Results 91 to 100 of about 56,079,681 (305)
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Genotyping-by-Sequencing application in Chinook Salmon
, 2016 Genomics and DNA sequencing technologies are rapidly advancing to the point where they can be cost effectively applied within aquaculture breeding programmes.
Hayley Baird (8845760) +5 more
core
Genetic diversity analysis of lettuce using genotyping-by-sequencing
, 2021 Genome-wide SNP discovery and population structure analysis in lettuce (Lactuca Sativa) were conducted using genotyping by ...
Beiquan Mou USDA (17856947) +2 more
core
Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes
FEBS Open Bio, EarlyView.Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard +12 more
wiley +1 more source
Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region
FEBS Open Bio, EarlyView.Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson +2 more
wiley +1 more source
Evolution‐guided yeast complementation reveals functional differences in human PSPH variants
FEBS Open Bio, EarlyView.Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez +6 more
wiley +1 more source
Reproducibility of Genotyping-by-Sequencing
, 2015 Genotyping-by-Sequencing is a high-throughput genotyping platform that promises to tap into complex genomes, especially genomes of obligate outcrossing species.
Marty Faville (5072459) +2 more
core
Quantitative Genetic Mapping and Genome Assembly in the Lesser Wax Moth Achroia grisella
G3: Genes, Genomes, Genetics, 2019 Specific characteristics of the male Achroia grisella acoustic mating signal determine a male’s attractiveness toward females. These features are genetically variable in populations, and mapping experiments have been used to identify loci contributing to
Boryana S. Koseva +7 more
doaj +1 more source
FEBS Open Bio, EarlyView.The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source
, 2017 Next-generation sequencing is becoming increasingly accessible to researchers asking biosystematic questions, but current best practice in both choosing a specific approach and effectively analysing the resulting data set is still being explored.
Siegfried L. Krauss (296804) +7 more
core +1 more source