatic attachment of a 16-carbon atom fatty acid to a target protein. Such lipidation events occur in all eukaryotes and can be of reversible (S-palmitoylation) or irreversible (N-palmitoylation) nature.
Hornemann, Thorsten
core  

ABHD17 proteins are novel protein depalmitoylases that regulate N-Ras palmitate turnover and subcellular localization

eLife, 2015
Dynamic changes in protein S-palmitoylation are critical for regulating protein localization and signaling. Only two enzymes - the acyl-protein thioesterases APT1 and APT2 – are known to catalyze palmitate removal from cytosolic cysteine residues.
David Tse Shen Lin, Elizabeth Conibear
doaj   +1 more source

Depalmitoylation of Ykt6 Prevents its Entry into the Multivesicular Body Pathway [PDF]

Traffic, 2008
The dually lipidated SNARE Ykt6 is found on intracellular membranes and in the cytosol. In this study, we show that Ykt6 localizes to the Golgi as well as endosomal and vacuolar membranes in vivo. The ability of Ykt6 to cycle between the cytosol and the membranes depends on the intramolecular interaction of the N‐terminal longin and C‐terminal SNARE ...
Christoph T A, Meiringer, Kathrin, Auffarth, Haitong, Hou, Christian, Ungermann   +3 more
openaire   +2 more sources