Results 51 to 60 of about 5,264,018 (278)

The Neuron Navigators: Structure, function, and evolutionary history

Frontiers in Molecular Neuroscience, 2023
Neuron navigators (Navigators) are cytoskeletal-associated proteins important for neuron migration, neurite growth, and axon guidance, but they also function more widely in other tissues.
Regina M. Powers, Regina M. Powers, Robert F. Hevner, Robert F. Hevner, Shelley Halpain, Shelley Halpain   +5 more
doaj   +1 more source

Spatial and single‐nuclei transcriptomics reveals idiosyncratic and generic patterns in papillary and anaplastic thyroid cancers

Molecular Oncology, EarlyView.
Matched spatial transcriptomics and single‐nuclei RNA‐seq were generated for anaplastic and BRAFV600E papillary thyroid cancers revealing generic and tumor‐specific states occurring in cancer cells and in the tumor microenvironment. In this context, cancer dedifferentiation mirrored organoid maturation through ordered thyroid marker gain/loss ...
Adrien Tourneur, Joel Rodrigues Vitória, Manuel Saiselet, Ligia Craciun, Denis Larsimont, Anne Lefort, Frédérick Libert, Carine Maenhaut, Sabine Costagliola, Maxime Tarabichi, Mirian Romitti, Vincent Detours   +11 more
wiley   +1 more source

Much More Than a Scaffold: Cytoskeletal Proteins in Neurological Disorders

Cells, 2020
Recent observations related to the structure of the cytoskeleton in neurons and novel cytoskeletal abnormalities involved in the pathophysiology of some neurological diseases are changing our view on the function of the cytoskeletal proteins in the ...
Diana C. Muñoz-Lasso, Carlos Romá-Mateo, Federico V. Pallardó, Pilar Gonzalez-Cabo   +3 more
doaj   +1 more source

Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3A

eLife, 2021
Variants in the high confident autism spectrum disorder (ASD) gene ANK2 target both ubiquitously expressed 220 kDa ankyrin-B and neurospecific 440 kDa ankyrin-B (AnkB440) isoforms.
Blake A Creighton, Simone Afriyie, Deepa Ajit, Cristine R Casingal, Kayleigh M Voos, Joan Reger, April M Burch, Eric Dyne, Julia Bay, Jeffrey K Huang, ES Anton, Meng-Meng Fu, Damaris N Lorenzo   +12 more
doaj   +1 more source

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source

Revisiting Netrin-1: One Who Guides (Axons)

Frontiers in Cellular Neuroscience, 2018
Proper patterning of the nervous system requires that developing axons find appropriate postsynaptic partners; this entails microns to meters of extension through an extracellular milieu exhibiting a wide range of mechanical and chemical properties. Thus,
Nicholas P. Boyer, Stephanie L. Gupton, Stephanie L. Gupton   +2 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Nystatin Regulates Axonal Extension and Regeneration by Modifying the Levels of Nitric Oxide

Frontiers in Molecular Neuroscience, 2020
Nystatin is a pharmacological agent commonly used for the treatment of oral, mucosal and cutaneous fungal infections. Nystatin has also been extensively applied to study the cellular function of cholesterol-enriched structures because of its ability to ...
Cristina Roselló-Busquets, Cristina Roselló-Busquets, Marc Hernaiz-Llorens, Marc Hernaiz-Llorens, Eduardo Soriano, Eduardo Soriano, Eduardo Soriano, Ramon Martínez-Mármol   +7 more
doaj   +1 more source

A new view of axon growth and guidance grounded in the stochastic dynamics of actin networks

Open Biology, 2023
The mechanism of axon growth and guidance is a core, unsolved problem in neuroscience and cell biology. For nearly three decades, our view of this process has largely been based on deterministic models of motility derived from studies of neurons cultured
Rameen Forghani, Aravind Chandrasekaran, Garegin Papoian, Edward Giniger   +3 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source