A non-canonical ESCRT pathway, including histidine domain phosphotyrosine phosphatase (HD-PTP), is used for down-regulation of virally ubiquitinated MHC class I. [PDF]
, 2015 The Kaposi's sarcoma-associated herpes virus (KSHV) K3 viral gene product effectively down-regulates cell surface MHC class I. K3 is an E3 ubiquitin ligase that promotes Lys(63)-linked polyubiquitination of MHC class I, providing the signal for clathrin ...
Boname, Jessica M +7 more
core +2 more sources
Actin assembly ruptures the nuclear envelope by prying the lamina away from nuclear pores and nuclear membranes in starfish oocytes. [PDF]
, 2020 The nucleus of oocytes (germinal vesicle) is unusually large and its nuclear envelope (NE) is densely packed with nuclear pore complexes (NPCs) stockpiled for embryonic development. We showed that breakdown of this specialized NE is mediated by an Arp2/3-
Avilov, I. +6 more
core +1 more source
Cutaneous Melanoma Drives Metabolic Changes in the Aged Bone Marrow Immune Microenvironment
Aging and Cancer, EarlyView.Melanoma, the deadliest form of skin cancer, increasingly affects older adults. Our study reveals that melanoma induces changes in iron and lipid levels in the bone marrow, impacting immune cell populations and increasing susceptibility to ferroptosis.
Alexis E. Carey +12 more
wiley +1 more source
ESCRT Requirements for Murine Leukemia Virus Release
Viruses, 2016 The Murine Leukemia Virus (MLV) is a gammaretrovirus that hijack host components of the endosomal sorting complex required for transport (ESCRT) for budding.
Christina Bartusch, Reinhild Prange
doaj +1 more source
Syntaxin 16 is a master recruitment factor for cytokinesis [PDF]
, 2013 Recently it was shown that both recycling endosome and endosomal sorting complex required for transport (ESCRT) components are required for cytokinesis, in which they are believed to act in a sequential manner to bring about secondary ingression and ...
Alexandra Kaupisch +48 more
core +2 more sources
Structure of the AAA protein Msp1 reveals mechanism of mislocalized membrane protein extraction. [PDF]
, 2020 The AAA protein Msp1 extracts mislocalized tail-anchored membrane proteins and targets them for degradation, thus maintaining proper cell organization.
Myasnikov, Alexander +3 more
core +3 more sources
Isolation Defines Identity: Functional Consequences of Extracellular Vesicle Purification Strategies
Advanced Healthcare Materials, EarlyView.Four extracellular vesicle purification strategies are compared using ovarian‐cancer ascites and ES‐2 cell supernatants. A novel workflow links purification to function by combining particle‐normalized proteomics with matched cell‐free and cell‐based assays.
Christian Preußer +10 more
wiley +1 more source
SAXS Ensemble Refinement of ESCRT-III CHMP3 Conformational Transitions [PDF]
Structure, 2011 We developed and implemented an ensemble-refinement method to study dynamic biomolecular assemblies with intrinsically disordered segments. Data from small angle X-ray scattering (SAXS) experiments and from coarse-grained molecular simulations were combined by using a maximum-entropy approach.
B. Rozycki, Y. C. Kim, and G. Hummer
openaire +4 more sources
Structural and Functional Characterization of EXPO‐Derived Extracellular Vesicles in Plants
Advanced Science, EarlyView.In this study, 3D electron tomography (ET), cryo‐ET, and immunogold transmission electron microscopy (TEM) are employed to characterize plant extracellular vesicles (EVs) under physiological conditions. EVs are classified into three distinct categories according to their size, content, and molecular‐marker profiles. Furthermore, Exo70E2‐positive medium
Jiayang Gao +12 more
wiley +1 more source
Structural basis for activation, assembly and membrane binding of ESCRT-III Snf7 filaments
eLife, 2015 The endosomal sorting complexes required for transport (ESCRTs) constitute hetero-oligomeric machines that catalyze multiple topologically similar membrane-remodeling processes.
Shaogeng Tang +7 more
doaj +1 more source