Results 51 to 60 of about 6,118 (218)

Mass Spectrometry Strategies for O-Glycoproteomics

Cells
Glycoproteomics has accelerated in recent decades owing to numerous innovations in the analytical workflow. In particular, new mass spectrometry strategies have contributed to inroads in O-glycoproteomics, a field that lags behind N-glycoproteomics due ...
Amanda Helms, Jennifer S. Brodbelt
doaj   +1 more source

Noncanonical Amino Acids in the Interrogation of Cellular Protein Synthesis [PDF]

, 2011
Proteins in living cells can be made receptive to bioorthogonal chemistries through metabolic labeling with appropriately designed noncanonical amino acids (ncAAs).
Ngo, John T., Tirrell, David A.
core   +2 more sources

A comprehensive N-glycoproteome atlas reveals tissue-specific glycan remodeling but non-random structural microheterogeneities

Nature Communications
The mouse is a key model in biomedical research, yet its tissue-specific glycoproteome remains incompletely characterized due to glycan complexity and microheterogeneity.
Yongqi Wu, Muyao Yang, Yongchao Xu, Li Jia, Jun Li, Xiaohan Wang, Jiayu Zhao, Yinli Cai, Yiwen Zhang, Shisheng Sun   +9 more
doaj   +1 more source

Studying Macromolecular Composition in Cell–Cell Interfaces Using 3D Membrane Reconstitution Systems

Advanced Science, EarlyView.
A comprehensive understanding of the interactions between proteins, lipids and glycocalyx components at the immune synapse is still lacking. Here, an artificial and a semi‐artificial model contact system were established to reconstitute the cell‐cell contact in 3D. The model systems enable the examination of macromolecule enrichment or depletion at the
Franziska Ragaller, Amelie Maribel Schneider, Ellen Sjule, Renhua Sun, Xiao Han, Luca Andronico, Edward Jenkins, Michael Dustin, Adnane Achour, Erdinc Sezgin   +9 more
wiley   +1 more source

Differentiation of mucinous from non-mucinous pancreatic cyst fluid using dual-stained, 1 dimensional polyacrylamide gel electrophoresis [PDF]

, 2014
BACKGROUND: Pancreatic cysts are being increasingly identified in patients. Mucinous cysts have malignant potential whereas non-mucinous cysts do not.
John C Deutsch, John M Streitz, Joseph L Deutsch, Kirk P Bernadino, Michael T Madden, Wilmar Salo   +5 more
core   +1 more source

A Linkage-specific Sialic Acid Labeling Strategy Reveals Different Site-specific Glycosylation Patterns in SARS-CoV-2 Spike Protein Produced in CHO and HEK Cell Substrates

Frontiers in Chemistry, 2021
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus utilizes the extensively glycosylated spike (S) protein protruding from the viral envelope to bind to angiotensin-converting enzyme-related carboxypeptidase (ACE2) as its primary ...
Qiong Wang, Yan Wang, Shuang Yang, Shuang Yang, Changyi Lin, Lateef Aliyu, Yiqun Chen, Lisa Parsons, Yuan Tian, Hongpeng Jia, Andrew Pekosz, Michael J. Betenbaugh, John F. Cipollo   +12 more
doaj   +1 more source

Sustainable Phosphorylated Cellulose Nanocrystals: A Dual‐Affinity Platform for High‐Efficiency Enrichment of Intact Glycopeptides and Phosphopeptides

Advanced Science, EarlyView.
AAA ABSTRACT Protein glycosylation and phosphorylation are critical post‐translational modifications (PTMs) governing nearly all cellular functions, yet their analysis remains challenging due to reliance on costly and unsustainable enrichment materials.
Jiaying Li, Wuming Fan, Xuyang Yue, Dongdong Wang, Xiangyu Tang, Zhuo Zhang, Yonggui Wang, Yanjun Xie, Mingliang Ye, Hongqiang Qin   +9 more
wiley   +1 more source

Precision N-Glycoproteomic Profiling of Murine Peritoneal Macrophages After Different Stimulations

Frontiers in Immunology, 2021
Macrophages are important immune cells that participate in both innate and adaptive immune responses, such as phagocytosis, recognition of molecular patterns, and activation of the immune response.
Lujie Yang, Tianqi Gong, Huali Shen, Jiangnan Pei, Lei Zhang, Quanqing Zhang, Yuanyu Huang, Zuojian Hu, Ziyue Pan, Pengyuan Yang, Ling Lin, Hongxiu Yu   +11 more
doaj   +1 more source

Mimic, target, and adapt: Cell membrane‐coated nanoplatforms for precision and immune‐guided cancer treatment

BMEMat, EarlyView.
This Perspective explores the emerging landscape of cell membrane‐coated nanoparticles (CM‐NPs) as intelligent, immune‐compatible platforms for cancer therapy. Highlighting design strategies, translational challenges, and competitive positioning, it outlines how integrating biomimetic targeting with advanced analytical and manufacturing tools could ...
A. K. M. M. Alam, Xiaojun Cai, Malik Ihsan Ullah Khan, Robert Musiol, Quazi T. H. Shubhra   +4 more
wiley   +1 more source

Comparative glycoproteomics: approaches and applications [PDF]

Briefings in Functional Genomics and Proteomics, 2008
Glycosylation plays fundamental roles in controlling various biological processes. Therefore, glycosylation analysis has become an important target for proteomic research and has great potential for clinical applications. With the continuous development and refinement of glycoprotein isolation methods, increasing attention has been directed to the ...
Xin, Wei, Lingjun, Li
openaire   +2 more sources