Results 21 to 30 of about 2,219,993 (156)

DNA actively regulates the “safety-belt” dynamics of condensin during loop extrusion

Nature Communications
Condensin plays an essential role in genome folding through its active DNA loop extrusion activity. Condensin contains a binding interface between its Ycg1 HEAT-repeat subunit and the Brn1 kleisin, together forming a “safety-belt” DNA-binding groove ...
Jinyu Chen, Cibo Feng, Yong Wang, Xiakun Chu   +3 more
doaj   +3 more sources

Fluidification of entangled polymers by loop extrusion [PDF]

Physical Review Research
Loop extrusion is one of the main processes shaping chromosome organization across the cell cycle, yet its role in regulating deoxyribonucleic acid (DNA) entanglement and nucleoplasm viscoelasticity remains overlooked.
Filippo Conforto, Yair Gutierrez Fosado, Davide Michieletto   +2 more
doaj   +2 more sources

Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phase. [PDF]

EMBO J, 2023
Abstract Genetic information is stored in linear DNA molecules, which are highly folded inside cells. DNA replication along the folded template path yields two sister chromatids that initially occupy the same nuclear region in an intertwined arrangement.
Batty P, Langer CC, Takács Z, Tang W, Blaukopf C, Peters JM, Gerlich DW.   +6 more
europepmc   +4 more sources

An extrinsic motor directs chromatin loop formation by cohesin

The EMBO Journal
The ring-shaped cohesin complex topologically entraps two DNA molecules to establish sister chromatid cohesion. Cohesin also shapes the interphase chromatin landscape with wide-ranging implications for gene regulation, and cohesin is thought to achieve ...
Thomas M Guérin, Christopher Barrington, Georgii Pobegalov, Maxim I Molodtsov, Frank Uhlmann   +4 more
doaj   +2 more sources

Cornelia de Lange syndrome mutations in NIPBL can impair cohesin-mediated DNA loop extrusion. [PDF]

Proc Natl Acad Sci U S A, 2022
Panarotto M, Davidson IF, Litos G, Schleiffer A, Peters JM.   +4 more
europepmc   +2 more sources

Structural insights into DNA loop extrusion by SMC protein complexes. [PDF]

Curr Opin Struct Biol, 2020
Structural Maintenance of Chromosomes (SMC) protein complexes play key roles in the three-dimensional organization of genomes in all kingdoms of life. Recent insights from chromosome contact mapping experiments and single-molecule imaging assays suggest that these complexes achieve distinct cellular functions by extruding large loops of DNA while they ...
Datta S, Lecomte L, Haering CH.
europepmc   +4 more sources

Cohesin and condensin extrude DNA loops in a cell cycle-dependent manner

eLife, 2020
Loop extrusion by structural maintenance of chromosomes (SMC) complexes has been proposed as a mechanism to organize chromatin in interphase and metaphase. However, the requirements for chromatin organization in these cell cycle phases are different, and
Stefan Golfier, Thomas Quail, Hiroshi Kimura, Jan Brugués   +3 more
doaj   +1 more source

Sensitivity of cohesin–chromatin association to high-salt treatment corroborates non-topological mode of loop extrusion

Epigenetics & Chromatin, 2021
Cohesin is a key organizer of chromatin folding in eukaryotic cells. The two main activities of this ring-shaped protein complex are the maintenance of sister chromatid cohesion and the establishment of long-range DNA–DNA interactions through the process
Arkadiy K. Golov, Anastasia V. Golova, Alexey A. Gavrilov, Sergey V. Razin   +3 more
doaj   +1 more source

CTCF is a DNA-tension-dependent barrier to cohesin-mediated DNA loop extrusion

, 2022
Abstract In eukaryotes, genomic DNA is extruded into loops by cohesin 1 . By restraining this process, the DNA-binding protein CTCF generates topologically associating domains (TADs) 2-4 that play key roles in gene regulation and recombination ...
Davidson IF, Barth R, Zaczek M, van der Torre J, Tang W, Nagasaka K, Janissen R, Kerssemakers J, Wutz G, Dekker C, Peters J.   +10 more
europepmc   +2 more sources

The Smc5/6 complex is a DNA loop-extruding motor [PDF]

, 2023
Structural maintenance of chromosomes (SMC) protein complexes are essential for the spatial organization of chromosomes1. Whereas cohesin and condensin organize chromosomes by extrusion of DNA loops, the molecular functions of the third eukaryotic SMC ...
Kim, E. ; https://orcid.org/, Loke, M., Kanno, T., Umeda Igarashi, M., Jeppsson, K., Björkegren, C., Baaske, M. ; https://orcid.org/, Pradhan, B. ; https://orcid.org/, Wong, J. ; https://orcid.org/   +8 more
core   +1 more source