Results 11 to 20 of about 9,982 (202)
Real-time imaging of DNA loop extrusion by condensin [PDF]
Science, 2018 Live imaging of DNA loop extrusion To spatially organize chromosomes, ring-shaped protein complexes including condensin and cohesin have been hypothesized to extrude DNA loops. Condensin has been shown to exhibit a DNA-translocating motor function, but extrusion has not been observed directly.
Cees Dekker +2 more
exaly +8 more sources
Two CTCF motifs impede cohesin-mediated DNA loop extrusion
Molecular CellCohesin extrudes DNA into loops and is positioned along the genome by stalling at CTCF upon encountering its N-terminal region (NTR). The mechanism underlying this stalling, however, is unresolved. Using single-molecule assays that monitor DNA loop extrusion (LE) in the presence of NTR fragments, we identify two amino acid motifs, YDF and KTYQR, that ...
Cees Dekker +2 more
exaly +5 more sources
Theory and simulations of condensin mediated loop extrusion in DNA [PDF]
Nature Communications, 2021 How chromosomes, which are polymers with nearly billion base pairs, are packaged in the restricted nuclear volume is not well understood. Here, the authors combine polymer physics, nonequilibrium fluctuation theorem, and simulations to quantitatively ...
Ryota Takaki +3 more
doaj +5 more sources
Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phase. [PDF]
EMBO J, 2023 Abstract Genetic information is stored in linear DNA molecules, which are highly folded inside cells. DNA replication along the folded template path yields two sister chromatids that initially occupy the same nuclear region in an intertwined arrangement.
Batty P +6 more
europepmc +4 more sources
Cornelia de Lange syndrome mutations in NIPBL can impair cohesin-mediated DNA loop extrusion [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 2022 Alexander Schleiffer +2 more
exaly +2 more sources
Structural insights into DNA loop extrusion by SMC protein complexes. [PDF]
Curr Opin Struct Biol, 2020 Structural Maintenance of Chromosomes (SMC) protein complexes play key roles in the three-dimensional organization of genomes in all kingdoms of life. Recent insights from chromosome contact mapping experiments and single-molecule imaging assays suggest that these complexes achieve distinct cellular functions by extruding large loops of DNA while they ...
Datta S, Lecomte L, Haering CH.
europepmc +4 more sources
Condensin-driven loop extrusion on supercoiled DNA [PDF]
Nature Structural & Molecular Biology, 2021 Abstract Condensin, a structural maintenance of chromosomes (SMC) complex, has been shown to be a molecular motor protein that organizes chromosomes by extruding loops of DNA. In cells, such loop extrusion is challenged by many potential conflicts, e.g., the torsional stresses that are generated by other DNA-processing enzymes.
Eugene Kim +4 more
openaire +5 more sources
Human cohesin compacts DNA by loop extrusion [PDF]
Science, 2019 Cohesin extrudes DNA loops DNA is folded into loops in eukaryotic cells by a process that depends on a ring-shaped adenosine triphosphatase complex called cohesin. Davidson et al. and Kim et al.
Yoori Kim +4 more
openaire +2 more sources
Cohesin and condensin extrude DNA loops in a cell cycle-dependent manner
eLife, 2020 Loop extrusion by structural maintenance of chromosomes (SMC) complexes has been proposed as a mechanism to organize chromatin in interphase and metaphase. However, the requirements for chromatin organization in these cell cycle phases are different, and
Stefan Golfier +3 more
doaj +1 more source
Epigenetics & Chromatin, 2021 Cohesin is a key organizer of chromatin folding in eukaryotic cells. The two main activities of this ring-shaped protein complex are the maintenance of sister chromatid cohesion and the establishment of long-range DNA–DNA interactions through the process
Arkadiy K. Golov +3 more
doaj +1 more source