Formation of Spindle Poles by Dynein/Dynactin-Dependent Transport of Numa [PDF]
, 2000 NuMA is a large nuclear protein whose relocation to the spindle poles is required for bipolar mitotic spindle assembly. We show here that this process depends on directed NuMA transport toward microtubule minus ends powered by cytoplasmic dynein and its ...
William C. Earnshaw +9 more
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Cytoplasmic dynein and its regulatory proteins in Golgi pathology in nervous system disorders
Frontiers in Neuroscience, 2015 The Golgi apparatus is a dynamic organelle involved in processing and sorting of lipids and proteins. In neurons, the Golgi apparatus is important for the development of axons and dendrites and maintenance of their highly polarized morphology.
Dick eJaarsma, Casper eHoogenraad
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A PIK3C3–Ankyrin-B–Dynactin pathway promotes axonal growth and multiorganelle transport [PDF]
, 2014 Axon growth requires long-range transport of organelles, but how these cargoes recruit their motors and how their traffic is regulated are not fully resolved. In this paper, we identify a new pathway based on the class III PI3-kinase (PIK3C3), ankyrin-B (
Zhong, G. +15 more
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RNA-directed activation of cytoplasmic dynein-1 in reconstituted transport RNPs
eLife, 2018 Polarised mRNA transport is a prevalent mechanism for spatial control of protein synthesis. However, the composition of transported ribonucleoprotein particles (RNPs) and the regulation of their movement are poorly understood.
Mark A McClintock +6 more
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Dynein–Dynactin–NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble
eLife, 2018 To position the mitotic spindle within the cell, dynamic plus ends of astral microtubules are pulled by membrane-associated cortical force-generating machinery.
Masako Okumura +3 more
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Molecular characterization of the 50-kD subunit of dynactin reveals function for the complex in chromosome alignment and spindle organization during mitosis [PDF]
, 2008 Dynactin is a multi-subunit complex which has been implicated in cytoplasmic dynein function, though its mechanism of action is unknown. In this study, we have characterized the 50-kD subunit of dynactin, and analyzed the effects of its overexpression on
Echeverri, Christophe de Jesus +3 more
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Differential Effects of LIS1 On Processive Dynein-Dynactin Motility
, 2017 Cytoplasmic dynein is the primary minus-end directed microtubule motor protein in cells. LIS1 is a highly conserved dynein regulatory factor that binds directly to the dynein motor domain, uncoupling the enzymatic and mechanical cycles of the motor, and ...
Richard J. McKenney, Pedro A. Gutierrez
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, 2019 Bidirectional cargo transport in neurons can be explained by two models: the "tug-of-war model" for short-range transport, in which several kinesin and dynein motors are bound to the same cargo but travel in opposing directions, and by the "motor ...
Ying‐Cheng Yen +13 more
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Nature Communications, 2019 LGN and NuMA link epithelial polarity sites with astral microtubules and associate with dynein, but the architecture of such cortical force-generating complexes is unknown.
Laura Pirovano +10 more
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The interaction between cytoplasmic dynein and dynactin in intracellular transport
, 1997 Coordinated movement of organelles along the polarized array of microtubules is critical in diverse cellular processes such as mitosis, exocytosis, and axonal transport.
Karki, Sher Bahadur
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