Results 11 to 20 of about 2,219,993 (156)

Real-time imaging of DNA loop extrusion by condensin. [PDF]

Science, 2018
It has been hypothesized that SMC protein complexes such as condensin and cohesin spatially organize chromosomes by extruding DNA into large loops. We directly visualized the formation and processive extension of DNA loops by yeast condensin in real time.
Ganji M, Shaltiel IA, Bisht S, Kim E, Kalichava A, Haering CH, Dekker C.   +6 more
europepmc   +9 more sources

Condensin-driven loop extrusion on supercoiled DNA [PDF]

Nature Structural & Molecular Biology, 2022
Condensin, a structural maintenance of chromosomes (SMC) complex, has been shown to be a molecular motor protein that organizes chromosomes by extruding loops of DNA.
Pradhan, B., van der Torre, J., Kim, E. ; https://orcid.org/, Dekker, C., Gonzelez, A.   +4 more
core   +7 more sources

Cohesin mediates DNA loop extrusion by a "swing and clamp" mechanism. [PDF]

Cell, 2021
Structural maintenance of chromosomes (SMC) complexes organize genome topology in all kingdoms of life and have been proposed to perform this function by DNA loop extrusion. How this process works is unknown. Here, we have analyzed how loop extrusion is mediated by human cohesin-NIPBL complexes, which enable chromatin folding in interphase cells.
Bauer BW, Davidson IF, Canena D, Wutz G, Tang W, Litos G, Horn S, Hinterdorfer P, Peters JM.   +8 more
europepmc   +4 more sources

Active Loop Extrusion guides DNA-Protein Condensation [PDF]

Physical Review Letters
The spatial organization of DNA involves DNA loop extrusion and the formation of protein-DNA condensates. While the significance of each process is increasingly recognized, their interplay remains unexplored.
Jülicher, Frank, Takaki, Ryota, Brugués, Jan, Savich, Yahor   +3 more
core   +7 more sources

Two CTCF motifs impede cohesin-mediated DNA loop extrusion

Molecular Cell
Cohesin extrudes DNA into loops and is positioned along the genome by stalling at CTCF upon encountering its N-terminal region (NTR). The mechanism underlying this stalling, however, is unresolved. Using single-molecule assays that monitor DNA loop extrusion (LE) in the presence of NTR fragments, we identify two amino acid motifs, YDF and KTYQR, that ...
Barth R, Janissen R, Muras L, van der Torre J, Litos G, van der Sluis E, van der Graaf A, Davidson IF, Peters J, Dekker C.   +9 more
europepmc   +5 more sources

Theory and simulations of condensin mediated loop extrusion in DNA [PDF]

Nature Communications, 2021
How chromosomes, which are polymers with nearly billion base pairs, are packaged in the restricted nuclear volume is not well understood. Here, the authors combine polymer physics, nonequilibrium fluctuation theorem, and simulations to quantitatively ...
Ryota Takaki, Atreya Dey, Guang Shi, D. Thirumalai   +3 more
doaj   +5 more sources

Chromosome organization by one-sided and two-sided loop extrusion

eLife, 2020
SMC complexes, such as condensin or cohesin, organize chromatin throughout the cell cycle by a process known as loop extrusion. SMC complexes reel in DNA, extruding and progressively growing DNA loops.
Edward J Banigan, Aafke A van den Berg, Hugo B Brandão, John F Marko, Leonid A Mirny   +4 more
doaj   +2 more sources

NIPBL and STAG1 enable loop extrusion by providing differential DNA-cohesin affinity [PDF]

Proceedings of the National Academy of Sciences
DNA loop extrusion by cohesin has emerged as a critical pathway for chromosome organization. In vitro single-molecule experiments indicate that loop extrusion requires the assembly of a heteropentameric complex consisting of the SMC1/SMC3 heterodimer ...
Raman van Wee, Roi Asor, Yiwen Li, David Drechsel, Mariia Popova, Gabriele Litos, Iain F. Davidson, Jan-Michael Peters, Philipp Kukura   +8 more
core   +5 more sources

In vitro dynamics of DNA loop extrusion by structural maintenance of chromosomes complexes [PDF]

Current Opinion in Genetics & Development
Genomic DNA inside the cell's nucleus is highly organized and tightly controlled by the structural maintenance of chromosomes (SMC) protein complexes.
Rutkauskas, M., Kim, E. ; https://orcid.org/   +1 more
core   +4 more sources

Active loop extrusion modulates the mechanical response of chromatin under tension

Physical Review Research
Chromosomes are complex biopolymers folded into dynamic loops via a loop-extrusion process and may experience various mechanical forces in vivo. We develop a force-dependent model of chromatin loop extrusion and investigate its mechanical consequences on
Hossein Salari, Daniel Jost
doaj   +2 more sources