Results 11 to 20 of about 8,858 (210)

A novel membrane stress response that blocks chromosomal replication by targeting the DnaA initiator via the ClpP protease [PDF]

Journal of Bacteriology
In Escherichia coli, membrane stress due to interrupted lipoprotein (Lpp) maturation impairs DNA replication and arrests cell growth. How Lpp maturation and DNA replication are connected remains unclear.
Alabi Gbolahan, Tong Li, Rishit Saxena, Karen Wolcott, Aamna Sohail, Ishika Ahmed, Dhruba K. Chattoraj, Elliott Crooke, Rahul Saxena   +8 more
doaj   +2 more sources

Mutations of DnaA-boxes in the oriR region increase replication frequency of the MiniR1–1 plasmid

BMC Microbiology, 2018
Background The MiniR1–1 plasmid is a derivative of the R1 plasmid, a low copy cloning vector. Results Nucleotide sequencing analysis shows that the MiniR1–1 plasmid is a 6316 bp circular double-stranded DNA molecule with an oriR1 (origin for replication).
Yuan Yao, Sukhbold Enkhtsetseg, Ingvild Odsbu, Lifei Fan, Morigen Morigen   +4 more
doaj   +2 more sources

DnaA and LexA Proteins Regulate Transcription of the uvrB Gene in Escherichia coli: The Role of DnaA in the Control of the SOS Regulon

Frontiers in Microbiology, 2018
The uvrB gene belongs to the SOS network, encoding a key component of the nucleotide excision repair. The uvrB promoter region contains three identified promoters with four LexA binding sites, one consensus and six potential DnaA binding sites.
Wurihan, Gezi, Elisa Brambilla, Shuwen Wang, Hongwei Sun, Lifei Fan, Yixin Shi, Yixin Shi, Bianca Sclavi, Morigen   +9 more
doaj   +2 more sources

Real-time analysis of initiation regulation systems during the progression of the reconstituted chromosomal replication cycle [PDF]

Scientific Reports
Chromosome replication in Escherichia coli is primarily regulated at the initiation stage, where the DnaA protein activates replication at the chromosomal origin, oriC.
Koki Kanoh, Masayuki Su’etsugu
doaj   +2 more sources

Dominance of dnaA+ to dnaA in Escherichia coli [PDF]

Journal of Bacteriology, 1977
The dominance of dnaA+ to the dnaA508 mutation was complete and was unaffected by the presence of a copy of the chromosomal replication origin on the episome. These results prove that the dnaA gene of Escherichia coli produces a diffusible product.
F, Gotfried, J A, Wechsler
openaire   +2 more sources

Putative Cooperative ATP-DnaA Binding to Double-Stranded DnaA Box and Single-Stranded DnaA-Trio Motif upon Helicobacter pylori Replication Initiation Complex Assembly [PDF]

, 2021
oriC is a region of the bacterial chromosome at which the initiator protein DnaA interacts with specific sequences, leading to DNA unwinding and the initiation of chromosome replication.
Weigel, C., Pawel Jaworski, Rafal Donczew, Zyla-Uklejewicz, D., Nowaczyk-Cieszewska, M., Zawilak-Pawlik, A., Christoph Weigel, Anna Zawilak-Pawlik, Thorsten Mielke, Mielke, T., Jaworski, P., Malgorzata Nowaczyk-Cieszewska, Dorota Zyla-Uklejewicz, Donczew, R.   +13 more
core   +1 more source

Cooperative DnaA Binding to the Negatively Supercoiled datA Locus Stimulates DnaA-ATP Hydrolysis

Cooperative DnaA Binding to the Negatively Supercoiled datA Locus Stimulates DnaA-ATP Hydrolysis
Timely initiation of replication in Escherichia coli requires functional regulation of the replication initiator, ATP-DnaA. The cellular level of ATP-DnaA increases just before initiation, after which its level decreases through hydrolysis of DnaA-bound ATP, yielding initiation-inactive ADP-DnaA.
Sakai, Ryuji, Kasho, Kazutoshi, Tanaka, Hiroyuki, タナカ, ヒロユキ, 田中, 宏幸, 酒井, 隆至, カタヤマ, ツトム, カショウ, カズトシ, 加生, 和寿, 片山, 勉, サカイ, リュウジ, Katayama, Tsutomu   +11 more
openaire   +1 more source

Inactivation of Individual SeqA Binding Sites of the E. coli Origin Reveals Robustness of Replication Initiation Synchrony. [PDF]

PLoS ONE, 2016
The Escherichia coli origin of replication, oriC, comprises mostly binding sites of two proteins: DnaA, a positive regulator, and SeqA, a negative regulator.
Jyoti K Jha, Dhruba K Chattoraj
doaj   +1 more source

Evolutionary Changes in DnaA-Dependent Chromosomal Replication in Cyanobacteria

Frontiers in Microbiology, 2020
Replication of the circular bacterial chromosome is initiated at a unique origin (oriC) in a DnaA-dependent manner in which replication proceeds bidirectionally from oriC to ter. The nucleotide compositions of most bacteria differ between the leading and
Ryudo Ohbayashi, Shunsuke Hirooka, Ryo Onuma, Yu Kanesaki, Yuu Hirose, Yusuke Kobayashi, Takayuki Fujiwara, Takayuki Fujiwara, Chikara Furusawa, Chikara Furusawa, Shin-ya Miyagishima, Shin-ya Miyagishima   +11 more
doaj   +1 more source

Conserved hydrophobic amino acid residues in the N-terminal region of DnaA protein are involved in DnaA–DnaA interaction [PDF]

Biochemical Journal, 2002
We previously reported that a leucine-zipper-like structure (I26, L33 and L40) located in the N-terminal region of DnaA is essential for the duplex opening at oriC by DnaA. In this study, we focused on three other conserved hydrophobic amino acid residues, L3, L10 and L17, and examined the function of DnaA proteins mutated in these amino acid residues.
Shinji, Mima, Masaki, Makise, Motohiro, Koterasawa, Tomofusa, Tsuchiya, Tohru, Mizushima   +4 more
openaire   +2 more sources