Results 51 to 60 of about 10,382 (181)

Translating whole‐genome doubling into precision medicine in cancer

Molecular Oncology, EarlyView.
Whole‐genome doubling creates a WGD‐positive tumor state characterized by persistent chromosomal instability, karyotypic diversification, and cellular stress. These same biological pressures drive aggressive tumor evolution while exposing therapeutic vulnerabilities, providing a rationale for WGD‐informed precision medicine. Whole‐genome doubling (WGD)
Sejung Lee, Junghyeok Lim, Jinhyuk Bhin
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Structural Basis for Budding by the ESCRT-III Factor CHMP3 [PDF]

Developmental Cell, 2006
The vacuolar protein sorting machinery regulates multivesicular body biogenesis and is selectively recruited by enveloped viruses to support budding. Here we report the crystal structure of the human ESCRT-III protein CHMP3 at 2.8 A resolution. The core structure of CHMP3 folds into a flat helical arrangement that assembles into a lattice, mainly via ...
Muzioł, Tadeusz, Pineda-Molina, Estela, Ravelli, Raimond B., Zamborlini, Alessia, Usami, Yoshiko, Göttlinger, Heinrich, Weissenhorn, Winfried   +6 more
openaire   +2 more sources

ESCRT Requirements for Murine Leukemia Virus Release

Viruses, 2016
The Murine Leukemia Virus (MLV) is a gammaretrovirus that hijack host components of the endosomal sorting complex required for transport (ESCRT) for budding.
Christina Bartusch, Reinhild Prange
doaj   +1 more source

Dynamic Mechanical Loading Modulates Extracellular Vesicle Production and Cargo in Meniscus Fibrochondrocytes

Advanced Materials Interfaces, EarlyView.
Dynamic mechanical loading of outer meniscus fibrochondrocytes increases extracellular vesicle (EV) yield and alters EV composition. When applied to progenitor cells, these mechanically primed EVs modulate the expression of matrix‐related genes.
Catherine Cheung, Zizhao Li, Sung‐Han Jo, Se‐Hwan Lee, Il Keun Kwon, Jina Ko, Su Chin Heo   +6 more
wiley   +1 more source

Membrane Remodeling: ESCRT-III Filaments as Molecular Garrotes [PDF]

Current Biology, 2020
New reconstitution, biochemical and structural studies are revealing how the core machinery of the ESCRT pathway constricts membranes to promote fission. Equally exciting is the discovery and characterization of conserved ESCRT-like machinery across all three domains of life.
John, McCullough, Wesley I, Sundquist
openaire   +2 more sources

Structural basis for activation, assembly and membrane binding of ESCRT-III Snf7 filaments

eLife, 2015
The endosomal sorting complexes required for transport (ESCRTs) constitute hetero-oligomeric machines that catalyze multiple topologically similar membrane-remodeling processes.
Shaogeng Tang, W Mike Henne, Peter P Borbat, Nicholas J Buchkovich, Jack H Freed, Yuxin Mao, J Christopher Fromme, Scott D Emr   +7 more
doaj   +1 more source

High‐Throughput Mechanical Rupture of Nuclear Envelope and the Intracellular Dynamics of Massive Wound Repair

Advanced Science, EarlyView.
A microfluidic device with monolithically integrated nanostructures enables high‐throughput wounding of the cell membrane and nuclear envelope. The spatial distribution of charged multivesicular body protein 4B (CHMP4B) within the cell is mapped before and after wounding, showing involvement in repair of both the cell membrane and nuclear envelope ...
Apresio K. Fajrial, Leyla Akh, Stephanie E. Schneider, Wei Tan, Corey P. Neu, Xiaoyun Ding   +5 more
wiley   +1 more source

CK2 involvement in ESCRT-III complex phosphorylation

Archives of Biochemistry and Biophysics, 2014
The multivesicular body (MVB) sorting pathway is a mechanism for delivering transmembrane proteins into the lumen of the lysosome for degradation. ESCRT-III is the final complex in the pathway that assembles on endosomes and executes membrane scission of intraluminal vesicles.
SALVI, MAURO, Camilla Raiborg, Phyllis I. Hanson, Coen Campsteijn, Harald Stenmark, Lorenzo A. Pinna   +5 more
openaire   +3 more sources

ESCRT-III Protein Requirements for HIV-1 Budding [PDF]

Cell Host & Microbe, 2011
Two early-acting components of the cellular ESCRT pathway, ESCRT-I and ALIX, participate directly in HIV-1 budding. The membrane fission activities of ESCRT-III subunits are also presumably required, but humans express 11 different CHMP/ESCRT-III proteins whose functional contributions are not yet clear.
Morita, Eiji, Sandrin, Virginie, McCullough, John, Katsuyama, Angela, Baci Hamilton, Ira, Sundquist, Wesley I.   +5 more
openaire   +2 more sources