T-cell responses targeting HIV Nef uniquely correlate with infected cell frequencies after long-term antiretroviral therapy. [PDF]
PLoS Pathogens, 2017 HIV-specific CD8+ T-cell responses limit viral replication in untreated infection. After the initiation of antiretroviral therapy (ART), these responses decay and the infected cell population that remains is commonly considered to be invisible to T-cells.
Allison S Thomas +18 more
doaj +1 more source
Biosynthesis of rumbrins and inspiration for discovery of HIV inhibitors
Acta Pharmaceutica Sinica B, 2022 Investigation on how nature produces natural compounds with chemical and biological diversity at the genetic level offers inspiration for the discovery of new natural products and even their biological targets.
Beifen Zhong +7 more
doaj +1 more source
Expression and biochemical characterization of human immunodeficiency virus type 1 nef gene product [PDF]
Journal of Virology, 1990 nef genes from human immunodeficiency virus type 1 isolates BH10 and LAV1 (lymphadenopathy-associated virus type 1) were expressed in Escherichia coli under the deo operon promoter. The two proteins found in the soluble compartment of the bacterial lysate were purified by ion-exchange column chromatography to apparent homogeneity.
J, Kaminchik +9 more
openaire +2 more sources
Expression, purification and biochemical characterisation of the human immunodificiency virus 1 nef gene product [PDF]
European Journal of Biochemistry, 1992 The human immunodeficiency virus 1 (HIV‐1) nef gene encoded by the HIV‐1 isolate lymphadenopathy‐associated virus type 1 was expressed in Escherichia coli under the control of the tac promoter. The protein is found mainly in the soluble part of the bacterial lysate; a simple twocolumn purification scheme has been developed allowing isolation of the ...
Wolber, V. +6 more
openaire +4 more sources
Evidence of differential HLA class I-mediated viral evolution in functional and accessory/regulatory genes of HIV-1. [PDF]
PLoS Pathogens, 2007 Despite the formidable mutational capacity and sequence diversity of HIV-1, evidence suggests that viral evolution in response to specific selective pressures follows generally predictable mutational pathways.
Zabrina L Brumme +16 more
doaj +1 more source
Signature molecules expressed differentially in a liver disease stage-specific manner by HIV-1 and HCV co-infection. [PDF]
PLoS ONE, 2018 To elucidate HIV-1 co-infection-induced acceleration of HCV liver disease and identify stage-specific molecular signatures, we applied a new high-resolution molecular screen, the Affymetrix GeneChip Human Transcriptome Array (HTA2.0), to HCV-mono- and ...
Amanda Whitmill +7 more
doaj +1 more source
Construction and characterization of highly infectious full-length molecular clones of a HIV-1 CRF07_BC isolate from Xinjiang, China. [PDF]
PLoS ONE, 2013 Among the various subtypes of the M group of human immunodeficiency virus type 1 (HIV-1), clade CRF07_BC is the most prevalent in China. To date, no strong replicable CRF07_BC infectious clone has been constructed.
Zheng Wang +7 more
doaj +1 more source
A recombinant herpesviral vector containing a near-full-length SIVmac239 genome produces SIV particles and elicits immune responses to all nine SIV gene products. [PDF]
PLoS Pathogens, 2018 The properties of the human immunodeficiency virus (HIV) pose serious difficulties for the development of an effective prophylactic vaccine. Here we describe the construction and characterization of recombinant (r), replication-competent forms of rhesus ...
Young C Shin +8 more
doaj +1 more source
JCI Insight, 2021 Antiretroviral therapies (ARTs) abrogate HIV replication; however, infection persists as long-lived reservoirs of infected cells with integrated proviruses, which reseed replication if ART is interrupted.
Eva M. Stevenson +23 more
doaj +1 more source
Genetic characterization of human immunodeficiency virus type 1 nef gene products translated in vitro and expressed in mammalian cells [PDF]
Journal of Virology, 1991 Expression of the human immunodeficiency virus type 1 nef gene was studied by in vitro transcription-translation and by transfection into monkey COS cells. Two Nef-related peptides, of 27 and 25 kDa, were identified by immunoprecipitation with anti-Nef antibodies.
J, Kaminchik +5 more
openaire +2 more sources