Results 71 to 80 of about 3,117 (204)

Image_3_Human Intestinal Enteroids Model MHC-II in the Gut Epithelium.JPEG

, 2019
The role of intestinal epithelial cells (IECs) in mucosal tolerance and immunity remains poorly understood. We present a method for inducing MHC class II (MHC-II) in human enteroids, “mini-guts” derived from small intestinal crypt stem cells, and show ...
Jonathan E. Wosen (7280258), Manuel R. Amieva (7280267), Nielsen Q. Fernandez-Becker (6001403), Elizabeth D. Mellins (7280270), Calvin J. Kuo (7280264), Julia Y. Co (5964173), Alexandra Ilstad-Minnihan (4385836), Wei Jiang (14986), Dhriti Mukhopadhyay (7280261)   +8 more
core   +1 more source

Distinct Roles of SLC26A3 and CFTR in Surface pH Regulation and Bicarbonate Secretion in Human Intestinal Epithelium

Acta Physiologica, Volume 242, Issue 2, February 2026.
ABSTRACT Background and Aims Colonic bicarbonate secretion is mediated by the chloride/bicarbonate exchanger SLC26A3 and the cystic fibrosis transmembrane conductance regulator (CFTR). Dysfunction of either causes luminal acidosis, altered mucus properties, and inflammation.
Mahdi Amiri, Azam Salari, Ursula Seidler
wiley   +1 more source

Type 1 diabetes human enteroid studies reveal major changes in the intestinal epithelial compartment

Scientific Reports
Lack of understanding of the pathophysiology of gastrointestinal (GI) complications in type 1 diabetes (T1D), including altered intestinal transcriptomes and protein expression represents a major gap in the management of these patients.
Vishwesh Bharadiya, Yan Rong, Zixin Zhang, Ruxian Lin, Anthony Lawrence Guerrerio, C. Ming Tse, Mark Donowitz, Varsha Singh   +7 more
doaj   +1 more source

Quantitative Assessment of Gut and Vascular Barriers in TTOP, a Cartridge‐Based Bicompartmental Culture Platform

Advanced Materials Technologies, Volume 11, Issue 1, 8 January 2026.
The modular, versatile, and standardized cartridge‐based culture module allows to replicate gut and vascular barriers. Its conventional handling requirements facilitate adoption by non‐technical users, while the design allows safe manipulation and retrieval of samples for permeability assays and endpoint analysis.
Lorenzo Pietro Coppadoro, Maria Lombardi, Sabrina Nicolò, Alessandra Maria Anna Rando, Gianfranco Beniamino Fiore, Chiara Foglieni, Monica Soncini   +6 more
wiley   +1 more source

Image_7_Human Intestinal Enteroids Model MHC-II in the Gut Epithelium.JPEG

, 2019
The role of intestinal epithelial cells (IECs) in mucosal tolerance and immunity remains poorly understood. We present a method for inducing MHC class II (MHC-II) in human enteroids, “mini-guts” derived from small intestinal crypt stem cells, and show ...
Jonathan E. Wosen (7280258), Manuel R. Amieva (7280267), Nielsen Q. Fernandez-Becker (6001403), Elizabeth D. Mellins (7280270), Calvin J. Kuo (7280264), Julia Y. Co (5964173), Alexandra Ilstad-Minnihan (4385836), Wei Jiang (14986), Dhriti Mukhopadhyay (7280261)   +8 more
core   +1 more source

Apical-out chicken enteroids with leukocyte component as a model to study host-pathogen interactions [PDF]

, 2022
Over the last 20 years global poultry production has tripled with approximately 107 million tonnes of chicken meat and 1.3 trillion eggs now produced every year (FAOSTAT, 2019).
Nash, Tessa
core   +1 more source

Decoding Host–Pathogen Interactions Through Caenorhabditis elegans: A Narrative Case Study

Advanced Gut &Microbiome Research, Volume 2026, Issue 1, 2026.
Infectious diseases continue to pose a significant challenge to global health, particularly with the emergence of multidrug‐resistant pathogens. Understanding host–pathogen interactions (HPIs) is fundamental to uncovering mechanisms of infection, immunity, and adaptation.
Balasubramanian Chellammal Muthubharathi, Krishnaswamy Balamurugan, Jian Wu   +2 more
wiley   +1 more source

Protocol for the cryopreservation of Cryptosporidium isolates using enteroids

STAR Protocols
A major bottleneck in the progress of Cryptosporidium research is the lack of accessible cryopreservation of Cryptosporidium oocysts. Here, we present a protocol for the cryopreservation of Cryptosporidium isolates using enteroids. We describe the steps for the establishment of enteroid cultures and cryopreservation of C. parvum-infected HCT-8 cultures.
Miner Deng, Yanting Wei, Na Li, Lihua Xiao, Yaoyu Feng, Yaqiong Guo   +5 more
openaire   +3 more sources

Image_9_Human Intestinal Enteroids Model MHC-II in the Gut Epithelium.JPEG

, 2019
The role of intestinal epithelial cells (IECs) in mucosal tolerance and immunity remains poorly understood. We present a method for inducing MHC class II (MHC-II) in human enteroids, “mini-guts” derived from small intestinal crypt stem cells, and show ...
Jonathan E. Wosen (7280258), Manuel R. Amieva (7280267), Nielsen Q. Fernandez-Becker (6001403), Elizabeth D. Mellins (7280270), Calvin J. Kuo (7280264), Julia Y. Co (5964173), Alexandra Ilstad-Minnihan (4385836), Wei Jiang (14986), Dhriti Mukhopadhyay (7280261)   +8 more
core   +1 more source

Additional file 7 of Temporal transcriptome profiling of floating apical out chicken enteroids suggest stability and reproducibility

, 2023
Additional file 7. Proliferation in the enteroids. Graph demonstrating the percentage of proliferating (EdU+) cells within enteroids over 7 days of culture, alongside z-stack image of proliferating (EdU+) cells within a 14 h ...
Katrina M. Morris (3247860), Lonneke Vervelde (15229027), Tessa J. Nash (15229024), Neil A. Mabbott (7361273)   +3 more
core   +1 more source