Results 61 to 70 of about 9,669 (211)
Lon-mediated DnaA proteolysis.
(A) DnaA in vivo stability assay of wild-type and lon::Ω cells in mid-exponential phase in M2G and while experiencing N or P starvation. (B) Quantification of DnaA in vivo stability assays done as shown in (A), from either five (M2G mid-exponential phase)
Julien Mortier (5692604) +5 more
core +1 more source
Abstract In bacteria, DnaB replicative helicases are essential for unwinding genomic DNA to provide the single‐stranded templates required for replication. The recruitment of the hexameric DnaB helicase to the origin of replication is mediated by distinct mechanisms that rely on specialized factors known as helicase loaders. The DciA family of helicase
Daniele Mazzoletti +8 more
wiley +1 more source
Despite the advantages of digital nucleic acid analysis (DNAA) in terms of sensitivity, precision, and resolution, current DNAA methods commonly suffer a limitation in multiplexing capacity.
Dongyang Cai +12 more
doaj +1 more source
DnaA concentrations in DnaA overproducing cells, ΔdatA cells and DiaA overproducing cells.
1) ng DnaA per μg cell extract. The numbers are relative to the wild type or wild type containing the empty plasmid. Measured by imunoblotting.± represents the standard deviationDnaA concentrations in DnaA overproducing cells, ΔdatA cells and DiaA ...
Riikka Taipale (750567) +4 more
core +1 more source
In Situ Bacterioplankton Growth Partitioning by High‐Resolution Metatranscriptomics
Bacterial growth and primary production are key ecological indicators, yet often measured in bulk. Using metatranscriptomics, the authors identify FtsZ expression to track dividing cells (A, B). Phylogenetic placement of transcripts (C–E) enables linking growth activity to specific taxa (E, F), potentially bridging bulk measurements with fine‐scale ...
J. Cesar Ignacio‐Espinoza +5 more
wiley +1 more source
Multiplex Modular Nanorobotic Systems with Catalytic Activity under Magnetic Navigation
We present modular nanorobots composed of a magnetic propulsion module and different extension modules carrying functional activities, assembled via DNA base pairing. The system integrates propulsion, optical tracking, enzymatic catalysis, and cell docking in a programmable manner.
Voichita Mihali +7 more
wiley +1 more source
AdpA, key regulator for morphological differentiation regulates bacterial chromosome replication [PDF]
AdpA, one of the most pleiotropic transcription regulators in bacteria, controls expression of several dozen genes during Streptomyces differentiation.
Marcin Wolański +2 more
doaj +1 more source
In vivo dynamics of DnaA and its regulators
Die Verdopplung des Chromosoms ist ein entscheidender Schritt des bakteriellen Zellzyklus. Um die Anzahl der Chromosomen pro Zelle konstant zu halten, muss dieser Prozess streng reguliert werden.
Schenk, Katrin +1 more
core +2 more sources
Structure and Function of the Campylobacter jejuni Chromosome Replication Origin
Campylobacter jejuni is the leading bacterial cause of foodborne infections worldwide. However, our understanding of its cell cycle is poor. We identified the probable C.
Pawel Jaworski +5 more
doaj +1 more source
Discordance Between 16S rRNA Similarity and Genome‐Based Species Boundaries in Gluconobacter
16S rRNA sequences in Gluconobacter show high conservation with overlapping intra‐ and interspecific similarities, limiting species‐level resolution. In contrast, genome‐based ANI reveals clear divergence and distinct lineages, highlighting a fundamental discordance between 16S rRNA sequence‐based classification and genome‐level relationships ...
Shintaro Maeno +3 more
wiley +1 more source

