Results 21 to 30 of about 10,382 (181)

Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission

eLife, 2022
The 12 related human ESCRT-III proteins form filaments that constrict membranes and mediate fission, including during cytokinetic abscission. The C-terminal tails of polymerized ESCRT-III subunits also bind proteins that contain Microtubule-Interacting ...
Dawn M Wenzel, Douglas R Mackay, Jack J Skalicky, Elliott L Paine, Matthew S Miller, Katharine S Ullman, Wesley I Sundquist   +6 more
doaj   +1 more source

Novel Interactions of ESCRT-III with LIP5 and VPS4 and their Implications for ESCRT-III Disassembly [PDF]

Molecular Biology of the Cell, 2008
The AAA+ ATPase VPS4 plays an essential role in multivesicular body biogenesis and is thought to act by disassembling ESCRT-III complexes. VPS4 oligomerization and ATPase activity are promoted by binding to LIP5. LIP5 also binds to the ESCRT-III like protein CHMP5/hVps60, but how this affects its function remains unclear.
Shim, Soomin, Merrill, Samuel A, Hanson, Phyllis I   +2 more
openaire   +3 more sources

CC2D1A Is a Regulator of ESCRT-III CHMP4B [PDF]

Journal of Molecular Biology, 2012
Endosomal sorting complexes required for transport (ESCRTs) regulate diverse processes ranging from receptor sorting at endosomes to distinct steps in cell division and budding of some enveloped viruses. Common to all processes is the membrane recruitment of ESCRT-III that leads to membrane fission.
Martinelli, Nicolas, Hartlieb, Bettina, Usami, Yoshiko, Sabin, Charles, Dordor, Aurelien, Miguet, Nolwenn, Avilov, Sergiy, Ribeiro, Euripedes A, Göttlinger, Heinrich, Weissenhorn, Winfried   +9 more
openaire   +3 more sources

Structural Basis for Autoinhibition of ESCRT-III CHMP3 [PDF]

Journal of Molecular Biology, 2008
Endosomal sorting complexes required for transport (ESCRT-0, ESCRT-I, ESCRT-II, and ESCRT-III) are selectively recruited to cellular membranes to exert their function in diverse processes, such as multivesicular body biogenesis, enveloped virus budding, and cytokinesis.
Lata, S, Roessle, M, Solomons, J, Jamin, M, Göttlinger, H.G, Svergun, D.I, Weissenhorn, W   +6 more
openaire   +4 more sources

Helical Structures of ESCRT-III Are Disassembled by VPS4 [PDF]

Science, 2008
During intracellular membrane trafficking and remodeling, protein complexes known as the ESCRTs (endosomal sorting complexes required for transport) interact with membranes and are required for budding processes directed away from the cytosol, including the budding of intralumenal vesicles to form multivesicular bodies; for the budding of some ...
Suman, Lata, Guy, Schoehn, Ankur, Jain, Ricardo, Pires, Jacob, Piehler, Heinrich G, Gottlinger, Winfried, Weissenhorn   +6 more
openaire   +3 more sources

Functional Reconstitution of ESCRT-III Assembly and Disassembly [PDF]

Cell, 2009
Receptor downregulation in the MVB pathway is mediated by the ESCRT complexes. ESCRT-III is composed of four protein subunits that are monomeric in the cytosol and oligomerize into a protein lattice only upon membrane binding. Recent studies have shown that the ESCRT-III protein Snf7 can form a filament by undergoing homo-oligomerization.
Saksena, Suraj, Wahlman, Judit, Teis, David, Johnson, Arthur E., Emr, Scott D.   +4 more
openaire   +6 more sources

Physical mechanisms of ESCRT-III-driven cell division. [PDF]

Proc Natl Acad Sci U S A, 2022
Significance Cell division is an essential requirement for life. Division requires mechanical forces, often exerted by protein assemblies from the cell interior, that split a single cell into two. Using coarse-grained computer simulations and live cell imaging we define a distinct cell division mechanism—based on ...
Harker-Kirschneck L, Hafner AE, Yao T, Vanhille-Campos C, Jiang X, Pulschen A, Hurtig F, Hryniuk D, Culley S, Henriques R, Baum B, Šarić A.   +11 more
europepmc   +5 more sources

The ESCRT Machinery: Remodeling, Repairing, and Sealing Membranes

Membranes, 2022
The ESCRT machinery is an evolutionarily conserved membrane remodeling complex that is used by the cell to perform reverse membrane scission in essential processes like protein degradation, cell division, and release of enveloped retroviruses. ESCRT-III,
Yolanda Olmos
doaj   +1 more source

Structural basis for ESCRT-III protein autoinhibition [PDF]

Nature Structural & Molecular Biology, 2009
Endosomal sorting complexes required for transport-III (ESCRT-III) subunits cycle between two states: soluble monomers and higher-order assemblies that bind and remodel membranes during endosomal vesicle formation, midbody abscission and enveloped virus budding.
Bajorek, Monika, Schubert, Heidi L., McCullough, John, Langelier, Charles, Eckert, Debra M., Stubblefield, William-May B., Uter, Nathan T., Myszka, David G., Hill, Christopher P., Sundquist, Wesley I.   +9 more
openaire   +2 more sources

AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody.

PLoS Genetics, 2021
To complete mitosis, the bridge that links the two daughter cells needs to be cleaved. This step is carried out by the endosomal sorting complex required for transport (ESCRT) machinery. AKTIP, a protein discovered to be associated with telomeres and the
Chiara Merigliano, Romina Burla, Mattia La Torre, Simona Del Giudice, Hsiangling Teo, Chong Wai Liew, Alexandre Chojnowski, Wah Ing Goh, Yolanda Olmos, Klizia Maccaroni, Maria Giubettini, Irene Chiolo, Jeremy G Carlton, Domenico Raimondo, Fiammetta Vernì, Colin L Stewart, Daniela Rhodes, Graham D Wright, Brian E Burke, Isabella Saggio   +19 more
doaj   +1 more source