Results 21 to 30 of about 9,669 (211)
Conserved hydrophobic amino acid residues in the N-terminal region of DnaA protein are involved in DnaA–DnaA interaction [PDF]
Biochemical Journal, 2002 We previously reported that a leucine-zipper-like structure (I26, L33 and L40) located in the N-terminal region of DnaA is essential for the duplex opening at oriC by DnaA. In this study, we focused on three other conserved hydrophobic amino acid residues, L3, L10 and L17, and examined the function of DnaA proteins mutated in these amino acid residues.
Shinji, Mima +4 more
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The DnaA Protein Is Not the Limiting Factor for Initiation of Replication in Escherichia coli. [PDF]
PLoS Genetics, 2015 The bacterial replication cycle is driven by the DnaA protein which cycles between the active ATP-bound form and the inactive ADP-bound form. It has been suggested that DnaA also is the main controller of initiation frequency.
Ingvild Flåtten +4 more
doaj +1 more source
Rapid turnover of DnaA at replication origin regions contributes to initiation control of DNA replication. [PDF]
PLoS Genetics, 2017 DnaA is a conserved key regulator of replication initiation in bacteria, and is homologous to ORC proteins in archaea and in eukaryotic cells. The ATPase binds to several high affinity binding sites at the origin region and upon an unknown molecular ...
Katrin Schenk +8 more
doaj +1 more source
, 2022 oriC is a region of the bacterial chromosome at which the initiator protein DnaA interacts with specific sequences, leading to DNA unwinding and the initiation of chromosome replication.
Weigel, Christoph +6 more
core +1 more source
eLife, 2021 The ability to regulate DNA replication initiation in response to changing nutrient conditions is an important feature of most cell types. In bacteria, DNA replication is triggered by the initiator protein DnaA, which has long been suggested to respond ...
Michele Felletti +3 more
doaj +1 more source
Interaction of the Bacillus subtilis DnaA-like protein with the Escherichia coli DnaA protein [PDF]
Journal of Bacteriology, 1988 Plasmids carrying the intact Bacillus subtilis dnaA-like gene and two reciprocal hybrids between the B. subtilis and Escherichia coli dnaA genes were constructed. None of the plasmids could transform wild-type E. coli cells unless the cells contained surplus E. coli DnaA protein (DnaAEc).
L, Andrup +4 more
openaire +2 more sources
Titration of DnaA protein by oriC DnaA‐boxes increases dnaA gene expression in Escherichia coli.
The EMBO Journal, 1987 Binding of the DnaA protein to its binding sites, the DnaA-boxes (TTATCCACA), was measured by a simple physiological approach. The presence of extra DnaA-boxes in growing cells leads to a derepression of dnaA gene expression, measured as beta-galactosidase activity of a dnaA-lacZ fusion polypeptide.
F G, Hansen +3 more
openaire +2 more sources
Frontiers in Microbiology, 2018 Chromosomal replication initiation requires dynamic mechanisms in higher-order nucleoprotein complexes that are constructed at the origin of replication.
Yukari Sakiyama +5 more
doaj +1 more source
dnaA alleles are recessive [PDF]
Journal of Bacteriology, 1980 Dominance tests of several dnaA alleles from Escherichia coli, including two previously reported to be dominant, show that all of the mutant alleles examined are recessive to dnaA+.
openaire +2 more sources
Nutritional Control of DNA Replication Initiation through the Proteolysis and Regulated Translation of DnaA. [PDF]
PLoS Genetics, 2015 Bacteria can arrest their own growth and proliferation upon nutrient depletion and under various stressful conditions to ensure their survival. However, the molecular mechanisms responsible for suppressing growth and arresting the cell cycle under such ...
David J Leslie +7 more
doaj +1 more source