Results 11 to 20 of about 16,723 (215)

Structure of Human DROSHA [PDF]

Cell, 2016
MicroRNA maturation is initiated by RNase III DROSHA that cleaves the stem loop of primary microRNA. DROSHA functions together with its cofactor DGCR8 in a heterotrimeric complex known as Microprocessor. Here, we report the X-ray structure of DROSHA in complex with the C-terminal helix of DGCR8. We find that DROSHA contains two DGCR8-binding sites, one
Kwon, S. Chul, Nguyen, Tuan Anh, Choi, Yeongil, Jo, Myunghyun, Hohng, Sungchul C., Kim, V. Narry, Woo, Jae-Sung   +6 more
core   +5 more sources

Drosha promotes splicing of a pre-microRNA-like alternative exon.

PLoS Genetics, 2014
The ribonuclease III enzyme Drosha has a central role in the biogenesis of microRNA (miRNA) by binding and cleaving hairpin structures in primary RNA transcripts into precursor miRNAs (pre-miRNAs).
Mallory A Havens, Ashley A Reich, Michelle L Hastings   +2 more
doaj   +2 more sources

The Drosha-DGCR8 complex in primary microRNA processing [PDF]

Genes & Development, 2004
RNase III proteins play key roles in microRNA (miRNA) biogenesis. The nuclear RNase III Drosha cleaves primary miRNAs (pri-miRNAs) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic RNase III Dicer to generate mature miRNAs.
Jinju, Han, Yoontae, Lee, Kyu-Hyun, Yeom, Young-Kook, Kim, Hua, Jin, V Narry, Kim   +5 more
openaire   +3 more sources

Dicer, Drosha, and Outcomes in Patients with Ovarian Cancer [PDF]

New England Journal of Medicine, 2008
We studied Dicer and Drosha, components of the RNA-interference machinery, in ovarian cancer.We measured messenger RNA (mRNA) levels of Dicer and Drosha in specimens of invasive epithelial ovarian cancer from 111 patients, using a quantitative reverse-transcriptase-polymerase-chain-reaction assay, and compared the results with clinical outcomes ...
Merritt, W.M., Lin, Y.G., Han, L.Y., Karnat, Aa., Spannuth, W.A., Schmandt, R., Urbauer, D., Pennacchio, L.A., Cheng, J.-F., Nick, A.M., Deavers, M.T., Mourad-Seidan, A., Wang, H., Mueller, P., Lenburg, M.E., Gray, J.W., Mok, S., Birrer, M.J., Lopez-Berestein, G., Coleman, R.L., Bar-Eli, M., Sood, A.K.   +21 more
openaire   +5 more sources

Drosha Regulates Gene Expression Independently of RNA Cleavage Function [PDF]

Cell Reports, 2013
Drosha is the main RNase III-like enzyme involved in the process of microRNA (miRNA) biogenesis in the nucleus. Using whole-genome ChIP-on-chip analysis, we demonstrate that, in addition to miRNA sequences, Drosha specifically binds promoter-proximal ...
Natalia Gromak, Martin Dienstbier, Sara Macias, Mireya Plass, Eduardo Eyras, Javier F. Cáceres, Nicholas J. Proudfoot   +6 more
doaj   +2 more sources

Alternative splicing affects the subcellular localization of Drosha [PDF]

Nucleic Acids Research, 2016
The RNase III enzyme Drosha is a key factor in microRNA (miRNA) biogenesis and as such indispensable for cellular homeostasis and developmental processes. Together with its co-factor DGCR8, it converts the primary transcript (pri-miRNA) into the precursor hairpin (pre-miRNA) in the nucleus. While the middle and the C-terminal domain are crucial for pri-
Link, Steffen, Grund, Stefanie E., Diederichs, Sven   +2 more
openaire   +4 more sources

Deregulation of Drosha in the pathogenesis of hereditary hemorrhagic telangiectasia [PDF]

Current Opinion in Hematology, 2019
Purpose of review The TGFβ (transforming growth factor β) superfamily – a large group of structurally related and evolutionarily conserved proteins – profoundly shapes and organizes the vasculature during normal development and adult homeostasis.
Hata, Akiko, Lagna, Giorgio
openaire   +5 more sources

Conservation of Differential Animal MicroRNA Processing by Drosha and Dicer [PDF]

Frontiers in Molecular Biosciences, 2022
In complex biochemical systems, an enzyme, protein, or RNA, symbolized as E, has hundreds or thousands of substrates or interacting partners. The relative specificity hypothesis proposes that such an E would differentially interact with and influence its
Xiaoxiao Zhang, Fanming Yang, Fanzou Liu, Qiuhuan Tian, Min Hu, Peng Li, Yan Zeng   +6 more
doaj   +3 more sources

The Drosha rs10719 T>C polymorphism is associated with preeclampsia susceptibility

Clinical and Experimental Hypertension, 2018
Purpose: Drosha is a member of the micro RNA (miRNA) processing machinery that affects miRNA processing. Single-nucleotide polymorphisms (SNPs) in the Drosha gene might affect microRNA processing and the expression of various genes. The aim of this study
Mahnaz Rezaei, Fatemeh Eskandari, Abbas Mohammadpour-Gharehbagh, Batool Teimoori, Minoo Yaghmaei, Mojgan Mokhtari, Saeedeh Salimi   +6 more
doaj   +2 more sources

Drosha in Primary MicroRNA Processing [PDF]

Cold Spring Harbor Symposia on Quantitative Biology, 2006
MicroRNA (miRNA)-mediated gene silencing is one of the major regulatory pathways in eukaryotes. Much effort has been made to identify the factors involved in the pathway, and our understanding of RNA silencing has significantly advanced in recent years.
Y, Lee, J, Han, K-H, Yeom, H, Jin, V N, Kim   +4 more
openaire   +2 more sources