Genotyping-by-sequencing: comparison to SNP chips and whole genome sequencing
, 2015 Outline: Animal Productivity Group; Animal Genomics Team; Genomics Tool Kit; From tissue sample to breeding values; Sheep genome re-sequencing; High Density (HD) SNP Chip development; Genotyping-by-sequencing; GBS-Bioinformatics pipeline; Comparison ...
Rudiger Brauning (47247) +4 more
core +1 more source
SNP types identified in this study through genotyping-by-sequencing.
, 2018 SNP types identified in this study through genotyping-by-sequencing.
Todd C. Mockler (110557) +8 more
core +1 more source
Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy
Molecular Oncology, EarlyView.We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley +1 more source
Genotyping-by-sequencing (GBS): a novel, efficient and cost-effective genotyping method for cattle using next-generation sequencing. [PDF]
PLoS ONE, 2013 High-throughput genotyping methods have increased the analytical power to study complex traits but high cost has remained a barrier for large scale use in animal improvement. We have adapted genotyping-by-sequencing (GBS) used in plants for genotyping 47
Marcos De Donato +4 more
doaj +1 more source
Concordance between genotyping-by-sequencing and KASP genotyping calls.
, 2013 Concordance between genotyping-by-sequencing and KASP genotyping calls.
Herman J. van Eck (411319) +5 more
core +1 more source
Spatial heterogeneity of hotspot mutations and clonal relatedness in ovarian endometrioid carcinoma
Molecular Oncology, EarlyView.Spatially resolved analysis revealed heterogeneous distributions of selected cancer‐associated mutations within ovarian endometrioid carcinomas. Shared mutations between paired endometrial and ovarian tumors supported clonal relatedness. These findings highlight the value of sampling multiple tumor regions when interpreting molecular profiles ...
Takahito Ashihara +10 more
wiley +1 more source
Molecular Oncology, EarlyView.Enterotoxigenic Bacteroides fragilis is a gut bacterium linked to bowel cancer. We propose that its toxin acts along two routes: at the cell surface, cutting the adhesion protein E‐cadherin and triggering inflammation; and inside the cell, delivered by bacterial vesicles, switching on NOD1 and cancer stem cell programs.
Kangchan Choi +2 more
wiley +1 more source
Genome-wide association studies of seven agronomic traits under two sowing conditions in bread wheat
BMC Plant Biology, 2019 Background Wheat is a cool seasoned crop requiring low temperature during grain filling duration and therefore increased temperature causes significant yield reduction.
Muhammad Jamil +8 more
doaj +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Number and chromosome location of SNPs discovered by genotyping-by-sequencing of 178 rye accessions.
, 2019 Number and chromosome location of SNPs discovered by genotyping-by-sequencing of 178 rye accessions.
Jagdeep Singh Sidhu (6523880) +7 more
core +1 more source