Results 81 to 90 of about 58,027,105 (302)
, 2016 Genotyping-by-sequencing: Emerging alternative technology to array based genotyping for GBLUP & GWAS. Two “categories” - oligo directed re-sequencing of specific regions -targeted; reduced representational re-sequencing- restriction enzyme digests ...
Ken Dodds (3501542) +3 more
core
Spatial heterogeneity of hotspot mutations and clonal relatedness in ovarian endometrioid carcinoma
Molecular Oncology, EarlyView.Spatially resolved analysis revealed heterogeneous distributions of selected cancer‐associated mutations within ovarian endometrioid carcinomas. Shared mutations between paired endometrial and ovarian tumors supported clonal relatedness. These findings highlight the value of sampling multiple tumor regions when interpreting molecular profiles ...
Takahito Ashihara +10 more
wiley +1 more source
Frontiers in Plant Science, 2018 Sweetpotato (Ipomoea batatas) plays a critical role in food security and is the most important root crop worldwide following potatoes and cassava. In the United States (US), it is valued at over $700 million USD.
Phillip A. Wadl +6 more
doaj +1 more source
BMC Plant Biology, 2020 Background Continuous storage root formation and bulking (CSRFAB) in sweetpotato is an important trait from agronomic and biological perspectives. Information about the molecular mechanisms underlying CSRFAB traits is lacking.
Astère Bararyenya +14 more
doaj +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Genotyping-by-Sequencing: efficiency and reproducibility checks
, 2015 Genotyping-by-Sequencing (GBS) has the potential to be a cost effective, reproducible and high-throughput SNP genotyping method. We have been investigating GBS in a number of livestock species, with an emphasis on Sheep.
Rudiger Brauning (47247) +4 more
core
CTAB DNA Extraction and Genotyping-by-Sequencing to Map Meiotic Crossovers in Plants
, 2022 Reciprocal DNA crossovers between chromosomes form new allelic combinations and contribute to the formation of novel genetic diversity. Crossovers are formed during meiosis of germ cells and these recombination events have influenced plant genome ...
Lambing, C. +4 more
core +1 more source
Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes
FEBS Open Bio, EarlyView.Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard +12 more
wiley +1 more source
Genotyping-by-Sequencing - Development in livestock
, 2015 Recent advances in next generation sequencing technology have increased the output/cost to a level that where genotyping-by-sequencing (GBS) in livestock and plant crops as well as aquaculture species can be considered. GBS has the potential to be a cost
Rudiger Brauning (47247) +4 more
core