Results 91 to 100 of about 4,333 (201)

Engineered Tissue Models to Decode Host–Microbiota Interactions

Advanced Science, Volume 12, Issue 23, June 20, 2025.
Host–Microbiota interactions in the human body. Created in BioRender. Ghezzi, C. (2025) https://BioRender.com/ihivskg. Abstract A mutualistic co‐evolution exists between the host and its associated microbiota in the human body. Bacteria establish ecological niches in various tissues of the body, locally influencing their physiology and functions, but ...
Miryam Adelfio, Grace E. Callen, Xuesong He, Bruce J. Paster, Hatice Hasturk, Chiara E. Ghezzi   +5 more
wiley   +1 more source

Equine coronavirus infection and replication in equine intestinal enteroids. [PDF]

Vet Res
Abstract In this study, equine intestinal enteroids (EIEs) were generated from the duodenum, jejunum, and ileum and inoculated with equine coronavirus (ECoV) to investigate their suitability as in vitro models with which to study ECoV infection. Immunohistochemistry revealed that the EIEs were composed of various cell types expressed in vivo in
Kambayashi Y, Nemoto M, Ochi A, Kishi D, Ueno T, Tsujimura K, Bannai H, Kawanishi N, Ohta M, Suzuki T.   +9 more
europepmc   +4 more sources

GUCY2C maintains intestinal LGR5+ stem cells by opposing ER stress [PDF]

, 2017
Long-lived multipotent stem cells (ISCs) at the base of intestinal crypts adjust their phenotypes to accommodate normal maintenance and post-injury regeneration of the epithelium.
Kraft, Crystal, Lynch, John P., Pattison, Amanda M., Rappaport, Jeff A., Snook, Adam E., Waldman, Scott A.   +5 more
core   +2 more sources

A Dynamic Double‐Flow Gut‐On‐Chip Model for Predictive Absorption Studies In Vitro

Advanced Materials Technologies, Volume 10, Issue 9, May 6, 2025.
This study presents a novel millifluidic gut‐on‐chip model using the MIVO® device to replicate human intestinal conditions. Dynamic co‐culture of CaCo‐2 and HT‐29 cells accelerates differentiation, modulates mucus thickness, and enhances barrier function.
Maria Elisabetta Federica Palamà, Maurizio Aiello, Gergő Borka, Jacopo Furci, Ilaria Parodi, Giuseppe Firpo, Silvia Scaglione   +6 more
wiley   +1 more source

Identification of Reg3β-producing cells using IL-22-stimulated enteroids

Bioscience, Biotechnology, and Biochemistry, 2020
ABSTRACT Reg3β, a lectin, displays antibacterial activity. This study investigated Reg3β-expressing cells using IL-22-stimulated enteroids. IL-22 stimulation elevated the mRNA and protein levels of Reg3β. IL-22 also increased the mRNA levels of CD133 (a transit-amplifying cell marker) and lysozyme (a Paneth cell marker ...
Mika, Sato, Akihiko, Inaba, Ken, Iwatsuki, Yuki, Saito, Miki, Tadaishi, Makoto, Shimizu, Kazuo, Kobayashi-Hattori   +6 more
openaire   +2 more sources

Replication of human noroviruses in stem cell–derived human enteroids [PDF]

Science, 2016
The major barrier to research and development of effective interventions for human noroviruses (HuNoVs) has been the lack of a robust and reproducible in vitro cultivation system. HuNoVs are the leading cause of gastroenteritis worldwide. We report the successful cultivation of multiple HuNoV strains in enterocytes in stem cell–derived, nontransformed ...
Khalil, Ettayebi, Sue E, Crawford, Kosuke, Murakami, James R, Broughman, Umesh, Karandikar, Victoria R, Tenge, Frederick H, Neill, Sarah E, Blutt, Xi-Lei, Zeng, Lin, Qu, Baijun, Kou, Antone R, Opekun, Douglas, Burrin, David Y, Graham, Sasirekha, Ramani, Robert L, Atmar, Mary K, Estes   +16 more
openaire   +2 more sources

Production and characterization of avian crypt-villus enteroids and the effect of chemicals

BMC Veterinary Research, 2020
Background Three-dimensional models of cell culture such as organoids and mini organs accord better advantage over regular cell culture because of their ability to simulate organ functions hence, used for disease modeling, metabolic research, and the ...
Mohan Acharya, Komala Arsi, Annie M. Donoghue, Rohana Liyanage, Narayan C. Rath   +4 more
doaj   +1 more source

Reduced lysosomal acid lipase activity: A new marker of liver disease severity across the clinical continuum of non-alcoholic fatty liver disease? [PDF]

, 2019
Lysosomal acid lipase (LAL) plays a key role in intracellular lipid metabolism. Reduced LAL activity promotes increased multi-organ lysosomal cholesterol ester storage, as observed in two recessive autosomal genetic diseases, Wolman disease and ...
Angelico, F, Baratta, F, Carluccio, G, Del Ben, M, Ferro, D, Pastori, D, Tozzi, G, Violi, F   +7 more
core   +1 more source

Hemagglutinin Protease HapA Associated With Vibrio cholerae Outer Membrane Vesicles (OMVs) Disrupts Tight and Adherens Junctions

Journal of Extracellular Vesicles, Volume 14, Issue 5, May 2025.
ABSTRACT This study explores the virulence mechanisms of Vibrio cholerae, with a particular emphasis on HapA, a zinc metalloprotease delivered via outer membrane vesicles (OMVs). The findings reveal that OMV‐associated HapA disrupts the integrity of tight and adherens junctions in intestinal epithelial cell models more effectively than its purified ...
Palwasha Baryalai, David Irenaeus, Eric Toh, Madeleine Ramstedt, Bernt Eric Uhlin, Aftab Nadeem, Sun Nyunt Wai   +6 more
wiley   +1 more source

Insights into human norovirus cultivation in human intestinal enteroids

mSphere
ABSTRACT Human noroviruses (HuNoVs) are a significant cause of epidemic and sporadic acute gastroenteritis worldwide. The lack of a reproducible culture system hindered the study of HuNoV replication and pathogenesis for almost a half-century.
Khalil Ettayebi, Gurpreet Kaur, Ketki Patil, Janam Dave, B. Vijayalakshmi Ayyar, Victoria R Tenge, Frederick H. Neill, Xi-Lei Zeng, Allison L. Speer, Sara C. Di Rienzi, Robert A. Britton, Sarah E. Blutt, Sue E. Crawford, Sasirekha Ramani, Robert L. Atmar, Mary K. Estes   +15 more
openaire   +5 more sources