Loop extrusion as a mechanism for formation of DNA damage repair foci
Nature, 2021 The repair of DNA double-strand breaks (DSBs) is essential for safeguarding genome integrity. When a DSB forms, the PI3K-related ATM kinase rapidly triggers the establishment of megabase-sized, chromatin domains decorated with phosphorylated histone H2AX (γH2AX), which act as seeds for the formation of DNA-damage response foci1. It is unclear how these
Raphaël Mourad +2 more
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CTCF is a DNA-tension-dependent barrier to cohesin-mediated DNA loop extrusion
, 2022 Abstract In eukaryotes, genomic DNA is extruded into loops by cohesin 1 . By restraining this process, the DNA-binding protein CTCF generates topologically associating domains (TADs) 2-4 that play key roles in gene regulation and recombination ...
Davidson IF +10 more
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Condensin loop extrusion properties, roadblocks, and role in homology search during recombination in S. cerevisiae [PDF]
The EMBO JournalThe in vivo mechanism, cis-acting roadblocks, and biological functions of DNA loop extrusion by eukaryotic SMC complexes remain incompletely defined. Here, we identify condensin-dependent Hi-C contact stripes at the recombination enhancer (RE) and at ...
Vinciane Piveteau +6 more
doaj +2 more sources
DNA actively regulates the “safety-belt” dynamics of condensin during loop extrusion
Nature CommunicationsCondensin plays an essential role in genome folding through its active DNA loop extrusion activity. Condensin contains a binding interface between its Ycg1 HEAT-repeat subunit and the Brn1 kleisin, together forming a “safety-belt” DNA-binding groove ...
Jinyu Chen +3 more
doaj +3 more sources
Cornelia de Lange syndrome mutations in NIPBL can impair cohesin-mediated DNA loop extrusion [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 2022 Alexander Schleiffer +2 more
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NIPBL and STAG1 enable loop extrusion by providing differential DNA-cohesin affinity [PDF]
Proceedings of the National Academy of SciencesAbstract DNA loop extrusion by cohesin has emerged as a critical pathway for chromosome organization. In vitro single-molecule experiments indicate that loop extrusion requires the assembly of an heteropentameric complex consisting of the SMC1/SMC3 heterodimer, STAG1, NIPBL and the kleisin SCC1. The multimeric nature of this complex and
Raman van Wee +8 more
openaire +5 more sources
Author Correction: Testing pseudotopological and nontopological models for SMC-driven DNA loop extrusion against roadblock-traversal experiments [PDF]
Scientific Reports, 2023 Roman Barth +6 more
doaj +2 more sources
STAG2-RAD21 complex: A unidirectional DNA ratchet mechanism in loop extrusion
International Journal of Biological MacromoleculesDNA loop extrusion plays a key role in the regulation of gene expression and the structural arrangement of chromatin. Most existing mechanistic models of loop extrusion depend on some type of ratchet mechanism, which should permit the elongation of loops while preventing their collapse, by enabling DNA to move in only one direction.
Inigo Marcos-Alcalde +2 more
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Fluidification of entangled polymers by loop extrusion [PDF]
Physical Review ResearchLoop extrusion is one of the main processes shaping chromosome organization across the cell cycle, yet its role in regulating deoxyribonucleic acid (DNA) entanglement and nucleoplasm viscoelasticity remains overlooked.
Filippo Conforto +2 more
doaj +2 more sources
Numerical modelling of the aluminium extrusion process [PDF]
The extrusion of aluminium alloys involves the shaping of the product from an homogenised billet into a complex shape. In addition the properties of the extrudate are closely related to the processing parameters (temperature, stain rate, and material ...
Peng, Z.
core +9 more sources