Results 31 to 40 of about 9,669 (211)
Changing Perspectives on the Role of DnaA-ATP in Orisome Function and Timing Regulation
Frontiers in Microbiology, 2019 Bacteria, like all cells, must precisely duplicate their genomes before they divide. Regulation of this critical process focuses on forming a pre-replicative nucleoprotein complex, termed the orisome.
Alan C. Leonard +3 more
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Escherichia coli DnaA Protein Loads a Single DnaB Helicase at a DnaA Box Hairpin [PDF]
Journal of Biological Chemistry, 2002 The molecular engine that drives bidirectional replication fork movement from the Escherichia coli replication origin (oriC) is the replicative helicase, DnaB. At oriC, two and only two helicase molecules are loaded, one for each replication fork. DnaA participates in helicase loading; DnaC is also involved, because it must be in a complex with DnaB ...
Kevin M, Carr, Jon M, Kaguni
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In-vitro helix opening of M. tuberculosis oriC by DnaA occurs at precise location and is inhibited by IciA like protein. [PDF]
PLoS ONE, 2009 BACKGROUND: Mycobacterium tuberculosis (M.tb), the pathogen that causes tuberculosis, is capable of staying asymptomatically in a latent form, persisting for years in very low replicating state, before getting reactivated to cause active infection. It is
Sandeep Kumar +2 more
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Avoiding chromosome pathology when replication forks collide [PDF]
, 2013 This is the author's accepted manuscript. The final published article is available from the link below. Copyright @ 2013 Macmillan Publishers Limited.Chromosome duplication normally initiates through the assembly of replication fork complexes at defined ...
Lloyd, RG +3 more
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Cooperative DnaA Binding to the Negatively Supercoiled datA Locus Stimulates DnaA-ATP Hydrolysis [PDF]
Journal of Biological Chemistry, 2017 Timely initiation of replication in Escherichia coli requires functional regulation of the replication initiator, ATP-DnaA. The cellular level of ATP-DnaA increases just before initiation, after which its level decreases through hydrolysis of DnaA-bound ATP, yielding initiation-inactive ADP-DnaA.
Kazutoshi, Kasho +3 more
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The EMBO Journal, 1989 The complex of DnaA protein with its 9 bp consensus binding site, the dnaA box 5'-TT(A/T)T(A/C)CA(A/C)A, blocks transcribing RNA polymerase. In a model system, the rate of transcription was monitored distal to the dnaA box 5'-TTTTCCACA by the expression of a reporter gene.
C, Schaefer, W, Messer
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DnaA and ORC: more than DNA replication initiators [PDF]
Trends in Cell Biology, 2011 Mutations in DNA replication initiator genes in both prokaryotes and eukaryotes lead to a pleiotropic array of phenotypes, including defects in chromosome segregation, cytokinesis, cell cycle regulation and gene expression. For years, it was not clear whether these diverse effects were indirect consequences of perturbed DNA replication, or whether they
Scholefield G, Veening JW, Murray H
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PLoS ONE, 2012 DnaA initiates chromosome replication in most known bacteria and its activity is controlled so that this event occurs only once every cell division cycle.
Tomer Regev +3 more
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Transcription termination in the Escherichia coli dnaA gene is not mediated by the internal DnaA box
Journal of Bacteriology, 1995 DnaA protein is a DNA-binding protein which recognizes a 9-bp consensus sequence called the DnaA box. By binding to DnaA boxes, DnaA protein regulates initiation of chromosomal replication and transcription of several genes. The dnaA gene contains two DnaA boxes, one located in the regulatory region and one within the structural gene. In this paper, we
I, Pérez-Roger +2 more
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Frontiers in Microbiology, 2018 Although the mechanisms that precisely time initiation of chromosome replication in bacteria remain unclear, most clock models are based on accumulation of the active initiator protein, DnaA-ATP.
Prassanna Rao +4 more
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