Results 61 to 70 of about 84,275 (305)

Evaluation of plasmodesmata permeability by FRAP.

, 2022
(A,B) Representative images of fluorescence recovery after photobleaching (FRAP) of BY-2 cells expressing green fluorescent protein (GFP) with cycloheximide. (A) Fluorescence recovery of filament, which only bleached a part.
Kazunori Shimizu (149471), Ryo Tabata (730591), Masahiro Kikkawa (12418659), Hiroyuki Honda (4713), Daisuke Kurihara (3975116), Ken-ichi Kurotani (12418656), Michitaka Notaguchi (8652267), Yaichi Kawakatsu (827407)   +7 more
core   +1 more source

Endoplasmic Reticulum Geometry Dictates Neuronal Bursting via Calcium Store Refill Rates and Exposes Selective Neuronal Vulnerability

Advanced Science, EarlyView.
The ER's continuous tubular network is maintained by ER‐shaping proteins whose mutation or dysregulation contributes to neurodegenerative diseases. Here, we show that ER morphology sets the speed of Ca2+ store replenishment between firing events. Disrupting ER continuity slows intra‐ER Ca2+ redistribution from extracellular refill (SOCE) sites, driving
Valentina Davi, Pierre Parutto, Yuyi Zhang, Tasuku Konno, Cecile Crapart, Raquel Pereira, John P. Franklin, Mosab Ali Awadelkareem, Daniel C Maddison, Michael J. Devine, Edgar R. Gomes, Joseph Chambers, Elena Koslover, Edward Avezov   +13 more
wiley   +1 more source

Quantification of the Antioxidant Activity of Plant Extracts: Analysis of Sensitivity and Hierarchization Based on the Method Used

Antioxidants, 2020
Plants have a large number of bioactive compounds with high antioxidant activity. Studies for the determination of the antioxidant activity of different plant species could contribute to revealing the value of these species as a source of new antioxidant
Natividad Chaves, Antonio Santiago, Juan Carlos Alías   +2 more
doaj   +1 more source

Summary of FRAP experiment analysis.

, 2013
Summary of FRAP experiment analysis.
Laurent Guillon (477979), Stephan Altenburger (477980), Peter L. Graumann (109027), Isabelle J. Schalk (477981)   +3 more
core   +1 more source

Biolipid Film‐Fused Electrochemiluminescence for Multipurpose In Situ Bioassays

Advanced Science, EarlyView.
An ECL‐emissive, membrane‐interactive scaffold was fabricated, and facilely fused with natural and non‐native phospholipids into multifactorial mimicries of cytomembranes and vesicles for in vitro representative membrane‐process probing. Such a biointerface‐based, state‐sensitive ECL paradigm not only pinpointed proximal phenomena, including channeling
Jialiang Chen, Bin Li, Yingying Wang, Fulin Zhu, Wenxuan Yang, Xuanyu Fu, Tiantian Man, Qiuyue Wu, Kewei Ren, Shengyuan Deng   +9 more
wiley   +1 more source

Distributed Actin Turnover in the Lamellipodium and FRAP Kinetics [PDF]

Biophysical Journal, 2013
Studies of actin dynamics at the leading edge of motile cells with single-molecule speckle (SiMS) microscopy have shown a broad distribution of EGFP-actin speckle lifetimes and indicated actin polymerization and depolymerization over an extended region.
Smith, Matthew B., Kiuchi, Tai, Watanabe, Naoki, Vavylonis, Dimitrios   +3 more
openaire   +2 more sources

FRAP fluorescence vs. time.

, 2017
Representative FRAP curve showing the recovery of fluorescence with time after the photobleach, which ended at t = 0. Red points/line are the experimental data, and the blue points/line are the best fit to the data using Eq (2).
Karin Scarpinato (3710359), Josiah Low (3710356), Tracey Pu (3710353), Daniel Nelli (3710344), Justin Sigley (3710347), Martin Guthold (270306), Keith Bonin (227243), John Jarzen (3710350)   +7 more
core   +1 more source

Fine‐Tuned Regulation of mRNA Translation and Transport by STAU2 Condensate Facilitates Neuronal Development and Plasticity

Advanced Science, EarlyView.
STAU2 undergoes phase separation to form dynamic condensates that package target mRNAs and deliver them to the distal ends of growing neuronal dendrites. STAU2 condensates stabilize embedded mRNAs and repress their translation. Synaptic activity bidirectionally remodels STAU2 condensates, coordinating local translation of STAU2‐associated mRNAs ...
Shijing Huang, Yuanyuan Chu, Ruiqian Zhao, Siyu Li, Yu Feng, Hongdan Zheng, Yunli Xie, Tong Wang, Wenyu Wen   +8 more
wiley   +1 more source

ahansenlab/FRAP-drift-correction-GUI: First release of FRAP-drift-correction-GUI

<p>This is the first release of the FRAP drift correction GUI used in the paper "An autoinhibitory switch of the LSD1 disordered region controls enhancer silencing".</p ...
DomenicN
core   +1 more source

Oligopeptides/DNA Coacervate Droplets as Macromolecular Delivery Microcarriers

Advanced Science, EarlyView.
Oligopeptide–DNA coacervates formed via liquid–liquid phase separation serve as programmable carriers for macromolecular cargos, entering cells through lipid raft–associated pathways and subsequently undergoing enzyme triggered intracellular disassembly driven by DNA degradation processes, thereby enabling efficient, controlled, and spatially defined ...
Linyi Zhang, Chong Wang, Mengqi Han, Yi Yang, Teng Ma, Luoran Shang   +5 more
wiley   +1 more source