Results 91 to 100 of about 17,302 (170)

Genetic variants in microRNA machinery genes are associated [corrected] with idiopathic recurrent pregnancy loss risk.

PLoS ONE, 2014
ObjectiveKey molecules involved in microRNA (miRNA) biogenesis, such as DROSHA, XPO5, and DICER, have been identified in trophoblast cells, confirming that the miRNA biogenesis pathway is active in human placenta.
Yong Wook Jung, Young Joo Jeon, HyungChul Rah, Ji Hyang Kim, Ji Eun Shin, Dong Hee Choi, Sun Hee Cha, Nam Keun Kim   +7 more
doaj   +1 more source

fCLIP-seq for transcriptomic footprinting of dsRNA-binding proteins: Lessons from DROSHA

, 2019
CLIP-seq (crosslinking immunoprecipitation and sequencing) is widely used to map the binding sites of a protein of interest on the transcriptome, and generally employs UV to induce the covalent bonds between protein and RNA, which allows stringent ...
Kim, Baekgyu, Kim, V. Narry, Baekgyu Kim, V. Narry Kim   +3 more
core   +2 more sources

PTEN has inhibitory role for RNH1 interaction to the Drosha complex.

, 2013
(A) Physical interaction between RNH1 and the Drosha complex. The plasmid encoding Drosha-FLAG and HA-RNH1 were ectopically expressed in 293T cells. Drosha-FLAG was precipitated with anti-FLAG M2 affinity agarose gels and then and then immunoblotting ...
Youn-Jae Kim (128538), Hee Jin Kwak (338192), Jong Heon Kim (128540), Se-Jeong Park (338187), Eun Young Choi (338189), Heon Yoo (128542), Seung-Hoon Lee (147230), Daesoo Kim (127687), Sol Kim (331410), Byong Chul Yoo (338195), Jong Bae Park (128544)   +10 more
core   +1 more source

Expression, Covariation, and Genetic Regulation of miRNA Biogenesis Genes in Brain Supports their Role in Addiction, Psychiatric Disorders, and Disease

Frontiers in Genetics, 2013
The role of miRNA and miRNA biogenesis genes in the adult brain is just beginning to be explored. In this study we have performed a comprehensive analysis of the expression, genetic regulation, and co-expression of major components of the miRNA ...
Megan Kathleen Mulligan, Candice eDuBose, Junming eYue, Michael F. Miles, Lu eLu, Lu eLu, Kristin M Hamre   +6 more
doaj   +1 more source

Racial Differences in Expression Levels of miRNA Machinery-Related Genes, Dicer, Drosha, DGCR8, and AGO2, in Asian Korean Papillary Thyroid Carcinoma and Comparative Validation Using the Cancer Genome Atlas

International Journal of Genomics, 2017
Aberrant regulation of microRNA (miRNA) machinery components is associated with various human cancers, including papillary thyroid carcinoma (PTC), which is the most common type of thyroid cancer, and a higher prevalent female malignancy.
Jaegil Kim, Woo-Jae Park, Kwang-Joon Jeong, Sun Hee Kang, Sun Young Kwon, Shin Kim, Jong-Wook Park   +6 more
doaj   +1 more source

Interdependence of Pasha and Drosha for localization and function of the Microprocessor in C. elegans

Nature Communications
Primary microRNA (pri-miRNA) transcripts are processed by the Microprocessor, containing the ribonuclease Drosha and its RNA-binding partner DGCR8/Pasha. In a forward genetic screen utilizing a fluorescence-based sensor that monitors pri-miRNA processing
Thiago L. Knittel, Brooke E. Montgomery, Kailee J. Reed, Madeleine C. Chong, Ida J. Isolehto, Erin R. Cafferty, Margaret J. Smith, Reese A. Sprister, Colin N. Magelky, Hataichanok Scherman, René F. Ketting, Taiowa A. Montgomery   +11 more
doaj   +1 more source

Flow chart for the method of Drosha processing site prediction.

, 2012
Flow chart for the method of Drosha processing site prediction.
Chenfeng He (107020), Zuguang Gu (107027), Guangxin Zhang (107025), Zhi John Lu (107029), Zhi-Hua Zhou (107030), Rong Yang (107028), Ying-Xin Li (107023), Jie Li (15030), Jin Wang (29560), Chenyu Zhang (107032)   +9 more
core   +1 more source

DROSHA compromised HCT116 cells are resistant to ER stress-induced cell death.

, 2013
A) QRT-PCR for Drosha in HCT116 WT, and DROSHA shRNA cells, with or without 500 ng/ml of Doxycycline. B) QRT-PCR for Pri-miRNA-17 WT and DROSHA shRNA cells with Doxycycline for three days. C) QRT-PCR for miRNA in WT and DROSHA shRNA cells with or without
Susan E. Logue (447410), Adrienne M. Gorman (447411), Afshin Samali (161678), Qingping Zeng (447412), Sanjeev Gupta (161674), John Patterson (447413), Karen Cawley (447409)   +6 more
core   +1 more source

In vitro Drosha processing of miRNA Rolling Circle Transcripts (RCT).

, 2013
A. Deproteinized miR-19am RCTs made in vitro using E. coli RNA polymerase were incubated with HEK293T whole cell extract (WCE, Lane 2); with the Flag-Drosha complex immunoprecipitated from Flag-Drosha-expressing HEK293T WCE (Lane 4); or with Flag ...
Kevin Ryan (356118), Christine I. Seidl (356117)   +1 more
core   +1 more source

Non-canonical RNA substrates of Drosha lack many of the conserved features found in primary microRNA stem-loops

Scientific Reports
The RNase III enzyme Drosha has a central role in microRNA (miRNA) biogenesis, where it is required to release the stem-loop intermediate from primary (pri)-miRNA transcripts. However, it can also cleave stem-loops embedded within messenger (m)RNAs. This
Karen Gu, Lawrence Mok, Matthew J. Wakefield, Mark M. W. Chong   +3 more
doaj   +1 more source