Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM
Cell Reports, 2018 Summary: The ESCRT machinery mediates membrane fission in a variety of processes in cells. According to current models, ESCRT-III proteins drive membrane fission by assembling into helical filaments on membranes.
Inna Goliand +6 more
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Novel Interactions of ESCRT-III with LIP5 and VPS4 and their Implications for ESCRT-III Disassembly [PDF]
Molecular Biology of the Cell, 2008 The AAA+ ATPase VPS4 plays an essential role in multivesicular body biogenesis and is thought to act by disassembling ESCRT-III complexes. VPS4 oligomerization and ATPase activity are promoted by binding to LIP5. LIP5 also binds to the ESCRT-III like protein CHMP5/hVps60, but how this affects its function remains unclear.
Shim, Soomin +2 more
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ESCRT-III activation by parallel action of ESCRT-I/II and ESCRT-0/Bro1 during MVB biogenesis
eLife, 2016 The endosomal sorting complexes required for transport (ESCRT) pathway facilitates multiple fundamental membrane remodeling events. Previously, we determined X-ray crystal structures of ESCRT-III subunit Snf7, the yeast CHMP4 ortholog, in its active and ...
Shaogeng Tang +5 more
doaj +1 more source
Animal cell cytokinesis: the role of dynamic changes in the plasma membrane proteome and lipidome [PDF]
, 2016 In animal cells, cytokinesis is characterised by the formation of the mitotic spindle that signals the assembly of an actomyosin ring between the spindle poles.
Gould, Gwyn W.
core +2 more sources
ESCRT-III/Vps4 controls heterochromatin-nuclear envelope attachments [PDF]
Developmental Cell, 2019 AbstractIn eukaryotes chromosomes are compartmentalized within the nucleus delimited by the double membrane of the nuclear envelope (NE). Defects in the function and structure of the NE are linked to disease1,2. During interphase, the NE organizes the genome and regulates its expression3.
Pieper, Gerard H. +3 more
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Principles of membrane remodeling by dynamic ESCRT-III polymers [PDF]
Trends in Cell Biology, 2021 Endosomal protein complex required for transport-III (ESCRT-III) polymers are involved in many crucial cellular functions, from cell division to endosome-lysosome dynamics. As a eukaryotic membrane remodeling machinery, ESCRT-III is unique in its ability to catalyze fission of membrane necks from their luminal side and to participate in membrane ...
Pfitzner, Anna-Katharina +2 more
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SPG20 protein spartin is recruited to midbodies by ESCRT-III protein Ist1 and participates in cytokinesis. [PDF]
, 2010 Hereditary spastic paraplegias (HSPs, SPG1-46) are inherited neurological disorders characterized by lower extremity spastic weakness. Loss-of-function SPG20 gene mutations cause an autosomal recessive HSP known as Troyer syndrome.
Bakowska, Joanna C +5 more
core +3 more sources
Structural Basis for Autoinhibition of ESCRT-III CHMP3 [PDF]
Journal of Molecular Biology, 2008 Endosomal sorting complexes required for transport (ESCRT-0, ESCRT-I, ESCRT-II, and ESCRT-III) are selectively recruited to cellular membranes to exert their function in diverse processes, such as multivesicular body biogenesis, enveloped virus budding, and cytokinesis.
Lata, S +6 more
openaire +4 more sources
Membrane Remodeling: ESCRT-III Filaments as Molecular Garrotes [PDF]
Current Biology, 2020 New reconstitution, biochemical and structural studies are revealing how the core machinery of the ESCRT pathway constricts membranes to promote fission. Equally exciting is the discovery and characterization of conserved ESCRT-like machinery across all three domains of life.
John, McCullough, Wesley I, Sundquist
openaire +2 more sources
CDK1 controls CHMP7-dependent nuclear envelope reformation
eLife, 2021 Through membrane sealing and disassembly of spindle microtubules, the Endosomal Sorting Complex Required for Transport-III (ESCRT-III) machinery has emerged as a key player in the regeneration of a sealed nuclear envelope (NE) during mitotic exit, and in
Alberto T Gatta +5 more
doaj +1 more source