Results 91 to 100 of about 1,452,122 (130)

HU of Streptococcus pneumoniae Is Essential for the Preservation of DNA Supercoiling

Frontiers in Microbiology, 2018
The histone-like protein HU is a conserved nucleoid-associated protein that is involved in the maintenance of the bacterial chromosome architecture. It is the only known nucleoid-associated protein in Streptococcus pneumoniae, but it has not been studied.
María-José Ferrándiz, David Carreño, Silvia Ayora, Adela G. de la Campa, Adela G. de la Campa   +4 more
doaj   +1 more source

Phytochemical Profiling and Antibacterial Activity of Moroccan Monofloral Honeys Against Multidrug‐Resistant Uropathogens: In Vitro, ADMET, Molecular Docking, and Molecular Dynamics Approaches

Food Science &Nutrition, Volume 14, Issue 9, September 2026.
Multidrug‐resistant bacteria infections require alternative antimicrobial strategies. This study evaluated the phenolic composition, antibacterial activity, and in silico properties of four Moroccan monofloral honeys. Carob honey showed the greatest phenolic diversity, whereas citrus honey contained the lowest polyphenolic content.
Mourad Chikhaoui, Aziz Galman, Ahmed Chetoui, Oussama Khibech, Mohamed Reda Kachmar, Toufik Bouddine, Mohamed Bouhrim, Fahd A. Nasr, Mohammed Al‐Zharani, Ashraf Ahmed Qurtam, Rachid Lotfi, Houda El Hajjouji   +11 more
wiley   +1 more source

DNA supercoiling enhances DNA condensation by ParB proteins.

The ParABS system plays a critical role in bacterial chromosome segregation. The key component of this system, ParB, loads and spreads along DNA to form a local protein-DNA condensate known as a partition complex.
Kemps, G., Dekker, C., Barth, A., Tišma, M., Antar, H., Janissen, R., Gruber, S., Martin-Gonzalez, A., Analikwu, B.T.   +8 more
core   +1 more source

Integrated one‐pot CRISPR‐Cas detection: Addressing strategies and future prospects

Interdisciplinary Medicine, Volume 4, Issue 5, September 2026.
Integrated one‐pot CRISPR‐Cas detection significantly simplifies workflows, reduces cross‐contamination risks, and shortens turnaround times, making it highly suitable for point‐of‐care testing (POCT) and resource‐limited settings. Key challenges include system compatibility issues between Cas proteins and isothermal amplification, limited multiplexing
Jian Zhou, Xuemei Ren, Yingjie Ren, Ruiping Wu, Ruiman Geng, Le Chen, Zhuo Li   +6 more
wiley   +1 more source

Effects of physiological self-crowding of DNA on shape and biological properties of DNA molecules with various levels of supercoiling [PDF]

, 2015
DNA in bacterial chromosomes and bacterial plasmids is supercoiled. DNA supercoiling is essential for DNA replication and gene regulation. However, the density of supercoiling in vivo is circa twice smaller than in deproteinized DNA molecules isolated ...
Kwapich, R., Japaridze, Aleksandre, Benedetti, F., Burnier, Yannis, Stasiak, Andrzej, Dorier, Julien, Stasiak, A., Benedetti, Fabrizio, Dorier, J., Burnier, Y., Racko, Dusan, Dietler, Giovanni, Japaridze, A., Kwapich, Robert, Racko, D., Dietler, G.   +15 more
core   +2 more sources

Conformational diversity in poly‐HAMP arrays and its implications for signal transduction

Protein Science, Volume 35, Issue 9, September 2026.
Abstract Prokaryotic transmembrane receptors are built around a helical coiled‐coil backbone, with sensory, modulatory, and effector domains arranged along its length. The modulatory HAMP domain forms a parallel four‐helix coiled coil integrated into this backbone, typically connecting transmembrane segments with downstream cytosolic domains.
Murray Coles, Carolin P. Ewers, Reinhard Albrecht, Mikel Martinez‐Goikoetxea, Malgorzata Orlowska, Jörg Martin, Andrei N. Lupas, Marcus D. Hartmann, Stanislaw Dunin‐Horkawicz   +8 more
wiley   +1 more source

Cinnamic‐Hydroxamic‐Acid Derivatives Exhibit Antibiotic, Anti‐Biofilm, and Supercoiling Relaxation Properties by Targeting Bacterial Nucleoid‐Associated Protein HU

Advanced Science
Finding novel compounds and drug targets is crucial for antibiotic development. The nucleoid‐associated protein HU plays a significant role in bacterial DNA metabolism, supercoiling, and biofilm formation, making it a promising new target.
Huan Chen, Yingqi Xu, Zhaoping Xiong, Hongliang Wang, Xin Wang, Yue Kang, Zhonglin Wang, Xiaoyan Zeng, Yahui Liu, Yehuan Zheng, Wei Chen, Mengzhe Li, Zhenyue Hu, Chi Xu, Yue Wu, Yawen Wang, Zuyi Yuan, Shuai Yuan, Huadong Liu, Steve Matthews, Nan Qiao, Yan Li, Bing Liu   +22 more
doaj   +1 more source

Structural and dynamic basis of a supercoiling-responsive DNA element

, 2006
In both eukaryotes and prokaryotes, negative supercoiling of chromosomal DNA acts locally to regulate a variety of cellular processes, such as transcription, replication, recombination and response to environmental stresses.
Lim, HM, Bae, SH, Sun, DW, Choi, Byong-Seok, Yun, SH   +4 more
core   +1 more source

ATP-dependent DNA supercoiling assay.

, 2016
Supercoiling activities of DNA gyrases composed of WT-GyrA, Gly89Cys-GyrA, Ala91Val-GyrA or Asp95Gly-GyrA and WT-GyrB were confirmed. Relaxed pBR322 DNA was incubated with GyrA, GyrB, or both, of the subunits in the presence or absence of ATP.
Kazumasa Yokoyama (127298), Chie Nakajima (127312), Tomoyuki Yamaguchi (150399), Yasuhiko Suzuki (378)   +3 more
core   +1 more source

Role of Supercoiling and Topoisomerases in DNA Knotting

DNA
DNA knots are deleterious for living cells if not removed. Several theoretical and simulation approaches address the question of how topoisomerases select the intermolecular passages that preferentially lead to unknotting rather than to the knotting of ...
Jorge Cebrián, María-Luisa Martínez-Robles, Victor Martínez, Pablo Hernández, Dora B. Krimer, Jorge B. Schvartzman, María-José Fernández-Nestosa   +6 more
doaj   +1 more source